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Development of a Rapid Fluorescent Immunochromatographic Test to Detect Respiratory Syncytial Virus
Thuy Tien, Trinh Thi,Park, Hyun,Tuong, Hien Thi,Yu, Seung-Taek,Choi, Du-Young,Yeo, Seon-Ju MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.10
<P>Human respiratory syncytial virus (RSV) is one of the most common viruses infecting the respiratory tracts of infants. The rapid and sensitive detection of RSV is important to minimize the incidence of infection. In this study, novel monoclonal antibodies (mAbs; B11A5 and E8A11) against RSV nucleoprotein (NP) were developed and applied to develop a rapid fluorescent immunochromatographic strip test (FICT), employing europium nanoparticles as the fluorescent material. For the FICT, the limits of detection of the antigen and virus were 1.25 µg/mL and 4.23 × 10<SUP>6</SUP> TCID<SUB>50</SUB>/mL, respectively, corresponding to 4.75 × 10<SUP>6</SUP> ± 5.8 ×10<SUP>5</SUP> (mean ± SD) RNA copy numbers per reaction mixture for RSV NP. A clinical study revealed a sensitivity of 90% (18/20) and specificity of 98.18% (108/110) for RSV detection when comparing the performance to that of reverse transcription polymerase chain reaction (RT-PCR), representing a 15% improvement in sensitivity over the SD Bioline rapid kit. This newly developed FICT could be a useful tool for the rapid diagnosis of RSV infection.</P>
In Vitro Evaluation of Two Novel Antimalarial Derivatives of SKM13: SKM13-MeO and SKM13-F
Thuy-Tien Thi Trinh,김영아,홍혜리,Linh Thi Thuy Le,장하영,김순애,박현,김학성,여선주 대한기생충학ㆍ열대의학회 2022 The Korean Journal of Parasitology Vol.60 No.6
Antimalarial drugs play an important role in the control and treatment of malaria, a deadly disease caused by the protozoan parasite Plasmodium spp. The development of novel antimalarial agents effective against drug-resistant malarial parasites is urgently needed. The novel derivatives, SKM13-MeO and SKM13-F, were designed based on an SKM13 template by replacing the phenyl group with electron-donating (-OMe) or electron-withdrawing groups (-F), respectively, to reverse the electron density. A colorimetric assay was used to quantify cytotoxicity, and in vitro inhibition assays were performed on 3 different blood stages (ring, trophozoite, and schizonts) of P. falciparum 3D7 and the ring/mixed stage of D6 strain after synchronization. The in vitro cytotoxicity analysis showed that 2 new SKM13 derivatives reduced the cytotoxicity of the SKM13 template. SKM13 maintained the IC50 at the ring and trophozoite stages but not at the schizont stage. The IC50 values for both the trophozoite stage of P. falciparum 3D7 and ring/mixed stages of D6 demonstrated that 2 SKM13 derivatives had decreased antimalarial efficacy, particularly for the SKM13-F derivative. SKM13 may be comparably effective in ring and trophozoite, and electron-donating groups (-OMe) may be better maintain the antimalarial activity than electron-withdrawing groups (-F) in SKM13 modification.
홍혜리,문권모,Thuy-Tien Thi Trinh,Tae-Hui Eom,박현,김학성,여선주 대한기생충학ㆍ열대의학회 2024 The Korean Journal of Parasitology Vol.62 No.1
Antimalarial drugs are an urgently need and crucial tool in the campaign against malaria,which can threaten public health. In this study, we examined the cytotoxicity of the 9 antimalarial compounds chemically synthesized using SKM13-2HCl. Except for SKM132HCl, the 5 newly synthesized compounds had a 50% cytotoxic concentration (CC50)>100 µM, indicating that they would be less cytotoxic than SKM13-2HCl. Among the 5compounds, only SAM13-2HCl outperformed SKM13-2HCl for antimalarial activity,showing a 3- and 1.3-fold greater selective index (SI) (CC50/IC50) than SKM13-2HCl in vitro against both chloroquine-sensitive (3D7) and chloroquine -resistant (K1) Plasmodiumfalciparum strains, respectively. Thus, the presence of morpholine amide may help to effectively suppress human-infectious P. falciparum parasites. However, the antimalarialactivity of SAM13-2HCl was inferior to that of the SKM13-2HCl template compound inthe P. berghei NK65-infected mouse model, possibly because SAM13-2HCl had a lowerpolarity and less efficient pharmacokinetics than SKM13-2HCl. SAM13-2HCl was moretoxic in the rodent model. Consequently, SAM13-2HCl containing morpholine was selected from screening a combination of pharmacologically significant structures as being the most effective in vitro against human-infectious P. falciparum but was less efficient in vivo in a P. berghei-infected animal model when compared with SKM13-2HCl. Therefore, SAM13-2HCl containing morpholine could be considered a promising compound to treat chloroquine-resistant P. falciparum infections, although further optimization is crucial to maintain antimalarial activity while reducing toxicity in animals.
Yeo, Seon-Ju,Cuc, Bui Thi,Kim, Soon-Ai,Kim, Do Thi Hoang,Bao, Duong Tuan,Tien, Trinh Thi Thuy,Anh, Nguyen Thi Viet,Choi, Do-Young,Chong, Chom-Kyu,Kim, Hak Sung,Park, Hyun Elsevier Applied Science 2017 Biosensors & Bioelectronics Vol. No.
<P><B>Abstract</B></P> <P>Sensitive and rapid diagnostic systems for avian influenza (AI) virus are required to screen large numbers of samples during a disease outbreak and to prevent the spread of infection. In this study, we employed a novel fluorescent dye for the rapid and sensitive recognition of AI virus. The styrylpyridine phosphor derivative was synthesized by adding allyl bromide as a stable linker and covalently immobilizing it on latex beads with antibodies generating the unique Red dye 53-based fluorescent probe. The performance of the innovative rapid fluorescent immnunochromatographic test (FICT) employing Red dye 53 in detecting the AI virus (A/H5N3) was 4-fold and 16-fold higher than that of Europium-based FICT and the rapid diagnostic test (RDT), respectively. In clinical studies, the presence of human nasopharyngeal specimens did not alter the performance of Red dye 53-linked FICT for the detection of H7N1 virus. Furthermore, in influenza A virus-infected human nasopharyngeal specimens, the sensitivity of the Red dye 53-based assay and RDT was 88.89% (8/9) and 55.56% (5/9) relative to rRT-PCR, respectively. The photostability of Red dye 53 was higher than that of fluorescein isothiocyanate (FITC), showing a stronger fluorescent signal persisting up to 8min under UV. The Red dye 53 could therefore be a potential probe for rapid fluorescent diagnostic systems that can recognize AI virus in clinical specimens.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>