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The Use of qNMR for Quality Control of Coumarin-based Pharmaceuticals and Plant Medicines
Luana C. Crocoli,Vinícius B. Molon,Sidnei Moura 한국생약학회 2021 Natural Product Sciences Vol.27 No.2
The Coumarin (1,2-benzopyrone) is the main secondary metabolite of Mikania laevigata Sch. Beep ex Baker and Mikania glomerata Spreng., which are popularly known as guaco. These plants have been used mainly in traditional medicine in the treatment of respiratory diseases because their bronchodilator effect. However, there are around 200 species of Mikania, which are quite similar in appearance. From these, only M. leavigata and M. glomerata have high concentrations of coumarins. In this line, the falsification of products Mikania based has been frequent. In this sense, this work demonstrated the application of the easy, fast, e not destructive method based in Nuclear Magnetic Resonance in quantitative mode (qNMR) for the determination of coumarin in both commercial and homemade guaco products. Thus, in the first step the compounds were extract from guaco leaves and syrups using chloroform (CHCl3), with or without ultrasound. About the method, was linear with a R2= 0.9947 for 1,2-benzopyrone, with detection and quantification limits with were 0.11 and 0.36 mg mL-1 respectively. In the same line, the method was safe with RSD <0.3% and with recovery ranging from 93-101%. To confirm the applicability of the method, in the last step was applied to 10 real samples (6 from leaves and 4 from syrups). The content of the coumarin in the leaf extract ranged from 0.62 to 1.30 mg mL-1. For syrups I, II and IV, the content of coumarin was in accordance with the manufacturers. However, for de Syrup III, the concentration was 155% higher. In summary, the qNMR is a rapid method with minimal sample preparation that can be used to quantify coumarin in home-made plant extracts as well as in commercial samples as syrup for instance. This method is applicable for quality control of different plants-based products.
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Vania Pezzini,Fabiana Agostini,Franco Smiderle,Luciana Touguinha,Mirian Salvador,Sidnei Moura 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.3
By-products of the grape juice industry containvaluable compounds. The current work produced bioactive-enriched extracts from by-products of the grape juice,through three different extraction methods. Yields andchemical compositions varied, according to the extractionmethod (ultrasound, microwave, liquid–liquid). High-efficiencyliquid chromatography with UV–Vis and high-resolutionmass spectrometry characterised were used forchemical characterization, with glycosylated flavonoidsevident. The crude extract was fractionated by open column,which has possibility carried-out fraction rich inresveratrol. The inhibition of DPPH radicals ranged from14.2 to 74.2%, and the total phenolic content ranged from0.1 to 107.0 mg gallic acid equivalents/100 g. Microwaveassistedextraction of grape juice by-products using polarsolvents, such as ethanol and water, provided the best yieldand chemical composition, obtaining extracts rich in flavonoids. In this way, this work has demonstrated theindustrial grape by-products importances, which are a richsource of antioxidants if properly extracted.
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