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      저자 : Tingzhen Mu (검색결과 2 건)
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        Rate-based model for predicting and evaluating H2S absorption in the haloalkaliphilic biological desulfurization process

        Zheng Chen,Gama Yang,Tingzhen Mu,Maohua Yang,Nadia A. Samak,Sumit Peh,Yunpu Jia,Xuemi Hao,Xuhao Zhao,Jianmin Xing 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.110 No.-

        The highly efficient performance of H2S absorption is the crucial indicator for haloalkaliphilic biologicaldesulfurization (HBDS) because it immediately concerns the H2S removal efficiency and pH change ofalkaline solutions. Therefore, we investigated the effect of operating parameters on the H2S absorption’sperformance under haloalkaline conditions. The gas–liquid ratio and packing height significantly improveH2S removal efficiency, from 80% to 90% and 66% to 99%, respectively. The absorption temperature had atrivial impact on the H2S removal efficiency, and the maximum value appeared at 45 C. Additionally, alloperating parameters caused pH changes that varied in the acceptable range (0.1 to 0.5) during theabsorption process. A rate-based model was successfully developed to predict the haloalkaliphilic H2Sabsorption process accurately. Moreover, this model could be implemented to effectively evaluate theHBDS system’s stability and provide reliable theoretical guidance for the industrial HBDS process toensure good process stability.

      • KCI등재

        Ribosome-binding Sequences (RBS) Engineering of Key Genes in Escherichia coli for High Production of Fatty Alcohols

        Ruonan Chen,Yilan Liu,Wei Zhong,Hao Xuemi,Tingzhen Mu,Maohua Yang,Jianmin Xing 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.4

        Fatty alcohols are widely used in the fields of food, pharmaceutical and renewable energy and so on, thus biosynthesis of fatty alcohols has attracted wide attention in recent years. However, the concentration of fatty alcohols is still at a low level. In this paper, we constructed an Escherichia coli strain with high yield of fatty alcohols through the combinatorial regulation of three key genes of fabH, fabZ, and far. Firstly, we designed new ribosomebinding sequences (RBS) of fabH and fabZ with 10-fold strengths compared to the original ones by RBS calculator, separately. Secondly, we engineered the starting strain MGL1 by replacing the original RBS with designed ones, which resulted in MGLHS (fabH-RBS1), MGLZS (fabZRBS2), and MGLHZS (fabH-RBS1/fabZ-RBS2), respectively. Thirdly, the plasmid pL1 harboring far gene was transferred into above strains to test fatty alcohols production under the induction of different isopropyl-β-D-thiogalactoside concentrations. The results showed that the concentration of fatty alcohols in strains MGLHS-far, MGLZS-far and MGLHZS-far reached 2.75 g/L, 2.54 g/L and 3.82 g/L, respectively, which increased 35%, 25% and 88% than that of the starting strain MGL1-far. The fatty alcohols reached 9.35 g/L in fed batch fermentation with MGLHZS-far, of which the main components were two saturated fatty alcohols (C14:0 and C16:0) and two unsaturated fatty alcohols (C16:1 and C18:1). The results demonstrated that combinatorial regulation of three key genes of fabH, fabZ, and far in E. coli can achieve high-yield production of fatty alcohols.

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