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Identification and Functional Characterization of R3 MYB Transcription Factor Genes in Soybean
Shucai Wang,Jinsong Pang,Hongwei Xun,Zhibing Zhang,Yunxiao Zhou,Xueyan Qian,Yingshan Dong,Xianzhong Feng,Bao Liu 한국식물학회 2018 Journal of Plant Biology Vol.61 No.2
In Arabidopsis, trichome formation is regulatedby a MYB-bHLH-WD40 (MBW) transcriptional activatorcomplex, which can activate the expression of GLABRA2(GL2) and R3 MYB genes. GL2 is required for trichomeformation, whereas R3 MYBs inhibit trichome formation byblocking the formation of the MBW complex, thus inhibitingthe expression of GL2. By using the amino acid sequence of theArabidopsis R3 MYB transcription factor TRICHOMELESS1(TCL1) to BLAST the soybean (Glycine max) protein database,we found that there are a total of six R3 MYB genes insoybean, namely Glycine max TRICHOMELESS1 through 6(GmTCL1-GmTCL6). By generating transgenic plants, wefound that trichome formation in soybean plants overexpressingeach of the GmTCLs remained largely unchanged, and theexpression of putative GL1 and GL2 genes in the transgenicplants was unaffected. However, all the GmTCLs interactedwith GLABRA3 (GL3) in transfected Arabidopsis protoplasts,expression each of the GmTCLs in Arabidopsis inhibitedtrichome formation, and the expression levels of GL1 andGL2 were greatly reduced in the Arabidopsis transgenicplants. Moreover, phenotypic complementary analysis showedthat GmTCL1 is functionally equivalent to TCL1. Takentogether, these results suggest that GmTCLs are functionalR3 MYBs, however, they do not regulate trichome formationin soybean.
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Study of the Electric Field Screening Effect for Field Emitter Arrays
Nannan Li,Fei Yan,Shucai Pang,Lei Chen,Dazhi Jin,Wei Xiang,De Zhang,Jingyi Dai,Baoqing Zeng 한국물리학회 2015 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.66 No.8
Understanding the influence of the interaction of emitters on the field emission performance of a field emitter array is of great importance for the design of a multi-emitter device. In this work, a screening factor S is defined to quantify the effect of electric field screening due to the interaction of emitters in an array. A three-dimensional finite integration technique is used to study the electric field screening effect for Spindt-type field emitter arrays which have potential applications in flatscreen displays and high-power vacuum electronics. The dependences of the electric field screening on the tip-to-tip spacing, half-angle of the emitter, height of the emitter and radius of the tip’s curvature are analyzed. The effects of the variations in the size of the emitter’s geometry on the most efficient arrangement to achieve maximum emission efficiency in an array are discussed.
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