Cyclometallated iridium complexes inducing paraptotic cell death like natural products: synthesis, structure and mechanistic aspects

Tripathy, Suman Kumar,De, Umasankar,Dehury, Niranjan,Laha, Paltan,Panda, Manas Kumar,Kim, Hyung Sik,Patra, Srikanta The Royal Society of Chemistry 2016 Dalton Transactions Vol.45 No.38

<P>Six mononuclear Ir complexes (1-6) using polypyridyl-pyrazine based ligands (L-1 and L-2) and {[cp*IrCl-(mu-Cl)](2) and [(ppy)(2)Ir(mu-Cl)](2)} precursors have been synthesised and characterised. Complexes 1-5 have shown potent anticancer activity against various human cancer cell lines (MCF-7, LNCap, Ishikawa, DU145, PC3 and SKOV3) while complex 6 is found to be inactive. Flow cytometry studies have established that cellular accumulation of the complexes lies in the order 2 > 1 > 5 > 4 > 3 > 6 which is in accordance with their observed cytotoxicity. No changes in the expression of the proteins like PARP, caspase 9 and beclin-1, Atg12 discard apoptosis and autophagy, respectively. Overexpression of CHOP, activation of MAPKs (P38, JNK, and ERK) and massive cytoplasmic vacuolisation collectively suggest a paraptotic mode of cell death induced by proteasomal dysfunction as well as endoplasmic reticulum and mitochondrial stress. An intimate relationship between p53, ROS production and extent of cell death has also been established using p53 wild, null and mutant type cancer cells.</P>

Dinuclear [{(<i>p</i>-cym)RuCl}₂(μ-phpy)](PF₆)₂ and heterodinuclear [(ppy)₂Ir(μ-phpy)Ru(<i>p</i>-cym)Cl](PF₆)₂ complexes: synthesis, structure and anticancer activity

Tripathy, Suman Kumar,De, Umasankar,Dehury, Niranjan,Pal, Satyanarayan,Kim, Hyung Sik,Patra, Srikanta The Royal Society of Chemistry 2014 Dalton Transactions Vol.43 No.39

<P>Phpy bridged homodinuclear Ru–Ru (<B>1</B>) and heterodinuclear Ir–Ru complexes (<B>2</B>) have been developed. Complex <B>2</B> induces autophagy towards the cisplatin resistant human breast cancer (MCF7) cell line, whereas <B>1</B> is inactive.</P> <P>Graphic Abstract</P><P>Heterodinuclear Ir–Ru (<B>2</B>) with polypyridyl based phpy ligand shows autophagy induced cell death, whereas homodinuclear Ru–Ru (<B>1</B>) is inactive. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c4dt01033g'> </P>

Synthesis, characterization and dielectric investigation of ZnO-doped polyaniline nanocomposites

Suman Lal,Surya Kant Tripathi,Rajesh Kumar,Nitin Sood,Samriti Khosla 한국정보디스플레이학회 2015 Journal of information display Vol.16 No.1

Polyaniline (PANI) synthesized through the in situ polymerization method and its composites with zinc oxide nanoparticles loaded in various weight percentages were studied. The formation of PANI and its composites was characterized using different characterizing tools, and detailed analysis was done to investigate the structural and morphological properties of the composites. The presence of the vibration band of the metal oxide and other characteristic bands of the composites, as characterized via Fourier transmission infrared spectroscopy, confirmed the synthesis of the polymer nanocomposites. The X-ray diffraction patterns of the nanocomposites revealed their polycrystalline nature. The dielectric responses of PANI and the ZnO-doped PANI composites were investigated within the frequency range of 20 Hz to 1 MHz. The dielectric constant of the ZnO-doped PANI composite was found to be higher than that of the pure PANI. The value of the dielectric constant decreased with increasing frequency, and it became constant at higher frequencies, which is attributed to the interfacialpolarization.

Intrinsic Vertebral Markers for Spinal Level Localization in Anterior Cervical Spine Surgery: A Preliminary Report

Deepak Kumar Jha,Anil Thakur,Mukul Jain,Arvind Arya,Chandrabhushan Tripathi,Rima Kumari,Suman Kushwaha 대한척추외과학회 2016 Asian Spine Journal Vol.10 No.6

Study Design: Prospective clinical study. Purpose: To observe the usefulness of anterior cervical osteophytes as intrinsic markers for spinal level localization (SLL) during sub-axial cervical spinal surgery via the anterior approach. Overview of Literature: Various landmarks, such as the mandibular angle, hyoid bone, thyroid cartilage, first cricoid ring, and C6 carotid tubercle, are used for gross cervical SLL; however, none are used during cervical spinal surgery via the anterior approach. We present our preliminary assessment of SLL over anterior vertebral surfaces (i.e., intrinsic markers) in 48 consecutive cases of anterior cervical spinal surgeries for the disc-osteophyte complex (DOC) in degenerative diseases and granulation or tumor tissue associated with infectious or neoplastic diseases, respectively, at an ill-equipped center. Methods: This prospective study on patients undergoing anterior cervical surgery for various sub-axial cervical spinal pathologies aimed to evaluate the feasibility and accuracy of SLL via intraoperative palpation of disease-related morphological changes on anterior vertebral surfaces visible on preoperative midline sagittal T1/2-weighted magnetic resonance images. Results: During a 3-year period, 48 patients (38 males,10 females; average age, 43.58 years) who underwent surgery via the anterior approach for various sub-axial cervical spinal pathologies, including degenerative disease (n= 42), tubercular infection (Pott’s disease; n=3), traumatic prolapsed disc (n=2), and a metastatic lesion from thyroid carcinoma (n=1), comprised the study group. Intrinsic marker palpation yielded accurate SLL in 79% of patients (n=38). Among those with degenerative diseases (n=42), intrinsic marker palpation yielded accurate SLL in 76% of patients (n=32). Conclusions: Intrinsic marker palpation is an attractive potential adjunct for SLL during cervical spinal surgeries via the anterior approach in well-selected patients at ill-equipped centers (e.g., those found in developing countries). This technique may prove helpful when radiographic visualization is occasionally inadequate.

A Journey from Bulk MOSFET to 3 nm and Beyond

Asharani Samal,Suman Lata Tripathi,Sushanta Kumar Mohapatra 한국전기전자재료학회 2020 Transactions on Electrical and Electronic Material Vol.21 No.5

To overcome scaling issues such as controlling gate leakage, drain induced barrier lowering, higher subthreshold conduction, polysilicon gate depletion, and other short channel eff ects various engineering proposed. The gate dielectric, metal work function, and device structural engineering enabled the semiconductor industry to make a transition from the conventional planar MOSFET towards a revolutionary 3D tri-gate structure called FinFET. FinFET is one of the fundamental invention in the semiconductor industry, which replaced the planar CMOS technology around 22 nm technology. By following Moore’s law, it accelerated the scaling to 7 nm, but at 5 nm, in the same way, GAAFET replaced FinFET due to technological hurdles. Nanosheet, which is one type of GAAFET are in the recent trend. But researchers are trying to explore the possibilities to continue the miniaturization beyond 3 nm by combining the eff ect of non-silicon channel material such as Ge, InGaAs, or 2D materials with nanosheet, which will improve the functionality of the device while going down in the technology node. In this survey, an attempt has been made for the structure present till 7 nm process. Also, a few new proposals in research to take the scaling up to 3 nm and beyond are included. The future innovations may put an intercept on the slowing down of Moore’s law, and bring the miniaturization back in the track.

A Critical Aspect of Bioreactor Designing and Its Application for the Generation of Tissue Engineered Construct: Emphasis on Clinical Translation of Bioreactor

Aditya Anand,Sarada Prasanna Mallick,Bhisham Narayan Singh,Shikha Kumari,Dheerendra Kumar Suman,Satyavrat Tripathi,Divakar Singh,Pradeep Srivastava 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.4

Usage of bioreactors in the field of tissue engineering has played a significant role in enabling a controlled and reproducible change in the formation of damaged tissue on being provided with specific factors. Owing to the scarcity seen in providing sufficient donor organs for transplantation there is a huge requirement for large-scale production of artificial organs. This cannot be achieved by static culturing since it does not provide an invivo three-dimensional (3D) microenvironment therefore tissue engineering plays a vital role in the development of artificial tissues and organs as per the clinical demands whereas bioreactors have served a major role in providing the artificial microenvironment required by the cells to grow further into a tissue and then into an organ. By providing the specific biochemical cues and mechanoresponsive stimuli the bioreactors turn to be very effective in generating transplantable organs. Apart from performing studies in a controlled manner aimed at understanding biological and physicochemical effects, bioreactors also ensure the safe and reproducible production of tissueengineered constructs to achieve cost-effective large-scale production. The design criteria for bioreactors to be used in tissue engineering include optimal aspect ratio, proper aeration for the cells to proliferate, and agitation with reduced shear stress. The current review summarizes important aspects like Height/Diameter ratio or aspect ratio, shear stress, mechanical stress, aeration, agitation, oxygenation, etc. related to the design of tissue bioreactors, different types of bioreactors that are in use to date, and the reported pieces of literature to yield an overview on the existing concepts. It mainly focuses on the generation of 3D tissue constructs in various reactor systems specially designed for their culture and development along with their applications.