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RISS 인기검색어
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- 저자 : Takuji Kawanami (검색결과 2 건)
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Masato Hino,Takuji Kawanami,Jian Xu,Daisuke Morokuma,Kazuma Hirata,Mami Yamashita,Noriko Karasaki,Tuneyuki Tatsuke,Hiroaki Mon,Kazuhiro Iiyama,Noriho Kamiya,Yutaka Banno,Takahiro Kusakabe,이재민 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.2
Interleukin 2 (IL-2) is a pharmacologically vital cytokine secreted mainly by activated CD4+ T lymphocyte. Recombinant human IL-2 (rhIL-2) protein has already been globally applied as an immune-therapeutic reagent for various diseases. Therefore, there is great interest in developing an active form of rhIL-2 in very large amounts and with excellent purity for clinical use. In this study, we successfully mass-expressed and purified N- or C-terminal tandem tag-fused rhIL-2 in a baculovirus expression vector system (BEVS) using silkworm larvae as factories. We confirmed that the intrinsic instability of hIL-2 causes the loss and low recovery of N-tagged rhIL-2 and that C-tagged rhIL-2 ismore suitable for mass production. Furthermore, the activity of purified rhIL-2s was further validated by a cell proliferation assay of a human natural killer cell line, and both rhIL-2 proteins produced in the silkworm-BEVS exhibited comparable activity to that of the commercial E.coli-derived rhIL-2. Taken together, our results and strategies could contribute greatly to the mass production of active rhIL-2.
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Field Test of Contra-Rotating Small Hydroturbine
Toru Shigemitsu,Takumi Omori,Yuya Hiraishi,Takashi Tsuda,Takayuki Kawanami,Kenji Hiranuma,Takuji Hosotani,Ding Nan 한국유체기계학회 2023 International journal of fluid machinery and syste Vol.16 No.2
The contra-rotating small hydroturbine can be applied to pipelines of agricultural water and for farming river fishes. In the previous research, the advantages of the contra-rotating small hydroturbine were verified by the numerical analysis and experimental apparatus of our lab. The diameter of our test model was small as 58mm and it have to be scaled up to apply it to the actual case of a pipeline for farming river fishes. Therefore, we made the diameter of our test model larger, which is suitable for the pipeline for farming river fishes. The numerical analysis was conducted to check the power of the large-scale model for the pipeline. Furthermore, the parameter study related to the solidity, blade number and setting angle, was conducted to increase the power of the hydroturbine using the numerical analysis. After that, the suitable impeller for the field test was chosen and an experimental apparatus for the field test was established. The power generation at different flow rate conditions was measured by the experiment and the stability of the experimental apparatus; leakage flow from the mechanical seal, vibration, noise of the turbine, was checked in the field test. In this paper, our developing process of the contra-rotating small hydroturbine is shown and the prospect to apply our test turbine for the pipelines is discussed based on the numerical analysis and field test results.
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