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        Ethylene Production and Expression of Two Ethylene Biosynthetic Genes in Senescing Flowers of Hosta ventricosa

        Zhu, Xiaoxian,Hu, Haitao,Guo, Weidong,Chen, Jianhua,Wang, Changchun,Yang, Ling Korean Society of Horticultural Science 2014 원예과학기술지 Vol.32 No.2

        Senescence of Hosta ventricosa flowers was firstly characterized as ethylene-sensitive since the deterioration of the tepal was accompanied by increased endogenous ethylene biosynthesis. The full-length cDNAs and DNAs of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) and ACC oxidase (ACO) involved in ethylene biosynthesis were cloned from H. ventricosa flowers. The HvACS ORF with 1347 bp and two introns, encoded a polypeptide of 448 amino acids showing 79% homology with that in Musa acuminata. The HvACO ORF contained 957 bp and three introns, encoding a 318-residue polypeptide showing 83% homology with that in Narcissus tazetta. The timing of the induction of HvACS expression was in correspond to the timing of the increase in ethylene production, and that the up-regulation of HvACO transcript was closely correlated with an elevated ethylene production, but underwent a down-regulation in wounded leaves with elevated ethylene emission. The results, together with expression analysis in vegetative tissues, suggested that both HvACS and HvACO were specifically regulated by flower senescence.

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        Ethylene Production and Expression of Two Ethylene Biosynthetic Genes in Senescing Flowers of Hosta ventricosa

        Xiaoxian Zhu,Haitao Hu,Weidong Guo,Jianhua Chen,Changchun Wang,Ling Yang 한국원예학회 2014 원예과학기술지 Vol.32 No.2

        Senescence of Hosta ventricosa flowers was firstly characterized as ethylene-sensitive since the deterioration of the tepal was accompanied by increased endogenous ethylene biosynthesis. The full-length cDNAs and DNAs of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) and ACC oxidase (ACO) involved in ethylene biosynthesis were cloned from H. ventricosa flowers. The HvACS ORF with 1347 bp and two introns, encoded a polypeptide of 448 amino acids showing 79% homology with that in Musa acuminata. The HvACO ORF contained 957 bp and three introns, encoding a 318-residue polypeptide showing 83% homology with that in Narcissus tazetta. The timing of the induction of HvACS expression was in correspond to the timing of the increase in ethylene production, and that the up-regulation of HvACO transcript was closely correlated with an elevated ethylene production, but underwent a down-regulation in wounded leaves with elevated ethylene emission. The results, together with expression analysis in vegetative tissues, suggested that both HvACS and HvACO were specifically regulated by flower senescence.

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        Preparation and evaluation of porous H1.6Mn1.6O4@chitosan pellet for Li+ extraction

        Xiaoxian Zhang,Yue Niu,Feng Xue,Jianhong Gao,Xiaolei Zhu,Shengui Ju 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.10

        Spinel-structured lithium manganese oxide is regarded as one of the most promising materials that can recover Li+ from brine and seawater. Herein, a hierarchical porous and hydrophilic H1.6Mn1.6O4@chitosan pellet (HMO@CP) is proposed and its mechanical property is tailored through the glutaraldehyde-derived cross-linking. Different characterization techniques such as scanning electron microscopy (SEM), Brunner-Emmet-Teller (BET) measurement, Fourier transformation infrared spectrum (FTIR), and X-ray diffraction (XRD) meter were used to investigate the chemical and morphological properties of the HMO@CP. H1.6Mn1.6O4 powders were successfully encapsulated by chitosan, forming composite porous pellets. The equilibrium adsorption capacity of HMO@CP is 49.2mg·g1, which is similar to that of the pristine H1.6Mn1.6O4. Moreover, the adsorption behavior of HMO@CP well fits with the pseudosecond- order kinetic model, and the Langmuir model can be used to describe the adsorption isotherm of HMO@CP. Furthermore, the adsorption thermodynamic parameters such as H, G and S were calculated based on the obtained results. When the pellet is immersed into 0.05mol·L1 HCl solution after the Li+ adsorption process, the desorption equilibrium can be reached within 60 min, with a manganese dissolution loss of 2.48%. The Li+ adsorption capacity of HMO@CP remains at 41.92mg·g1 after five adsorption-desorption cycles, confirming the effective regeneration property of the HMO@CP. In addition, the as-prepared HMO@CP shows excellent selectivity for Li+ among Na+, K+, Mg2+, and Ca2+ ions in the simulated solution.

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