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- 저자 : Solem, C. (검색결과 3 건)
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Carcass and Meat Quality Traits in an Embden×Toulouse Goose Cross Raised in Organic Dehesa
Sole, M.,Pena, F.,Domenech, V.,Clemente, I.,Polvillo, O.,Valera, M.,Verona, J.C.,Rubi, M.,Molina, A. Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.6
This study assessed the influence of genetic type (Embden-Anser anser, EE; Toulouse-Anser anser, TT and F1 cross, ET) for meat characteristics (carcass, meat quality and fatty acid (FA) profiles), of domestic geese "Anser anser domesticus" raised in dehesa as an alternative, organic feeding system. Carcass and breast muscle weight (p<0.01) were greater for the ET group at the same live weight. None of the groups showed differences in the production of fatty liver with this type of feeding. Higher values were found for maximum Warner-Bratzler shear force (between 7.62 and $8.87kg/cm^2$), which implies the improvement of this parameter. High levels of oleic FAs were obtained, especially for the TT group. The polyunsaturated/saturated FA ratio was highest for the ET group (p<0.001), reflecting the optimum nutritional values as a component of a healthy consumer diet.
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Liu, Jianming,Wang, Zhihao,Kandasamy, Vijayalakshmi,Lee, Sang Yup,Solem, Christian,Jensen, Peter Ruhdal Elsevier 2017 Metabolic engineering Vol.44 No.-
<P><B>Abstract</B></P> <P>When modifying the metabolism of living organisms with the aim of achieving biosynthesis of useful compounds, it is essential to ensure that it is possible to achieve overall redox balance. We propose a generalized strategy for this, based on fine-tuning of respiration. The strategy was applied on metabolically engineered <I>Lactococcus lactis</I> strains to optimize the production of acetoin and (<I>R</I>,<I>R</I>)-2,3-butanediol (R-BDO). In the absence of an external electron acceptor, a surplus of two NADH per acetoin molecule is produced. We found that a fully activated respiration was able to efficiently regenerate NAD<SUP>+</SUP>, and a high titer of 371mM (32g/L) of acetoin was obtained with a yield of 82% of the theoretical maximum. Subsequently, we extended the metabolic pathway from acetoin to R-BDO by introducing the butanediol dehydrogenase gene from <I>Bacillus subtilis</I>. Since one mole of NADH is consumed when acetoin is converted into R-BDO per mole, only the excess of NADH needs to be oxidized via respiration. Either by fine-tuning the respiration capacity or by using a dual-phase fermentation approach involving a switch from fully respiratory to non-respiratory conditions, we obtained 361mM (32g/L) R-BDO with a yield of 81% or 365mM (33g/L) with a yield of 82%, respectively. These results demonstrate the great potential in using finely-tuned respiration machineries for bio-production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Fine-tuning of the reducing power availability via respiration. </LI> <LI> Respiration capacity modulated through hemin concentration. </LI> <LI> High titer and yield production of acetoin by harnessing respiration. </LI> <LI> Production of (R,R)-2,3-butanediol by using a fine-tuned respiration. </LI> <LI> A dual phase fermentation approach developed for (R,R) 2,3-butanediol biosynthesis. </LI> </UL> </P>
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Liu, J.,Chan, S.H.J.,Brock-Nannestad, T.,Chen, J.,Lee, S.Y.,Solem, C.,Jensen, P.R. Academic Press 2016 Metabolic engineering Vol.36 No.-
Biocompatible chemistry is gaining increasing attention because of its potential within biotechnology for expanding the repertoire of biological transformations carried out by enzymes. Here we demonstrate how biocompatible chemistry can be used for synthesizing valuable compounds as well as for linking metabolic pathways to achieve redox balance and rescued growth. By comprehensive rerouting of metabolism, activation of respiration, and finally metal ion catalysis, we successfully managed to convert the homolactic bacterium Lactococcus lactis into a homo-diacetyl producer with high titer (95mM or 8.2g/L) and high yield (87% of the theoretical maximum). Subsequently, the pathway was extended to (S,S)-2,3-butanediol (S-BDO) through efficiently linking two metabolic pathways via chemical catalysis. This resulted in efficient homo-S-BDO production with a titer of 74mM (6.7g/L) S-BDO and a yield of 82%. The diacetyl and S-BDO production rates and yields obtained are the highest ever reported, demonstrating the promising combination of metabolic engineering and biocompatible chemistry as well as the great potential of L. lactis as a new production platform.
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