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- 저자 : Shuyi Guo (검색결과 2 건)
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Shuyi Yang,Yang-bin Ou,Yong Guo,Xian-ming Wu 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.18 No.7
An impact system is the core part of the hydraulic rock drill. The dynamic simulation model of the hydraulic impact system is established based on the system simulation platform AMEsim. We used the AMEsim simulation model to verify the displacement, velocity curves of the piston, and valve by using stress wave testing data. The following key features of the impact system are determined: the characteristic curves of the pressure variations in the piston chambers, the influences of oil flow, the charging pressures of the high-pressure accumulator, the pre-setting pressure of the relief valve on impact performance, and impact efficiency. On the basis of orthogonal experiments, a response surface method is used to build a mathematical model of the impact system with efficient and various working parameters. The impact system with an optimal efficiency is designed, and the working parameters are optimized. Our proposed system efficiency increases by 17.7% after the working parameters are optimized. This study provides a theoretical basis for the design and optimization of the impact system parameters of the hydraulic rock drill with a sleeve valve.
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Mingchang Li,Shuyi Guo,Xiaomin Li,Quan Wang,Lin Zhu,Chunyan Yin,Wei Wang 한국생물공학회 2017 Biotechnology and Bioprocess Engineering Vol.22 No.6
Thermophilic or hyperthermophilic SODs (superoxide dismutase) usually offer substantial biotechnological advantages over mesophilic SODs. Previously a 244-amino acid N-terminal domain (NTD) from a heatresistant SOD of Geobacillus thermodenitrificans NG80-2 was discovered and demonstrated to be able to confer thermostability to homologous mesophilic SODs, which revealed a new type of heat resistance mechanism. To further improve the heat resistance and stress tolerance of thermophilic cambialistic superoxide dismutase (Fe/Mn- SODAp) from Aeropyrum pernix K1 through metal incorporation and fusion with the newly found peptide NTD for broadening its industrial application, the wildtype SODAp and NTD-fused ntdSODAp were expressed in E. coli BL21 and incorporated with metal cofactors by two ways. Recombinant fusion SOD obtained by in vitro reconstitution (Mn-rec ntdSODAp) exhibited improved optimum temperature at 70oC and dramatically enhanced thermostability especially at 110oC with enhanced pH stability from 4 to 10 and higher tolerance for denaturants and organic media than Mn-rec SODAp. To the best of our knowledge, Mn-rec ntdSODAp could be the most heat resistant SOD. In addition, metal incorporation of SODAp and ntdSODAp via in vivo modification have been developed and proved to be more practical for industrial use. These results indicate that fusion with NTD along with metal incorporation can generate superimposed effect and be applied to enhance the stability of cambialistic thermophilic SODs, thus providing a universal and convenient bioengineering method for generating extremely stable SODs.
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