<i>Epilithonimonas ginsengisoli</i> sp. nov., isolated from soil of a ginseng field

Hoang, Van-An,Kim, Yeon-Ju,Ponnuraj, Shree Priya,Nguyen, Ngoc-Lan,Hwang, Kyu-Hyon,Yang, Deok-Chun International Union of Microbiological Societies 2015 International journal of systematic and evolutiona Vol.65 No.1

<P>A novel Gram-staining-negative, rod-shaped bacterium, designated DCY78<SUP>T</SUP>, was isolated from soil of a ginseng field in Yeon-cheon province (38° 04′ 00″ N 126° 57′ 00″ E), Republic of Korea. The phylogenetic analysis based on 16S rRNA gene sequences showed that strain DCY78<SUP>T</SUP> belonged to the genus <I>Epilithonimonas</I> and was most closely related to <I>Epilithonimonas lactis</I> DSM 19921<SUP>T</SUP> (98.5 % sequence similarity) and <I>Epilithonimonas tenax</I> DSM 16811<SUP>T</SUP> (97.8 %). Growth occurred at 10–30 °C with an optimum temperature of 28 °C. The pH range for growth was pH 5.5–8.0. The major polar lipids were found to be phosphatidylethanolamine three unidentified amino lipids and one unidentified polar lipid. The only predominant quinone was MK-6. The major polyamines were <I>sym</I>-homospermidine and spermidine. The major fatty acids were summed feature 3 (comprising C<SUB>16 : 1</SUB>ω6<I>c</I> and/or C<SUB>16 : 1</SUB>ω7<I>c</I>), iso-C<SUB>15 : 0</SUB> and iso-C<SUB>17 : 0</SUB> 3-OH. The DNA G+C content was 37.9 mol%. On the basis of the phenotypic and genotypic analysis, the isolate is classified as representative of a novel species in the genus <I>Epilithonimonas</I>, for which the name <I>Epilithonimonas</I> <I>ginsengisoli</I> is proposed. The type strain is DCY78<SUP>T</SUP> ( = KCTC 32174<SUP>T</SUP> = JCM 19896<SUP>T</SUP>).</P>

<i>Arthrobacter gyeryongensis</i> sp. nov., isolated from soil of a <i>Gynostemma pentaphyllum</i> field

Hoang, Van-An,Kim, Yeon-Ju,Nguyen, Ngoc-Lan,Yang, Deok-Chun International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.2

<P>A Gram-stain-positive, flagellate, rod-shaped, catalase- and oxidase-positive bacterium, designated DCY72<SUP>T</SUP>, was isolated from the soil of a <I>Gynostemma pentaphyllum</I> field. Growth occurred at 4–34 °C (optimum 30 °C), at pH 4–10 (optimum pH 7), and with 0–5 % NaCl (w/v). The major menaquinones of strain DCY72<SUP>T</SUP> were MK-9(H<SUB>2</SUB>) (81.0 %) and MK-10(H<SUB>2</SUB>) (12.2 %). The major amino acid present in the cell-wall peptidoglycan was <SMALL>l</SMALL>-lysine. The major fatty acids were anteiso-C<SUB>15 : 0</SUB> and anteiso-C<SUB>17 : 0</SUB>. The genomic DNA G+C content was 64.5 mol%. 16S rRNA gene sequence analysis revealed that strain DCY72<SUP>T</SUP> belonged to the family <I>Micrococcaceae</I> and was most closely related to <I>Arthrobacter ramosus</I> CCM 1646<SUP>T</SUP> (98.2 % similarity). The DNA–DNA relatedness between strain DCY72<SUP>T</SUP> and <I>A. ramosus</I> KACC 14391<SUP>T</SUP> (98.2 % 16S rRNA gene sequence similarity), <I>Arthrobacter nitroguajacolicus</I> KACC 14581<SUP>T</SUP> (97.6 %), <I>Arthrobacter nicotinovorans</I> KACC 20508<SUP>T</SUP> (97.3 %) and <I>Arthrobacter aurescens</I> KACC 20528<SUP>T</SUP> (97.3 %). was 12.9 %±0.3, 25.6 %±0.3, 26.6 %±0.5 and 23.2 %±0.9, respectively. On the basis of the phenotypic characteristics, genotypic analysis and physiological characteristics, strain DCY72<SUP>T</SUP> represents a novel species of the genus <I>Arthrobacter</I>, for which the name <I>Arthrobacter gyeryo</I><I>ngensis</I> sp. nov. is proposed. The type strain is DCY72<SUP>T</SUP> ( = KCTC 33072<SUP>T</SUP> = JCM 18514<SUP>T</SUP>).</P>

Hymenobacter ginsengisoli sp. nov., isolated from soil of a ginseng field.

Hoang, Van-An,Kim, Yeon-Ju,Nguyen, Ngoc Lan,Yang, Deok-Chun Society for General Microbiology 2013 International journal of systematic and evolutiona Vol.63 No.2

<P>A Gram-stain-negative, non-motile, red bacterium, designated DCY57(T), was isolated from soil of a ginseng field in a mountainous region of Chungnam province in South Korea. Strain DCY57(T) grew with 0-1?% (w/v) NaCl and the optimum temperature for growth was 30 C. Strain DCY57(T) contained MK-7 as the predominant menaquinone. The polyamine was sym-homospermidine. The major fatty acids were C(16:1)ω5c, iso-C(15:0), anteiso-C(15:0) and summed feature 3 (containing C(16:1)ω7c and/or C(16:1)ω6c). The major polar lipids were phosphatidylethanolamine, unknown aminophospholipids, unknown aminolipids and unknown lipids. The DNA G+C content was 58.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DCY57(T) was most closely related to members of the genus Hymenobacter. The isolate exhibited 91.7?% 16S rRNA gene sequence similarity with H. soli PB17(T), 94.5?% with H. flocculans A2-50A(T) and 95.8?% with H. metalli A2-91(T). On the basis of the evidence presented in this study, strain DCY57(T) represents a novel species within the genus Hymenobacter, for which the name Hymenobacter ginsengisoli sp. nov. is proposed. The type strain is DCY57(T) (?=?KCTC 23674(T)?=?JCM 17841(T)).</P>

Chryseobacterium ginsengiterrae sp. nov., a bacterium with ginsenoside converting activity isolated from soil of a ginseng field

Van-An Hoang,Yeon-Ju Kim,Ngoc-Lan Nguyen,Ji Na Joen,Jin-Woo Min,Yan Jin,Deok-Chun Yang 한국약용작물학회 2012 한국약용작물학회 학술대회논문집 Vol.2012 No.1

<i>Brachybacterium ginsengisoli</i> sp. nov., isolated from soil of a ginseng field

Hoang, Van-An,Kim, Yeon-Ju,Nguyen, Ngoc-Lan,Yang, Deok-Chun International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.9

<P>A novel Gram-staining-positive, aerobic bacterium, designed DCY80<SUP>T</SUP>, was isolated from soil of a ginseng field in the Republic of Korea. 16S rRNA gene sequence analysis revealed that strain DCY80<SUP>T</SUP> belonged to the genus <I>Brachybacterium</I> (95.8–98.2 % similarity) and was most closely related to <I>Brachybacterium faecium</I> DSM 4810<SUP>T</SUP> (98.2 %). Colonies were circular, entire, low-convex, opaque and 0.5–1.0 mm in diameter after growth for 2 days on TSA at 30 °C. Growth occurred at 4–34 °C (optimum, 25 °C), at pH 5.0–10.0 (optimum, pH 6.5–7.0) and in the presence of 0–7.0 % NaCl. Strain DCY80<SUP>T</SUP> produced siderophores and was sensitive to penicillin G, erythromycin, cefazolin, oleandomycin, ceftazidime, vancomycin, tetracycline, novobiocin, carbamicillin, rifampicin and neomycin. The DNA G+C content was 71.0 mol%. Levels of DNA–DNA relatedness between strain DCY80<SUP>T</SUP> and <I>B. faecium</I> DSM 4810<SUP>T</SUP>, <I>B. paraconglomeratum</I> KCTC 9916<SUP>T</SUP>, <I>B. saurashtrense</I> DSM 23186<SUP>T</SUP> and <I>B. conglomeratum</I> KCTC 9915<SUP>T</SUP> were 46.9±0.5, 28.9±0.6, 20.4±0.9 and 17.3±0.4 %, respectively. The cell-wall peptidoglycan of strain DCY80<SUP>T</SUP> contained <I>meso</I>-diaminopimelic acid as the diagnostic diamino acid. The menaquinones were MK-7 (85.8 %) and MK-8 (14.2 %). The major cellular fatty acids were anteiso-C<SUB>15 : 0</SUB> (69.1 %) and anteiso-C<SUB>17 : 0</SUB> (12.2 %). Phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid, two unidentified phospholipids and five unidentified polar lipids were found. On the basis of our phenotypic and genotypic analyses, strain DCY80<SUP>T</SUP> represents a novel species of the genus <I>Brachybacterium</I>, for which the name <I>Brachybacterium ginsengisoli</I> sp. nov. is proposed (type strain DCY80<SUP>T</SUP> = KCTC 29226<SUP>T</SUP> = JCM 19356<SUP>T</SUP>).</P>

Chryseobacterium yeoncheonense sp. nov., with ginsenoside converting activity isolated from soil of a ginseng field

Hoang, Van-An,Kim, Yeon-Ju,Nguyen, Ngoc Lan,Yang, Deok-Chun Springer-Verlag 2013 Archives of microbiology Vol.195 No.7

<P>A Gram-staining negative, aerobic, non-motile, non-flagellate, yellow-pigmented, rod-shaped bacterial strain, designated strain DCY67(T), was isolated from ginseng field in Republic of Korea. Strain DCY67(T) contained β-glucosidase activity which converts ginsenoside Rb1 to compound K. Optimum growth of DCY67(T) occurred at 30?C and pH 6.0-6.5. Analysis of the 16S rRNA gene sequences revealed that strain DCY67(T) belonged to the family Flavobacteriaceae and was most closely related to Chryseobacterium ginsenosidimutans THG 15(T) (97.5?%). The genomic DNA G+C content was 36.1?mol%. The predominant quinones were MK-6 (90.9?%) and MK-7 (9.15?%). The major fatty acids were iso-C15:0, summed feature 3 (containing C16:1 ω7c and/or C16:1 ω6c) and iso-C17:0 3-OH. On the basis of these phenotypic, genotypic and chemotaxonomic studies, strain DCY67(T) represents a novel species of the genus Chryseobacterium, for which, name Chryseobacterium yeoncheonense sp. nov. proposed the type strain is DCY67(T) (=KCTC 32090(T)?=?JCM 18516(T)).</P>

<i>Pedobacter ginsengiterrae</i> sp. nov., isolated from soil of a ginseng field

Hoang, Van-An,Kim, Yeon-Ju,Nguyen, Ngoc-Lan,Min, Jin-Woo,Yang, Deok-Chun International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.4

<P>A Gram-stain-negative, oxidase- and catalase-positive bacterial strain that was motile by gliding and produced a pink pigment, designated DCY49(T), was isolated from soil of a ginseng field in a mountainous region of Chungbuk province, South Korea. 16S rRNA gene sequence analysis revealed that strain DCY49(T) belonged to the genus Pedobacter (93.0-96.3 % similarity). Strain DCY49(T) contained MK-7 as the predominant menaquinone. The major fatty acids were summed feature 3 (containing C-16:1 omega 7C, C-16:1 omega 6c and/or iso-C-15:0 2-OH), iso-C-15:0, iso-C-17:0 3-OH and C-16:0, and the main polar lipid was phosphatidylethanolamine. The G+C content of the genomic DNA of strain DCY49(T) was 40.5 mol%. Strain DCY49(T) differed from related Pedobacter species by a number of phenotypic characteristics. On the basis of data from the present polyphasic study, strain DCY49(T) is described as representing a novel species of the genus Pedobacter, for which the name Pedobacter ginsengiterrae sp. nov. is proposed. The type strain is DCY49(T) (=KCTC 23317(T)=JCM 17338(T)).</P>

Healthcare Workers’ Knowledge and Attitudes Regarding the World Health Organization’s “My 5 Moments for Hand Hygiene”: Evidence From a Vietnamese Central General Hospital

Huy Van Nguyen,Hieu Trung Tran,Long Quynh Khuong,Thanh Van Nguyen,Na Thi Nhi Ho,An Thi Minh Dao,Minh Van Hoang 대한예방의학회 2020 예방의학회지 Vol.53 No.4

Objectives: Although the World Health Organization (WHO) initiative “My 5 Moments for Hand Hygiene” has been lauded as effective in preventing hospital-associated infections, little is known about healthcare workers (HCWs)’ hand hygiene behavior. In this study, we sought to assess knowledge and attitudes towards the concepts in this initiative, as well as associated factors, among Vietnamese HCWs at a general hospital. Methods: A structured questionnaire was administered to HCWs at a central Vietnamese general hospital in 2015. Multiple logistic regression analysis was used to identify factors associated with HCWs’ knowledge and attitudes towards hand hygiene. Results: Of 120 respondents, 65.8% and 67.5% demonstrated appropriate knowledge and a positive attitude, respectively, regarding all 5 hand hygiene moments. Logistic regression indicated better knowledge of hand hygiene in workers who were over 30 years old, who were direct HCWs (rather than managers), who had frequent access to clinical information, and who received their clinical information from training. Those who worked in infectious and tropical disease wards, who had frequent access to clinical information, and who received information from training were more likely to have a positive attitude towards hand hygiene than their counterparts. Conclusions: Although many Vietnamese HCWs displayed moderate knowledge and positive attitudes towards the WHO hand hygiene guidelines, a key gap remained. Regular education and training programs are needed to increase knowledge and to improve attitudes and practices towards hand hygiene. Furthermor

Paralcaligenes ginsengisoli sp. nov., isolated from ginseng cultivated soil

Kang, Jong-Pyo,Nguyen, Ngoc-Lan,Kim, Yeon-Ju,Hoang, Van-An,Bae, Kwi-Sik,Yang, Deok-Chun Springer-Verlag 2015 Antonie van Leeuwenhoek Vol.108 No.3

<P>A novel bacterial strain DCY104(T) isolated from soil of a ginseng field in Yeoncheon County, Republic of Korea is described in this study. Cells were short rod-shaped, motile by mean of one polar flagellum, strictly aerobic, Gramreaction negative, oxidase and catalase-positive. 16S rRNA gene sequence analysis showed that strain DCY104(T) shared highest similarity 98.2?% to Paralcaligenes ureilyticus GR24-5(T), and from 97.7 to 97.1?% with other type strains belong to the genera Candidimonas, Pusillimonas and Parapusillimonas Otherwise, phylogenetic trees analyses indicated that strain DCY104(T) belongs to a single group with P. ureilyticus GR24-5(T) that was distinct to other genera. The major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The major cellular fatty acids consisted of C16:0, cyclo-C17:0, and summed feature 8 (comprising C18:1 ω7c and/or C18:1 ω6c). The predominant polyamine was putrescine. The ubiquinone was Q-8. The genomic DNA G+C content was 55.9?mol%. These data in combination with the presence of one polar flagellum and positive activity of urease confirmed the placement of strain DCY104(T) in the genus Paralcaligenes. DNA-DNA relatedness between strain DCY104(T) and P. ureilyticus KACC 13888(T) was 40?%. The differences in the profiles of polar lipids, fatty acids and phenotypic characteristics in combination with DNA-DNA relatedness delineated strain DCY104(T) and P. ureilyticus KACC 13888(T). In summary, taxonomic analyses in this study demonstrated that strain DCY104(T) represents a novel species within the genus Paralcaligenes, for which we propose the name Paralcaligenes ginsengisoli. The type strain is DCY104(T) (=?KCTC 42406(T)?=?JCM 30746(T)).</P>

Sphingomonas panacis sp. nov., isolated from rhizosphere of rusty ginseng

Singh, Priyanka,Kim, Yeon-Ju,Hoang, Van-An,Farh, Mohamed El-Agamy,Yang, Deok-Chun Springer-Verlag 2015 Antonie van Leeuwenhoek Vol.108 No.3