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- 저자 : Sixing Yang (검색결과 2 건)
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Yunhe Xiong,Wen-Biao Liao,Sixing Yang,Lingchao Meng,Chao Song 한국조직공학과 재생의학회 2015 조직공학과 재생의학 Vol.12 No.3
Ileal conduits are commonly used in bladder cancer treatment and in pediatric patients who require urinary diversion surgeries. We constructed a tissue-engineered conduit using bone marrow mesenchymal stem cells (BMSCs) and a bladder acellular matrix (BAM) and transplanted it into rabbits for urinary diversion to evaluate the feasibility of its clinical application. Rabbit BMSCs were isolated, expanded in vitro, and were then treated with a conditional medium for 3 weeks, allowing the BMSCs to transform into urothelium-like cells. These cells were seeded onto BAM and cultured for another week. The cell-matrix grafts were then sewn into a conduit approximately 4 cm in length and 1 cm in diameter and were implanted as conduits for urinary diversion in 12 male rabbits. Rabbits were sacrificed at 1, 2, and 4 week after operation. Histologic examinations were performed using hematoxylin and eosin staining and tissue structures were evaluated by using immunohistochemistry. BMSCs can transform into urothelium-like cells that express the urothelium-specific proteins uroplakin 1A (UPK1A), cytokeratin 7 (CK7), and cytokeratin 13 (CK13). These cells are able to generate epithelial coverage in the conduit lumen based on BAM. Immunohistochemistry showed positive staining with AE1/AE3, UPK1A, and tight junction protein 1 (ZO-1), indicating the presence of mature and functional epithelial cells on the lumen of the conduit. BMSCs may represent a new source cell for urinary tissue engineering, and it is feasible to construct a conduit with transformed BMSCs and BAM for urinary diversion in rabbits.
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국가 온실가스 인벤토리 축산부문 작성을 위한 온실가스 배출계수 개발 현황에 관한 연구
이진의,이현주,박규현,최병양,라창식,Lee, Jin Eui,Lee, Hyun Ju,Park, Kyu Hyun,Choi, Byong Yang,Ra, Chang Six 한국축산환경학회 2011 축산시설환경학회지 Vol.17 No.suppl.
In this study, researches on the development of country specific greenhouse gas measurement and sampling methods from the livestock sector were reviewed. Research on greenhouse gas emission factors was started in early 2000 but was not actively involved in the development of livestock sector based emission factor: since 2009, works are underway for the development of livestock sector based emission factor. Most of the research on greenhouse gas emission in the field of animal studies were done by National Institute of Animal Science, because of the uniqueness of the research laboratories. Methods of emission measurement are still not internationally certified and therefore, measurement and sampling methods for Korea livestock sector are being studied, consulting the worldwide research trends. Flux chamber method are commonly using in Korea for green house gas emission factors measurement. In recent years, continuous measurement of the microclimate was introduced to measure greenhouse gases from livestock manure storage facilities and the micrometeorology method should be adapted as recommended by Intergovernmental Penal on Climate Change (IPCC) Guideline.
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