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Choe, Youngji,Yu, Ji‐,Yeon,Son, Young‐,Ok,Park, Seung‐,Moon,Kim, Jong‐,Ghee,Shi, Xianglin,Lee, Jeong‐,Chae Wiley Subscription Services, Inc., A Wiley Company 2012 Journal of cellular biochemistry Vol.113 No.4
<P><B>Abstract</B></P><P>Numerous studies have shown that hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>) inhibits proliferation and osteoblastic differentiation in bone‐like cells. Human periodontal ligament fibroblasts (PLF) are capable of differentiating into osteoblasts and are exposed to oxidative stress during periodontal inflammation. However, the cellular responses of PLF to H<SUB>2</SUB>O<SUB>2</SUB> have not been identified. In this study, we examined how H<SUB>2</SUB>O<SUB>2</SUB> affects the viability and proliferation of PLF by exposing the cells to glucose oxidase (GO) or direct addition of H<SUB>2</SUB>O<SUB>2</SUB>. We also explored the effects of GO on the osteoblastic differentiation of PLF and the mechanisms involved. The viability and proliferation in PLF were increased with the addition of 10 mU/ml GO but not by volumes greater than 15 mU/ml or by H<SUB>2</SUB>O<SUB>2</SUB> itself. GO‐stimulated DNA synthesis was correlated with the increase in cyclin E protein levels in the cells. Osteoblastic differentiation of PLF was also augmented by combined treatment with GO, as evidenced by the increases in alkaline phosphatase activity, mineralization, collagen synthesis, and osteocalcin content in the cells. The inductions of runt‐related transcription factor 2 and osterix mRNA and proteins were further increased in PLF incubated in combination with GO compared to those in untreated cells. These results demonstrate that the continuous presence of H<SUB>2</SUB>O<SUB>2</SUB> stimulates the proliferation of PLF and augments their potential to differentiate into osteoblasts through the up‐regulation of bone‐specific transcription factors. Collectively, we suggest that H<SUB>2</SUB>O<SUB>2</SUB> may elicit the functions of PLF in maintaining the dimensions of the periodontal ligament and in mediating a balanced metabolism in alveolar bone. J. Cell. Biochem. 113: 1426–1436, 2012. © 2011 Wiley Periodicals, Inc.</P>
Three-Dimensional Graphene/MnO2 Nanowalls Hybrid for High-Efficiency Electrochemical Supercapacitors
Chuanyin Xiong,Tiehu Li,Tingkai Zhao,Alei Dang,Xianglin Ji,Hao Li,Mohammad Etesami 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.1
In this paper, a facile method is designed to fabricate three-dimensional (3D) graphene (GR)/ manganese dioxide (MnO2) nanowall electrode material. The 3D GR/MnO2 hybrid is prepared by a combination of electrochemical deposition (ELD) and electrophoresis deposition (EPD), followed by thermal reduction (TR). Firstly, the 3D graphene oxide (GO)/MnO2 hybrid is obtained by the ELD–EPD method. Secondly, the 3D GR/MnO2 hybrid is obtained through hydrogen reduction at a certain temperature. The as-fabricated hybrid has been characterized by scanning electron microscope (SEM), transmission electron microscope (TEM) and Raman spectroscopy. The electrochemical properties have been also measured by cyclic voltammetry. The results showed that the 3D GR/MnO2 nanowalls hybrid has a high specific capacitance of 266.75 Fg -1 and a high energy density of 25.36 Whkg -1. Moreover, a high specific capacitance (240.15 Fg -1) at a high scan rate of 200 mVs -1 (90% capacity retention) has been also obtained. Additionally, the hybrid can serve directly as the electrodes of supercapacitor without adding binder. This work provides a novel road to fabricate a binder-free 3D GR-based hybrid for high performance energy storage devices.