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Seshagirirao, Kottapalli,Leelavathi, Chaganti,Sasidhar, Vemula Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.3
A cross-linked leucaena (Leucaena leucocephala) seed gum (CLLSG) matrix was prepared for the isolation of galactose-specific lectins by affinity chromatography. The matrix was evaluated for affinity with a known galactose-specific lectin from the seeds of snake gourd (Trichosanthes anguina). The matrix preparation was simple and inexpensive when compared to commercial galactose-specific matrices (i.e. about 1.5 US$/100 ml of matrix). The current method is also useful for the demonstration of the affinity chromatography technique in laboratories. Since leucaena seeds are abundant and inexpensive, and the matrix preparation is easy, CLLSG appears to be a promising tool for the separation of galactose-specific lectins.
Prasain, Nutan,Lee, Man Ryul,Vemula, Sasidhar,Meador, Jonathan Luke,Yoshimoto, Momoko,Ferkowicz, Michael J,Fett, Alexa,Gupta, Manav,Rapp, Brian M,Saadatzadeh, Mohammad Reza,Ginsberg, Michael,Elemento, Nature Publishing Group, a division of Macmillan P 2014 Nature biotechnology Vol.32 No.11
The ability to differentiate human pluripotent stem cells into endothelial cells with properties of cord-blood endothelial colony–forming cells (CB-ECFCs) may enable the derivation of clinically relevant numbers of highly proliferative blood vessel–forming cells to restore endothelial function in patients with vascular disease. We describe a protocol to convert human induced pluripotent stem cells (hiPSCs) or embryonic stem cells (hESCs) into cells similar to CB-ECFCs at an efficiency of >10<SUP>8</SUP> ECFCs produced from each starting pluripotent stem cell. The CB-ECFC-like cells display a stable endothelial phenotype with high clonal proliferative potential and the capacity to form human vessels in mice and to repair the ischemic mouse retina and limb, and they lack teratoma formation potential. We identify Neuropilin-1 (NRP-1)-mediated activation of KDR signaling through VEGF<SUB>165</SUB> as a critical mechanism for the emergence and maintenance of CB-ECFC-like cells.