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Metabolism of Dimethylphthalate by Aspergillus niger
PRADEEPKUMAR, SHARANAGOUDA,KAREGOUDAR, T. B. 한국미생물 · 생명공학회 2000 Journal of microbiology and biotechnology Vol.10 No.4
Aspergillus niger is capable of metabolizing dimethylphthalate. The maximum weight of mycelium was observed after about 6-8 days of incubation. A TLC analysis revealed the accumulation of metabolites in the resting cell culture. Monomethylphthalate, phthalate, and protocatechuate were shown to be the intermediates by thin layer chromatographic and spectrophotometric analyses. The fungus metabolized dimethylphthalate through monomethylphthalate, phthalate, and protocatechuate as evidenced by the oxygen uptake and an enzymatic analysis. The terminal aromatic metabolite, protocatechuate, is metabolized via the ortho-cleavage pathway.
Properties and Functions of Melanin Pigment from Klebsiella sp. GSK
( Sajjan ),( Shrishailnath S ),( Anjaneya O ),( Guruprasad B Kulkarni ),( Anand S Nayak ),( Suresh B Mashetty ),( T B Karegoudar ) 한국미생물생명공학회(구 한국산업미생물학회) 2013 한국미생물·생명공학회지 Vol.41 No.1
Purified melanin pigment from Klebsiella sp. GSK was characterized by thermogravimetric, differential thermal, X ray diffraction and elemental analysis. This melanin pigment is structurally amorphous in nature. It is thermally stable up to 300oC and emits a strong exothermic peak at 700oC. Its carbon, hydrogen and nitrogen composition is 47.9%, 6.9% and 12.0%, respectively. It was used to scavenge metal ions and free radicals. After immobilizing the pigment and using it to adsorb copper and lead ions, the metal ion adsorption capacity was evaluated by atomic absorption spectroscopy (AAS) and the identity of melanin functional groups involved in the binding of metal ions was determined by Fourier transform infrared (FT-IR) spectroscopy. Batch adsorption studies showed that 169 mg/g of copper and 280 mg/g of lead were adsorbed onto melanin-alginate beads. The metal ion adsorption capacity of the melanin-alginate beads was relatively significant compared to alginate beads. The metal ion desorption capacity of HCl was greater (81.5% and 99% for copper and lead, respectively) than that of EDTA (80% and 71% for copper and lead, respectively). The ability of the melanin pigment to scavenge free radicals was evaluated by inhibition of the oxidation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2`-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and was shown to be about 74% and 98%, respectively, compared with standard antioxidants.
Shrishailnath S. Sajjan,M. Santoshkumar,S. Sanjeevkumar,T. B. Karegoudar 한국약제학회 2013 Journal of Pharmaceutical Investigation Vol.43 No.4
Amlodipine, atorvastatin and telmisartan are the most commonly used pharmaceutical drugs against cardiovascular disease, management of hypertension and in the treatment of angina. They have side-effects like edema,allergic reactions, breathing problems, etc. The melanin pigment from Klebsiella sp. GSK has the ability to adsorb pharmaceutical drugs, metals ions and to scavenge free radicals. We have studied here the binding of these drugs to melanin pigment at different concentrations and temperatures. It shows that these drugs in higher concentrations form stable complexes with melanin pigment. The amount of drug bound to melanin increases with increase in initial drug concentration. The drug-binding properties of melanin pigment were investigated kinetically. Telmisartan and amlodipine bind strongly at 40 C (59 and 25.8 lg/mg,respectively) than atorvastatin (18 lg/mg). Batch adsorption,uptake kinetics, Langmuir and Freundlich equations and temperature adsorption studies were also conducted with melanin pigment.
Stable Degradation of Benzoate by Klebsiella oxytoca C302 Immobilized in Alginate and Polyurethane
김치경,Jun-Ho Kim,Won-Hwa Jeong,T. B. Karegoudar 한국생물공학회 2002 Biotechnology and Bioprocess Engineering Vol.7 No.6
Benzoate produced from the degradative pathways of various aromatic chemicals isgeneraly recognized as a polutant compound. However, various bacterial strains isolated as benzoate degraders have exhibited certain limits to their functions, including a los of viability and degradability when cultivated in a broth medium for a longer time. Acordingly, immobilization techniques have been utilized to overcome such problems, and the curent study examined the use of alginate and polyurethane for immobilizing C302 to extend its viability and degradability of benzoate. The organism was well encapsulated by both matrices and the immobilized cels showed a high stability as regards their viability and degradability of 2 mM benzoate in a MM2 broth medium during cultivation for longer than 60 h in a semicontinuous batch system.
Purification and Physiochemical Characterization of Melanin Pigment from Klebsiella sp. GSK
( Sajjan,Shrishailnath ),( Guruprasad Kulkarni ),( Veeranagouda Yaligara ),( Kyoung Lee ),( T. B. Karegoudar ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.11
A bacterium capable of producing melanin pigment in the presence of L-tyrosine was isolated from a crop field soil sample and identified as Klebsiella sp. GSK based on morphological, biochemical, and 16S rDNA sequencing. The polymerization of this pigment occurs outside the cell wall, which has a granular structure as melanin ghosts. Chemical characterization of the pigment particles showed then to be acid resistant, alkali soluble, and insoluble in most of the organic solvents and water. The pigment got bleached when subjected to the action of oxidants as well as reductants. This pigment was precipitated with FeCl3, ammoniacal silver nitrate, and potassium ferricynide. The pigment showed high absorbance in the UV region and decreased absorbance when shifted towards the visible region. The melanin pigment was further charecterized by FT-IR and EPR spectroscopies. A key enzyme, 4- hydroxyphenylacetic acid hydroxylase, that catalyzes the formation of melanin pigment by hydroxylation of Ltyrosine was detected in this bacterium. Inhibition studies with specific inhibitors, kojic acid and KCN, proved that melanin is synthesized by the DOPA-melanin pathway.