http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Sujata Acharjee (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
Sujata Acharjee,Kaushik Maiti,Jae Mok Soh,Wook-Bin Im,Jae Young Seong,Hyuk Bang Kwon 한국분자세포생물학회 2002 Molecules and cells Vol.14 No.1
We previously demonstrated the presence of three dis-tinct types of the gonadotropin-releasing hormone re-ceptor (GnRHR) in a bullfrog (denoted bfGnRHR-1, bfGnRHR-2, and bfGnRHR-3). The bfGnRHRs exhib-ited differential tissue distribution and ligand selectiv-ity. In the present study, we demonstrated the desensi-tization and internalization kinetics of these receptors in both transiently-transfected HEK293 cells and ret-rovirus-mediated stable cells. The time-course accu-mulation of the inositol phosphate in response to GnRH revealed that bfGnRHR-1 and -2 were rapidly desensitized, whereas bfGnRHR-3 was slowly desensi-tized. A comparison of the internalization kinetics re-vealed the most rapid rate and highest extent of inter-nalization of bfGnRHR-2 among the three receptors. Interestingly, the mechanisms that underlie the recep-tor internalization appear to differ from each other. Internalization of bfGnRHR-1 was dependent on both dynamin and b-arrestin, whereas those of bfGnRHR-2 and -3 were dependent on dynamin, but not on b-arrestin. These results, therefore, suggest that differen-tial regulatory mechanisms for desensitization and internalization of the GnRHR are involved in diverse cellular and physiological responses to GnRH stimula-tion.
-
Jae Young Seong,Kaushik Maiti,Jian Hua Li,Ai Fen Wang,Sujata Acharjee,Wang Phil Kim,임욱빈,권혁방 한국분자세포생물학회 2003 Molecules and cells Vol.16 No.2
ecently, we identified three types of non-mammalian gonadotropin-releasing hormone receptors (GnRHR) in the bullfrog (designated bfGnRHR-1-3), and a mammalian type-II GnRHR in green monkey cell lines (denoted gmGnRHR-2). All these receptors responded better to GnRH-II than GnRH-I, while mammalian type-I GnRHR showed greater sensitivity to GnRH-I than GnRH-II. In the present study, we designed new GnRH-II analogs and examined whether they acti- vated or inhibited non-mammalian and mammalian type-II GnRHRs. [D-Ala 6 ]GnRH-II, with D-Ala substi- tuted for Gly 6 in GnRH-II, increased inositol phos- phate (IP) production in cells stably expressing non- mammalian GnRHRs more effectively than native GnRH-II. However, it exhibited lower activity for mammalian type-I GnRHR than GnRH-I itself. Trptorelix-1, a GnRH-II antagonist, inhibited GnRH- induced IP production in cells expressing non- mammalian GnRHRs more effectively than Cetrorelix, a GnRH-I antagonist. Trptorelix-1, however, had lower potency for mammalian type-I GnRHR than Cetrorelix. Ligand-receptor binding assays revealed that [D-Ala 6 ]GnRH-II and Trptorelix-1 have higher affinities for non-mammalian GnRHRs but lower af- finities for mammalian type-I GnRHR than GnRH-II and Cetrorelix, respectively. Moreover, [D-Ala 6 ]GnRH- II and Trptorelix-1 had a higher affinity for gmGnRHR-2 than GnRH-II and Cetrorelix, respec- tively. These results indicate that [D-Ala 6 ]GnRH-II and Trptorelix-1 are highly effective agonist and antagonist, respectively, for non-mammalian and type- II mammalian GnRHRs
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
