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Chae, Tong Un,Ko, Yoo-Sung,Hwang, Kyu-Sang,Lee, Sang Yup Elsevier 2017 Metabolic engineering Vol.41 No.-
<P><B>Abstract</B></P> <P>Microbial production of chemicals and materials from renewable sources is becoming increasingly important for sustainable chemical industry. Here, we report construction of a new and efficient platform metabolic pathway for the production of four-carbon (butyrolactam), five-carbon (valerolactam) and six-carbon (caprolactam) lactams. This pathway uses ω-amino acids as precursors and comprises two steps. Activation of ω-amino acids catalyzed by the <I>Clostridium propionicum</I> β-alanine CoA transferase (Act) followed by spontaneous cyclization. The pathway operation was validated both <I>in vitro</I> and <I>in vivo</I>. Three metabolically engineered <I>Escherichia coli</I> strains were developed by introducing the newly constructed metabolic pathway followed by systems-level optimization, which resulted in the production of butyrolactam, valerolactam and caprolactam from renewable carbon source. In particular, fed-batch fermentation of the final engineered <I>E. coli</I> strain produced 54.14g/L of butyrolactam in a glucose minimal medium. These results demonstrate the high efficiency of the novel lactam pathway developed in this study.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Lactams are important chemicals having a wide range of industrial applications. </LI> <LI> Novel metabolic pathways for four-, five- and six-carbon lactams are constructed. </LI> <LI> β-Alanine CoA transferase is a key enzyme for the novel pathway. </LI> <LI> Production of C4, C5 and C6 lactams from renewable carbon sources are demonstrated. </LI> <LI> Fed-batch fermentation allowed production of 54g/L butyrolactam. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>