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Xufang Shen,Hongwei Yan,Lei Zhang,Zhen Yuan,Wenlei Liu,Yumeng Wu,Qi Liu,Xiaoyi Luo,Ying Liu 한국유전학회 2020 Genes & Genomics Vol.42 No.4
Background Quantification of mRNAs in gonads and other tissues at the early critical development stage of sex differentiation may help to provide a global view of regulatory mechanisms underlying sex differentiation. We have recently reported the transcriptomic profiling of fugu gonad associated with sex differentiation. Objectives This study attempted to identify the genes in the brain that are involved in gonadal differentiation and development. Methods In this study, a transcriptomic scan of potential candidate genes involved in sex differentiation was conducted in the brains of fugu larvae at 30 and 40 dah (morphological gonadal sex differentiation had not yet occurred). The dimorphic expression patterns of several candidate genes were verified using quantitative PCR. Results A total of 28.24 Gb of clean reads were obtained and 22,337 genes were identified in the brains of fugu larvae. These included 1008 novel genes that provide abundant data for functional analysis of sex differentiation. 229 genes were identified in the 30 dah larvae that were abundant in the XY brain and 21 that were abundant in the XX brain. In the 40 dah larvae, 325 genes were identified abundant in the XY brain and 174 were identified abundant in the XX brain. Conclusion This is the first investigation into the transcriptome of the fugu larvae brain at the early sex differentiation stage. The results obtained here will enhance the understanding of molecular mechanisms that underly fugu sex differentiation.
Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse
Bao, Wenlei,Yin, Jianxin,Liang, Yan,Guo, Zhixin,Wang, Yanfeng,Liu, Dongjun,Wang, Xiao,Wang, Zhigang Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.9
To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression of recombinant $6{\times}his-gVEGF164$ protein was induced by 0.5 mM isopropyl thio-${\beta}$-D-galactoside at $32^{\circ}C$. Recombinant goat VEGF164 (rgVEGF164) was purified and identified by western blot using monoclonal anti-his and anti-VEGF antibodies. The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group. Thus, rgVEGF164 increases hair growth in mice.
OSCILLATION OF NONLINEAR EQUATIONS ON TIME SCALES
Li, Qiaoluan,Liu, Zhiyong,Dong, Wenlei,Liang, Haiyan,Zhang, Zhenguo The Korean Society for Computational and Applied M 2009 Journal of applied mathematics & informatics Vol.27 No.1
By means of Riccati transformation techniques, we obtain some criteria which ensure that every solution of a nonlinear equation on time scales oscillates.
Oscillation of nonlinear equations on time scales
Qiaoluan Li,Zhiyong Liu,Wenlei Dong,Haiyan Liang,Zhenguo Zhang 한국전산응용수학회 2009 Journal of applied mathematics & informatics Vol.27 No.1
By means of Riccati transformation techniques, we obtain some criteria which ensure that every solution of a nonlinear equation on time scales oscillates. By means of Riccati transformation techniques, we obtain some criteria which ensure that every solution of a nonlinear equation on time scales oscillates.