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Claudio Pagano,Marco Venturi,Guido Benegiamo,Ernesto Melada,Contardo Vergani 대한외과학회 2020 Annals of Surgical Treatment and Research(ASRT) Vol.98 No.5
Purpose: Treatment of rectocele associated with prolapsed hemorrhoids is a debated topic. Transanal stapling achieved good midterm results in patients with symptoms of obstructed defecation, nevertheless a number of severe complications have been reported. The aim of this study was to evaluate the safety and efficacy of a new endorectal manual technique in patients with obstructed defecation due to the combination of muco-hemorrhoidal prolapse and rectocele. Methods: Patients enrolled after preoperative obstructed defecation syndrome (ODS) score, defecography and anoscopy were submitted to the novel Mucopexy-Recto Anal Lifting (MuRAL) combined with a modified Block procedure, and followed up by independent observers with digital exploration 3 weeks postoperatively, and digital exploration plus anoscopy at 3, 6, and 12 months. Operative time, hospital stay, numerating rating scale (NRS), ODS, satisfaction scores, and recurrence rate were recorded. Results: Mean operative time was 35.7 minutes. Fifty-six patients completed 1-year follow-up: 7.1% had acute urinary retention, NRS score was < 3 from the third postoperative day, mean time of daily activity resumption was 12 days, none had persistent fecal urgency, 82% declared excellent/good satisfaction score, significant improvement of 6- and 12-month ODS score, no recurrence of rectocele, and 7.1% recurrence of prolapsed hemorrhoids were observed. Conclusion: MuRAL associated with modified Block technique gave no severe complications and resulted in a safe and effective approach to symptomatic rectocele associated with muco-rectal prolapse. Further randomized studies, larger series, and longer follow-up are needed.
Mahmoudi, Esmaeil,Tabatabaei, Badraldin Ebrahim Sayed,Venturi, Vittorio The Korean Society of Plant Pathology 2011 Plant Pathology Journal Vol.27 No.3
Several soil bacteria were found to degrade N-Acylhomoserine lactones (NAHLs), thereby interfering with the bacterial quorum sensing system. In this research, fifteen strains of NAHL degrading rhizobacteria were isolated from potato rhizosphere. Based on phenotypic characteristics and 16S rDNA sequence analyses, the strains were identified as members of genera Bacillus, Streptomyces, Arthrobacter, Pseudomonas and Mesorhizobium. All tested isolates were capable to degrade both synthetic and natural NAHL produced by Pectobacterium carotovorum subsp. carotovorum (Pcc) strain EMPCC. In quorum quenching experiments selected isolates, especially Mesorhizobium sp., were markedly reduced the pathogenicity of Pcc strain EMPCC in potato tubers and totally suppressed tissue maceration on potato tubers. These led to consider the latter as a useful biocontrol agent against Pectobacterium spp.
HEATED INTRACLUSTER GAS AND RADIO CONNECTIONS: THE SINGULAR CASE OF MKW 3S
MAZZOTTA PASQUALE,BRUNETTI GIANFRANCO,GIACINTUCCI SIMONA,VENTURI TIZIANA,BARDELLI SANDRO The Korean Astronomical Society 2004 Journal of The Korean Astronomical Society Vol.37 No.5
Similarly to other cluster of galaxies previously classified as cooling flow systems, the Chandra observation of MKW 3s reveals that this object has a complex X-ray structure hosting both a X-ray cavity and a X-ray filament. Unlike the other clusters, however, the temperature map of the core of MKW 3s shows the presence of extended regions of gas heated above the radially averaged gas temperature at any radius. As the cluster does not show evidences for ongoing major mergers Mazzotta et al. suggest a connection between the heated gas and the activity of the central AGN. Nevertheless, due to the lack of high quality radio maps, this interpretation was controversial. In this paper we present the results of two new radio observations of MKW 3s at 1.28 GHz and 604 MHz obtained at the GMRT. Together with the Chandra observation and a separate VLA observation at 327 MHz from Young, we show unequivocal evidences for a close connection between the heated gas region and the AGN activity and we briefly summarize possible implications.
Branko Jovcic,Iris Bertani,Vittorio Venturi,Ljubisa Topisirovic,Milan Kojic 한국미생물학회 2008 The journal of microbiology Vol.46 No.1
The σS subunit of RNA polymerase is a central regulator which governs the expression of a host of stationary phase-induced and osmotically regulated genes in Gram-negative bacteria. The Pseudomonas putida rpoS gene is transcribed as a monocistronic rpoS mRNA with a 368 nucleotide-long 5’untranslated region (5’UTR). In this study, we investigate the posttranscriptional control of RpoS synthesis using rpoS-lacZ transcriptional and translational fusions consisting of the native promoter and deletions of 5’ UTR or insertion into UTR. The differing activity of constructed translational fusions strongly indicated that the 5’ UTR is involved in the translational regulation of RpoS expression in the stationary phase. The results obtained herein demonstrated that the structure of UTR performs an important function in the translational regulation of the rpoS gene.
Esmaeil Mahmoudi,Badraldin Ebrahim Sayed Tabatabaei,Vittorio Venturi 한국식물병리학회 2011 Plant Pathology Journal Vol.27 No.3
Several soil bacteria were found to degrade N-Acylhomoserine lactones (NAHLs), thereby interfering with the bacterial quorum sensing system. In this research,fifteen strains of NAHL degrading rhizobacteria were isolated from potato rhizosphere. Based on phenotypic characteristics and 16S rDNA sequence analyses, the strains were identified as members of genera Bacillus,Streptomyces, Arthrobacter, Pseudomonas and Mesorhizobium. All tested isolates were capable to degrade both synthetic and natural NAHL produced by Pectobacterium carotovorum subsp. carotovorum (Pcc) strain EMPCC. In quorum quenching experiments selected isolates, especially Mesorhizobium sp., were markedly reduced the pathogenicity of Pcc strain EMPCC in potato tubers and totally suppressed tissue maceration on potato tubers. These led to consider the latter as a useful biocontrol agent against Pectobacterium spp.
Devescovi, Giulia,Bigirimana, Joseph,Degrassi, Giuliano,Cabrio, Laura,LiPuma, John J.,Kim, Jinwoo,Hwang, Ingyu,Venturi, Vittorio American Society for Microbiology 2007 Applied and environmental microbiology Vol.73 No.15
<B>ABSTRACT</B><P><I>Burkholderia glumae</I> is an emerging rice pathogen in several areas around the world. Closely related <I>Burkholderia</I> species are important opportunistic human pathogens for specific groups of patients, such as patients with cystic fibrosis and patients with chronic granulomatous disease. Here we report that the first clinical isolate of <I>B. glumae</I>, strain AU6208, has retained its capability to be very pathogenic to rice. As previously reported for rice isolate <I>B. glumae</I> BGR1 (and also for the clinical isolate AU6208), TofI or TofR acyl homoserine lactone (AHL) quorum sensing played a pivotal role in rice virulence. We report that AHL quorum sensing in <I>B. glumae</I> AU6208 regulates secreted LipA lipase and toxoflavin, the phytotoxin produced by <I>B. glumae. B. glumae</I> AU6208 <I>lipA</I> mutants were no longer pathogenic to rice, indicating that the lipase is an important virulence factor. It was also established that type strain <I>B. glumae</I> ATCC 33617 did not produce toxoflavin and lipase and was nonpathogenic to rice. It was determined that in strain ATCC 33617 the LuxR family quorum-sensing sensor/regulator TofR was inactive. Introducing the <I>tofR</I> gene of <I>B. glumae</I> AU6208 in strain ATCC 33617 restored its ability to produce toxoflavin and the LipA lipase. This study extends the role of AHL quorum sensing in rice pathogenicity through the regulation of a lipase which was demonstrated to be a virulence factor. It is the first report of a clinical <I>B. glumae</I> isolate retaining strong rice pathogenicity and finally determined that <I>B. glumae</I> can undergo phenotypic conversion through a spontaneous mutation in the <I>tofR</I> regulator.</P>
Thu Thi Hieu Nguyen,Cristina Bez,Iris Bertani,Minh Hong Nguyen,Thao Kim Nu Nguyen,Vittorio Venturi,Hang Thuy Dinh 한국식물병리학회 2024 Plant Pathology Journal Vol.40 No.1
The microbiomes of two important rice cultivars in Vietnam which differ by their susceptibility to the bacterial leaf blight (BLB) disease were analyzed through 16S rRNA amplicon technology. A higher number of operational taxonomic units and alpha-diversity indices were shown in the BLB-resistant LA cultivar than in the BLB-susceptible TB cultivar. The BLB pathogen Xanthomonas was scantly found (0.003%) in the LA cultivar, whereas was in a significantly higher ratio in the TB cultivar (1.82%), reflecting the susceptibility to BLB of these cultivars. Of special interest was the genus Acholeplasma presented in the BLB-resistant LA cultivar at a high relative abundance (22.32%), however, was minor in the BLB-sensitive TB cultivar (0.09%), raising a question about its roles in controlling the Xanthomonas low in the LA cultivar. It is proposed that Acholeplasma once entered the host plant would hamper other phytopathogens, i.e. Xanthomonas, by yet unknown mechanisms, of which the triggering of the host plants to produce secondary metabolites against pathogens could be a testable hypothesis.