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Nikitkina, Elena V.,Dementieva, Natalia V.,Shcherbakov, Yuri S.,Atroshchenko, Mikhail M.,Kudinov, Andrei A.,Samoylov, Oleg I.,Pozovnikova, Marina V.,Dysin, Artem P.,Krutikova, Anna A.,Musidray, Artem Asian Australasian Association of Animal Productio 2022 Animal Bioscience Vol.35 No.12
Objective: The semen quality of stallions including sperm motility is an important target of selection as it has a high level of individual variability. However, effects of the molecular architecture of the genome on the mechanisms of sperm formation and their preservation after thawing have been poorly investigated. Here, we conducted a genome-wide association study (GWAS) for the sperm motility of cryopreserved semen in stallions of various breeds. Methods: Semen samples were collected from the stallions of 23 horse breeds. The following semen characteristics were examined: progressive motility (PM), progressive motility after freezing (FPM), and the difference between PM and FPM. The respective DNA samples from these stallions were genotyped using Axiom Equine Genotyping Array. Results: We performed a GWAS search for single nucleotide polymorphism (SNP) markers and potential genes related to motility properties of frozen-thawed semen in the stallions of various breeds. As a result of the GWAS analysis, two SNP markers, rs1141327473 and rs1149048772, were identified that were associated with preservation of the frozen-thawed stallion sperm motility, the relevant putative candidate genes being NME/NM23 family member 8 (NME8), olfactory receptor family 2 subfamily AP member 1 (OR2AP1), and olfactory receptor family 6 subfamily C member 4 (OR6C4). Potential implications of effects of these genes on sperm motility are herein discussed. Conclusion: The GWAS results enabled us to localize novel SNPs and candidate genes for sperm motility in stallions. Implications of the study for horse breeding and genetics are a better understanding of genomic regions and candidate genes underlying stallion sperm quality, and improvement in horse reproduction and breeding techniques. The identified markers and genes for sperm cryotolerance and the respective genomic regions are promising candidates for further studying the biological processes in the formation and function of the stallion reproductive system.
HTS high gradient magnetic separator prototype
Diev, D.N.,Lepehin, V.M.,Makarenko, M.N.,Polyakov, A.V.,Shcherbakov, V.I.,Shutova, D.I.,Surin, M.I.,Tagunov, E. Ya. The Korean Society of Superconductivity and Cryoge 2018 한국초전도저온공학회논문지 Vol.20 No.4
A high gradient magnetic (HGM) separator prototype with the $2^{nd}$ generation high temperature superconducting (2G HTS) magnetic system operated in sub-cooled nitrogen is presently under development at NRC "Kurchatov Institute" (Moscow, Russia). The main goal of the project is an attempt to shift away from the complicated liquid helium cryostats towards simple cryocooler-based nitrogen cryogenics as much more convenient for HGM separators industrial applications. Using of commercial HTS tapes allows to get a sufficient level of magnetic fields and extraction forces with low energy consumption. The expected operational parameters of the device are 1.2-1.5 T in the empty operational gap and up to 3 T on the ferromagnetic filters. In this paper we briefly describe the design of the HTS rotary separator prototype with the horizontally oriented rotor axis and propose different types of ferromagnetic filters intended for weakly magnetic ores enrichment.
HTS high gradient magnetic separator prototype
D. N. Diev,V. M. Lepehin,M. N. Makarenko,A. V. Polyakov,V. I. Shcherbakov,D. I. Shutova,M. I. Surin,E. Ya. Tagunov 한국초전도.저온공학회 2018 한국초전도저온공학회논문지 Vol.20 No.4
A high gradient magnetic (HGM) separator prototype with the 2nd generation high temperature superconducting (2G HTS) magnetic system operated in sub-cooled nitrogen is presently under development at NRC “Kurchatov Institute” (Moscow, Russia). The main goal of the project is an attempt to shift away from the complicated liquid helium cryostats towards simple cryocooler-based nitrogen cryogenics as much more convenient for HGM separators industrial applications. Using of commercial HTS tapes allows to get a sufficient level of magnetic fields and extraction forces with low energy consumption. The expected operational parameters of the device are 1.2-1.5 T in the empty operational gap and up to 3 T on the ferromagnetic filters. In this paper we briefly describe the design of the HTS rotary separator prototype with the horizontally oriented rotor axis and propose different types of ferromagnetic filters intended for weakly magnetic ores enrichment.
REBCO coil operation in gaseous helium and solid nitrogen
D. N. Diev*,M. N. Makarenko,A. V. Naumov,A. V. Polyakov,V. I. Shcherbakov,D. I. Shutova,M. I. Surin 한국초전도.저온공학회 2019 한국초전도저온공학회논문지 Vol.21 No.3
The paper gives the results of the experiments with a model two-section REBCO solenoid cooled by either gaseous helium (GHe) or sub-cooled/solid nitrogen (SN2) in (50-77) K temperature range. The major cooling source was a single-stage cryocooler Sumitomo CH-110 with the cooling power of 175 W and 130 W at 77 K and 50 K respectively. The coil itself was not directly conduction cooled. We compare the time taken by both coolants to obtain the temperature of the magnet of about 50 K and the homogeneity of the temperature distribution within the cryostat. Test results for the coil operation in solid nitrogen together with the comparison of its critical properties in SN2 and GHe are also presented.
REBCO coil operation in gaseous helium and solid nitrogen
Diev, D.N.,Makarenko, M.N.,Naumov, A.V.,Polyakov, A.V.,Shcherbakov, V.I.,Shutova, D.I.,Surin, M.I. The Korea Institute of Applied Superconductivity a 2019 한국초전도저온공학회논문지 Vol.21 No.3
The paper gives the results of the experiments with a model two-section REBCO solenoid cooled by either gaseous helium (GHe) or sub-cooled/solid nitrogen (SN2) in (50-77) K temperature range. The major cooling source was a single-stage cryocooler Sumitomo CH-110 with the cooling power of 175 W and 130 W at 77 K and 50 K respectively. The coil itself was not directly conduction cooled. We compare the time taken by both coolants to obtain the temperature of the magnet of about 50 K and the homogeneity of the temperature distribution within the cryostat. Test results for the coil operation in solid nitrogen together with the comparison of its critical properties in SN2 and GHe are also presented.