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        Removal of Uranium from Uranium Plant Wastewater Using Zero-Valent Iron in an Ultrasonic Field

        Jing Li,Libo Zhang,Jinhui Peng,Jinming Hu,Lifeng Yang,Aiyuan Ma,Hongying Xia,Wenqian Guo,Xia Yu 한국원자력학회 2016 Nuclear Engineering and Technology Vol.48 No.3

        Uranium removal from uranium plant wastewater using zero-valent iron in an ultrasonicfield was investigated. Batch experiments designed by the response surface methodology(RSM) were conducted to study the effects of pH, ultrasonic reaction time, and dosage ofzero-valent iron on uranium removal efficiency. From the experimental data obtained inthis work, it was found that the ultrasonic method employing zero-valent iron powdereffectively removes uranium from uranium plant wastewater with a uranium concentrationof 2,772.23 mg/L. The pH ranges widely from 3 to 7 in the ultrasonic field, and theprediction model obtained by the RSM has good agreement with the experimental results.

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        Transcriptome sequencing and characterization of Astragalus membranaceus var. mongholicus root reveals key genes involved in flavonoids biosynthesis

        Jianping Liang,Wenqian Li,Xiaoyun Jia,Ying Zhang,Jianping Zhao 한국유전학회 2020 Genes & Genomics Vol.42 No.8

        Background Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao is a traditional medicinal herb of Leguminosae since it contains bioactive compounds such as flavonoids, which have significant pharmacological effects on immunity and antioxidant. However, the scanty genomic and transcriptome resources of Astragalus membranaceus have hindered further exploration of its biosynthesis and accumulation mechanism. Objective This project aim to further improve our understanding of the relationship between transcriptional behavior and flavonoids content of A. mongholicus. Methods The accumulation of flavonoids and related gene expression in five different developmental stages (A: vegetative, B: florescence, C: fruiting, D: fruit ripening and E: defoliating stages) of A. mongholicus root were studied by combining UV spectrophotometry and transcriptomic techniques. The de novo assembly, annotation and functional evaluation of the contigs were performed with bioinformatics tools. Results After screening and assembling the raw data, there were a total of 158,123 unigenes with an average length of 644.89 bp were finally obtained, which has 8362 unigenes could be jointly annotated by NR, SwissProt, eggNOG, GO, KEGG and Pfam databases. KEGG enrichment analysis was performed on differentially expressed genes(DEGs)in the four groups (A vs. B, B vs. C, C vs. D, D vs. E). The results showed that many DEGs in each group were significantly enriched to flavonoids biosynthesis related pathways. Among them, a number of 86 were involved in the biosynthesis of isoflavonoid (12), flavonoid (5) and phenylpropanoid (69). Further analysis of these DEGs revealed that the expression levels of key genes such as PAL, 4CL, CCR , COMT, DFR, etc. were all down-regulated at the fruiting stage, and then raised at the fruit ripening stage. This expression pattern was similar to the accumulation trend of total flavonoids content. Conclusions In summary, this comprehensive transcriptome dataset allowed the identification of genes associated with flavonoids metabolic pathways. The results laid a foundation for the biosynthesis and regulation of flavonoids. It also provided a scientific basis for the most suitable harvest time and resource utilization of A. mongholicus.

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        High Precision and Low Force Resonant Probe Based on Quartz Tuning Fork

        Qiangxian Huang,Wenqian Wang,Guangpu Wang,Rongjun Cheng,Liansheng Zhang,Hongli Li,Ruijun Li 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.24 No.11

        To realize the high-precision three-dimensional (3D) measurement of micro-precision devices, a 3D resonant trigger probe based on quartz tuning fork for micro/nano coordinate measuring machine (CMM) is proposed. The probe is composed of a quartz tuning fork, a tapered optical fiber stylus and a microsphere. It vibrates in resonance state and makes contact with the measured surface in the Z direction in tapping mode, while in the X and Y directions, it operates in friction mode. The 3D nano-positioning of the probe is achieved by the changes in resonance parameter caused by the interatomic force between the microsphere and the surface of the measured sample. In this study, the diameter of the probe microsphere can be as low as 80 μm, and the length of the probe stylus is approximately 5 mm. The trigger resolution of the probe in the X, Y and Z directions are 0.44, 0.41 and 0.34 nm, respectively. The probing forces in the X, Y and Z directions are 2.25, 1.81 and 4.24 μN, respectively. Experimental results verify that the proposed probe has the advantages of small size, sub-nano resolution and very low probing force. This probe can be used as the trigger probe of micro/nano CMM, which can be triggered by interatomic force.

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