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DNA Length Dependent Photocurrent of Diketopyrrolopyrrole Aggregates Constructed with DNA
Nakamura, Mitsunobu,Tsuto, Koji,Takada, Tadao,Yamana, Kazushige Korean Society of Photoscience 2014 Rapid communication in photoscience Vol.3 No.4
Bis(2-thienyl)-diketopyrrolopyrrole having two $Zn^{II}$-cylcens (DPPCy) was synthesized. DPP-aggregates were constructed by self-organization of DPPCy and $dT_n$-DNAs. In the presence of L-ascorbic acid as an electron sacrifice reagent, the DPP aggregates immobilized on a gold electrode exhibit good anodic photocurrent responses as well as cathodic photocurrent responses in the presence of methyl viologen. The anodic photocurrent responses depend on the DNA lengths because of the formation of uniform DPP-aggregates corresponding to the DNA lengths. The present results show that photocurrent responses of the DPP-aggregates can be controlled by DNA lengths and electron sacrifice reagents.
Takada, Tadao,Tanimizu, Yosuke,Nakamura, Mitsunobu,Yamana, Kazushige Korean Society of Photoscience 2014 Rapid communication in photoscience Vol.3 No.4
Fluorescent nucleic acids were prepared utilizing the polymerase extension (PEX) reaction to incorporate fluorescent molecules. 2'-Deoxyuridine triphosphate (dUTP) derivatives possessing pyrene molecules as fluorophores were synthesized using the aqueous-phase Sonogashira coupling between 5-Iodo-dUTP and acetylene-linked pyrene molecules. The incorporation of the pyrene (Py)-labeled deoxyuridine triphosphates (PyU) into DNA by polymerase was evaluated by polyacrylamide gel electrophoresis, demonstrating that the PyU can work as a good substrate for the PEX reaction. The fluorescent properties of the functionalized DNA prepared by the PEX reaction were characterized by steady-state fluorescence measurements. The Py-conjugated DNA showed typical emission spectra of the pyrene, and the DNA with two pyrene molecules connected to each other by a diethylene glycol linker exhibited a broadened emission attributed to the electronic interaction between the Py molecules.
Gain-based Delay Balancing Technique for Wave Pipelining
Jubee Tada,Ryusuke Egawa,Keiichiro Sano,Gensuke Goto,Tadao Nakamura 대한전자공학회 2007 ITC-CSCC :International Technical Conference on Ci Vol.2007 No.7
In this paper, we propose a delay balancing technique for wave pipelining. Wave pipelining is expected to resolve some problems in a conventional pipeline and achieve a higher frequency circuit. However, realizing wave pipelining requires high efficient delay balancing technique. To resolve this problem, we propose a delay balancing technique based on gain-based delay model for wave pipelining. Experimental results show that our approach can achieve up to 6 times throughput by increasing 23% transistors.