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        Variation in limonin and nomilin content in citrus fruits of eight varieties determined by modified HPLC

        Shengjia Huang,Xinya Liu,Bo Xiong,Xia Qiu,Guochao Sun,Xiaojia Wang,Xu Zhang,Zhixiang Dong,Zhihui Wang 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.3

        The nomilin and limonin content in citrus fruitsof different varieties was determined at fruit growth andmaturation stages by HPLC. The results showed that thetwo limonoids can be separated, identified, and quantifiedin citrus fruits within 10 min by the developed method. Themethod exhibited good precision, repeatability, stability,and recovery rate. The content of limonin and nomilin inmost citrus fruits presented an increasing trend initially,and then decreased during fruit growth and maturation; apeak was observed at the young fruit or fruit expansionstage. The dropped fruits also contained some amount oflimonoids, suggesting their industrial application. Thevariation and cluster analyses results revealed that theorange varieties contained the highest amount of limonoidsat the mature stage. The results of this study will enablebetter use of citrus limonoids.

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        Development of seven SNP molecular markers based on the key genes related to red colour of fruit skin in Japanese apricot (Prunus mume)

        Ni Xiaojia,Iqbal Shahid,Xue Song,Ni Zhaojun,Huang Yinghong,Gao Zhihong 한국원예학회 2023 Horticulture, Environment, and Biotechnology Vol.64 No.6

        Fruit color is an important factor that aff ects its quality. The red-skinned cultivars of Japanese apricot ( Prunus mume Sieb. et Zucc.) have high medicinal economic benefi ts and are mainly used for deep-processed products because of their eye-catching red pigmentation. In contrast, molecular markers related to the red color trait in Japanese apricot fruit haven’t been reported. This study identifi ed twenty-eight SNPs markers based on genome resequencing data. The average number of alleles per SNP marker site in red and green-skinned groups of Japanese apricot cultivars was 1.9643, with heterozygosity ranging from 0.02 to 0.75, and an average Shannon index of 0.517 and 0.4420, respectively, indicating high dispersion and diversity. The total germplasm was divided into two (K = 2) clusters, including 10 and 34. Most red-skinned Japanese apricot cultivars were classifi ed as cluster I, showing the signifi cant genetic diff erence between the two cultivar groups ( Fst = 0.54). According to PIC values, a total of seven SNPs markers showed high polymorphism ( PIC > 0.5), including PmSNP_1 ( 4-CL ), PmSNP_4 ( WD40 ), PmSNP_5 ( MYB29 ), PmSNP_18 ( UFGT6 ) and PmSNP_27 ( UFGT3 ) that were highly correlated with red color trait. Among them, PmUFGT3 exhibited the highest polymorphism, which is signifi cant for future research on developing and utilizing germplasm resources related to red fruit skin.

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        Modification of disused floral foam with polyethylene diamine for the removal of heavy metal ions

        Yilin Yu,Jindeng Huang,Yingmin Liao,Zhixiang Lin,Xiaojia Huang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.128 No.-

        Floral foam (FF) based on phenol formaldehyde resin has been used widely for many years, but it is reusedrarely and the discarded FF is harmful to environment. Therefore, recycling of disused FF is interestingand highly required. In current study, a new strategy for reutilization of disused FF was proposed. FFwhich had been treated with NaOH solution was dipped in polyethyleneimine (PEI) solution to anchoractive amino groups on the framework of FF, and then glutaraldehyde was employed to cross-link theanchored PEI to obtain a novel adsorbent based on disused FF (PEI@FF). Due to the abundant aminogroups, the prepared adsorbent displayed satisfactory chelating ability towards heavy metal ions. Under the optimal synthesis and adsorption conditions, the PEI@FF was applied to remove Cu(II), Cd(II) and Pb(II) in environmental waters, and the removal rates varied from 91.4% to 95.6%, which weremuch higher than that achieved on unmodified FF (35–43%). Additionally, the PEI@FF exhibited goodbatch-to-batch preparation repeatability and acceptable number of cyclic utilization. The satisfactoryresults well evidence the proposed method is practicable for reutilization of discarded FF, and the presentstudy also supplies a new adsorbent for the high efficient removal of heavy metal ions.

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