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Sivaprasad, V.,Nataraju, B.,Renu, S.,Datta, R.K. Korean Society of Sericultural Science 2003 International Journal of Industrial Entomology Vol.6 No.1
Infectious flacherie of silkworm Bombyx mori is caused by B. mori infectious flacherie virus (BmIFV) and causes severe crop loss to sericulturists. In the present study, a colloidal textile dye-based dipstick immunoassay is developed for the detection of infectious flacherie in silkworms. Colloidal textile dye (blue D2R) with Aλ$_{max}$ at 620 nm was sensitised with 500 $\mu\textrm{g}$/ml of purified anti-BmIFV IgG. The dye-antibody reagent detects purified antigen up to 10 ng/ml and BmIFV infection in diseased larval extracts $(up to a dilution of {10^-5})$ and faecal matter extracts $(up to a dilution of {10^-2})$ by forming clear blue dot within 30 min. It was observed to be stable for three months period at $4^{\circ}C$. The efficacy of textile dye-based dipstick immunoassay was on pay with HRP-based dipstick immunoassay and fluorescent antibody test, and better than latex agglutination and ouchterlony tests in the detection of BmIFV The dye-based dipstick immunoassay method provides a simple, sensitive and less expensive test for the detection of BmIFV infection in silkworms.s.
$F(ab)_2$-ELISA for the Detection of Nuclear Polyhedrosis Virus of Silk-worm, Bombyx mori L.
Sivaprasad, V.,Nataraju, B.,Baig, M.,Samson, M.V.,Datta, R.K. Korean Society of Sericultural Science 2003 International Journal of Industrial Entomology Vol.6 No.2
$F(ab`)_2$-ELISA and direct antigen coating-ELISA (DAC-ELISA) were evaluated in the detection of purified Bombyx mori nuclear polyhedrosis virus (BmNPV) and nuclear polyhedrosis virus infection in silkworm larvae inoculated with BmNPV polyhedra. Although nanogram levels of BmNPV was detected in both DAC- and $F(ab`)_2$-ELISA, similar concentrations of antigen was detected in case of F(ab’)$_2$-ELISA even at higher dilution of antibody (up to 1 : 20 K). One hundred percent nuclear polyhedrosis infection was detected 6 hrs after inoculation in BmNPV infected silkworm larvae by $F(ab`)_2$-ELISA. On the other hand, detection of 100% infection was observed only three days after inoculation in DAC-ELISA. In this study, it was observed $F(ab`)_2$-ELISA was more sensitive than DAC-ELISA in the detection of purified BmNPV as well as nuclear polyhedrosis infection in silkworm larvae.
KUMAR, S. SIVAPRASAD,TANEJA, H. C.,RAVICHANDRAN, V. 대한수학회 2006 Kyungpook mathematical journal Vol.46 No.1
In this paper, the authors introduce new classes of p-valent functions defined by Dziok-Srivastava linear operator and the multiplier transformation and study their properties by using certain first order differential subordination and superordination. Also certain inclusion relations are established and an integral transform is discussed.
Kumar, S. Sivaprasad,Taneja, H.C.,Ravichandran, V. Department of Mathematics 2006 Kyungpook mathematical journal Vol.46 No.1
In this paper, the authors introduce new classes of p-valent functions defined by Dziok-Srivastava linear operator and the multiplier transformation and study their properties by using certain first order differential subordination and superordination. Also certain inclusion relations are established and an integral transform is discussed.
On a Class of Meromorphic Functions Defined by Certain Linear Operators
Kumar, Shanmugam Sivaprasad,Taneja, Harish Chander Department of Mathematics 2009 Kyungpook mathematical journal Vol.49 No.4
In the present investigation, we introduce new classes of p-valent meromorphic functions defined by Liu-Srivastava linear operator and the multiplier transform and study their properties by using certain first order differential subordination and superordination.
Effect of Top Sheet Materials on Joint Performance of Self-Piercing Riveting
Karim Md Abdul,Murugan Sivaprasad,배기만,백종진,지창욱,노우람,이한주,Jang Will,김덕봉,박영도 대한용접접합학회 2022 대한용접·접합학회지 Vol.40 No.6
Three types of self-piercing riveting (SPR) joints, i.e., steel/aluminum, carbon fiber reinforced polymer (CFRP)/ aluminum, and aluminum/aluminum, were constructed using three different top sheet materials with the same alumi- num alloy as the bottom sheet. The effects of the top sheet material on the joint quality and mechanical behavior were evaluated. The top sheet materials’ characteristics dominate the rivet piercing process and the consequent inter- lock distance. The high-strength steel top sheet requires a comparatively higher rivet setting force and induces early flaring of the rivet tail, resulting in a larger interlock distance. Though the CFRP needs the highest rivet setting force to penetrate the rivet through the CFRP fibers, the CFRP/aluminum joint exhibits the smallest interlock distance be- cause of the SPR process-induced damages to the CFRP and subsequently less flaring of the rivet tail. In strength tests, the damaged CFRP sheet resulted in rivet head pullout of the CFRP/aluminum joints, which exhibited the low- est lap-shear and cross-tension strengths. In contrast, the steel/aluminum joints demonstrated the highest strengths because of their comparatively larger interlock distances. In addition to the experimental analysis, simulations re- vealed the rivet penetration and flaring mechanisms with various top sheet materials, and their respective joint qual- ity and strengths.
Fluorescent Pseudomonas Induced Systemic Resistance to Powdery Mildew in Mulberry (Morus spp.)
Pratheesh Kumar, Padinjare Mannath,Sivaprasad, Vankadara Korean Society of Sericultural Science 2017 International Journal of Industrial Entomology Vol.35 No.2
Native fluorescent pseudomonas bacteria were isolated from rhizosphere soil of mulberry and were evaluated against powdery mildew. In vitro conidial germination study showed significant (P<0.05) variation in conidial germination by bacterial strains Pf1 and Pf3. Mildew incidence was significantly varied due to treatment with various pseudomonas strains in vivo. Significantly (P<0.05) less mildew incidence was in plants treated with the bacterial strain Pf1 (9.11%) followed by Pf3 (13.48%) controlling 69.40% and 54.75% respectively compared with untreated control. Similarly, mildew severity was least (8.51%) in plants treated with strain Pf1 followed by Pf5 (9.23%) and Pf3 (9.72%) controlling the severity by 84.51%, 77.01% and 71.96% respectively compared with control. The bacterial strains significantly influenced biochemical constituents such as chlorophyll, protein and soluble sugar content of the mulberry leaf. Similarly, bacterial strains significantly increased the activity of the peroxidase (PO) and Polyphenol oxydase (PPO) activity from $7^{th}$ day up to the $28^{th}$ day after treatment. The strain Pf1, Pf3 and Pf5 exhibited a marked enhancement in the peroxidase at different periods of infection. Significant (P<0.01) negative correlation was found between powdery mildew severity with phenol content ($R^2=0.67$) as well as peroxidase ($R^2=0.92$) and polyphenol oxidase ($R^2=0.72$) activity thus confirms induction of systemic resistance in mulberry by pseudomonas bacteria. The study shows scope for exploration of rhizosphere fluorescent pseudomonas bacteria for induction of systemic resistance in mulberry to contain powdery mildew disease effectively.