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여선주,Dong-Xu Liu,HyunPark 대한기생충학ㆍ열대의학회 2016 The Korean Journal of Parasitology Vol.54 No.1
After invasion of red blood cells, malaria matures within the cell by degrading hemoglobin avidly. For enormous protein breakdown in trophozoite stage, many efficient and ordered proteolysis networks have been postulated and exploited. In this study, a potential interaction of a 60-kDa Plasmodium falciparum (Pf)-heat shock protein (Hsp60) and Pf-calpain, a cysteine protease, was explored. Pf-infected RBC was isolated and the endogenous Pf-Hsp60 and Pf-calpain were determined by western blot analysis and similar antigenicity of GroEL and Pf-Hsp60 was determined with anti-Pf-Hsp60. Potential interaction of Pf-calpain and Pf-Hsp60 was determined by immunoprecipitation and immunofluorescence assay. Mizoribine, a well-known inhibitor of Hsp60, attenuated both Pf-calpain enzyme activity as well as P. falciparum growth. The presented data suggest that the Pf-Hsp60 may function on Pf-calpain in a part of networks during malaria growth.
여선주,박경진,이승우,유필진 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.0
The directional photo-reconfiguration of azomaterials along with the light polarization has enabled sophisticated engineering of micro/nanoarchitectures with exquisite control over large-area 2D/3D structural shapes and geometries. However, its unique feature of a path change approach to the micro/nanofabrication place severe restrictions on the range of available structural motifs and their practical applications because much of efforts have been mainly limited to a simple flat or pattern as pristine structures. To overcome this incompatible challenge,here, we have advanced the experimental conditions for the high throughput generation of azomaterial microspheres. Microfluidic approach provides a platform for the generation of uniformly sized colloidal microspheres fully made of linearized azopolymeric chain.We achieved 3D shaped colloidal microparticles and Janus microsphere with different surface textures, which could be useful for the diffractive grating-barcoded microparticles.
여선주,이기라,이대연,유필진 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.0
Structural colors based on colloidal photonic crystals (CPCs) are very useful in a variety of photonic applications because of the simplicity of tuning the photonic properties of these structures. It is extremely useful to generate flexible structural color that maintains its color regardless of viewing angle and mechanical deformations. It is challenging to simultaneously achieve these goals because anisotropy in CPC structures tend to lead to angle-dependent photonic properties and also changes in the lattice constant due to mechanical deformation leads to changes in the diffraction properties of CPCs. To overcome these challenges, we present a means of fabricating large-area free-standing films of CPC that exhibit angle- and strain-independent characteristics using a microfluidic device. The shape and crystallinity of CPC supraparticles can be tuned by osmotic annealing. Subsequently, CPC supraparticles are embedded inside an elastomeric matrix to form a flexible free-standing film.
Multidimensional assembly of s-layer proteins on mobility-controlled polyelectrolyte multilayers
여선주,유필진 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1
Bacterial surface layers (s-layers) have shown a great potential for constructing the nanostructured biomimetic surfaces. They have an intrinsic characteristic of self-reassembly, enabling them to be utilized as useful building blocks in nanofabrication process. We report that recrystallization of s-layers can be manipulated by the type of assembling substrates. When s-layers are recrystallized on weakly charged polyelectrolyte multilayers wherein unique properties of high surface mobility and interdiffusion are allowed, notably large-scale two-dimensional assembly of s-layers with few defects can be obtained. In addition, a morphological transition of s-layer crystallization from 1D hairy structures to 2D sheets is captured by controlling the temperature, which confirms the importance of the surface mobility during recrystallization process. Therefore, it is expected that various applications will be available for patterning, templated synthesis, or transporting nanochannel devices.