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Selective Adsorption and Reusability for Pb<SUP>2+</SUP> of Chitosan-based Microporous Polymer
Jie Yu,Jidong Zheng,Quanfang Lu,Xing Wang,Xiaomin Zhang,Qizhao Wang,Wu Yang 한국고분자학회 2017 폴리머 Vol.41 No.3
A green and simple glow-discharge electrolysis plasma (GDEP) technique was used to synthesize the chitosan/attapulgite/poly(acrylic acid-co-2-acrylamido-2-methyl-1-propanesulfonic acid) (CS/ATP/P(AA-co-AMPS)) microporous polymer. The results showed that the optimum pH for the adsorption of Pb<SUP>2+</SUP>, Cd<SUP>2+</SUP>, Co<SUP>2+</SUP>, and Cu<SUP>2+</SUP> is 4.8, and time of adsorption equilibrium is 60 min. Adsorption kinetics fits well to the pseudo-second-order model. The maximum adsorption capacities for Pb<SUP>2+</SUP>, Cd<SUP>2+</SUP>, Cu<SUP>2+</SUP> and Co<SUP>2+</SUP> are 500.0, 301.7, 180.0 and 151.7 mg g<SUP>-1</SUP>, respectively. The microporous polymer has higher adsorption selectivity toward Pb<SUP>2+</SUP> with the coexistence of Cd<SUP>2+</SUP>, Co<SUP>2+</SUP>, and Cu<SUP>2+</SUP>. The CS/ATP/P(AAco-AMPS) possesses promising regeneration and reusability using 2.0 g L–1 ethylenediamine tetraacetic acid tetrasodium salt (EDTA-4Na) solution as eluent. CS/ATP/P(AA-co-AMPS) can be used as a very promising absorbent for the separation purification and selective recovery of Pb<SUP>2+</SUP> in aqueous solution containing Cd<SUP>2+</SUP>, Co<SUP>2+</SUP>, and Cu<SUP>2+</SUP> ions.
Jingjing Li,Shuang Wang,Bo Wang,Hao Wei,Xin Liu,Jun Hao,Yanping Duan,Jinlian Hua,Xiaomin Zheng,Xiuliang Feng,Xingrong Yan 한국통합생물학회 2018 Animal cells and systems Vol.22 No.4
Global human health has been compromised by high-fat diets. This study aimed to investigate the relationship between a high-fat diet and parthenogenetic embryo quality. Mice fed a high-fat or a normal diet was used as treated or control groups, respectively. Estradiol (E2), total cholesterol (TC) and total triglyceride (TG) were detected by Enzyme-Linked ImmunoSorbent Assay (ELISA). Cumulus-oocyte complexes (COCs) were collected from the mice in the treated and control groups. The ultrastructure of COCs, the expression level of genes involved in mitochondrial and nuclear functions in cumulus cells and oocytes quality were evaluated with transmission electron microscopy, real-time quantitative polymerase chain reaction (RT-PCR) and artificial parthenogenesis, respectively. The results showed that the efficiency of parthenogenetic embryonic development in vitro was significantly higher in the treated group than in the control group (p < .05). The expression level of genes involved in mitochondrial function was lower in cumulus cells from the treated group than that from the control group (p < .05). The estradiol and cholesterol level in the serum and the expression level of P450 arom were higher in the treated group than the control group (p < .05). The reactive oxygen species (ROS) level was higher in culumus cells from the treated group than the control group, while the mitochondrial membrane potential was lower in cumulus cells from the treated group (p < .05). Accumulation of lipid droplets was only in cumulus but in oocyte, the results demonstrated that mitochondrial functions were impaired by a high-fat diet, but parthenogenetic embryonic development in vitro was improved, in controllable range of damage for the body.
Xiaojiao Sima,Bo Jiang,Jia Fang,Yongqin He,Zhongxiang Fang,Saravana Kumar KM,Wei Ren,Lingling Qiu,Xiaomin Chen,Bingsong Zheng 한국식물생명공학회 2015 Plant biotechnology reports Vol.9 No.3
MicroRNAs (miRNAs) play a vital role in plant development and growth through negative regulation of post-transcriptional gene expression. Carya cathayensis (hickory) is an important species for dried nuts and oil in China, with high nutritional and economic value. The graft technique is an important strategy for hickory cultivation. To understand the role of miRNAs involved in the hickory graft process, we constructed three small ribonucleic acid (RNA) libraries from hickory rootstock (2 years old) and scion (1 year old) at 0, 7, and 14 days post-graft. Sequence analysis of the three libraries identified 21 conserved miRNAs belonging to 13 families, and 10 novel and 8 potentially novel miRNAs belonging to 15 families. Among these miRNAs, 12 miRNAs were differentially expressed during the graft process in hickory and twothirds were downregulated. Quantitative real-time polymerase chain reaction validated that 14 miRNAs and their expression trends were similar to the results obtained by Solexa sequencing. Further, a total of 89 target genes for conserved and 26 target genes for novel miRNAs were predicted. This study will help in understanding the roles and regulatory modes of miRNAs involvement in the hickory graft process.