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INACTIVATION OF PHAGE QβBY 254 nm UV LIGHT AND TITANIUM DIOXIDE PHOTOCATALYST
Nakamura,Miyako,Lee,Seockheon,Ohgaki,Shinichiro 이화여자대학교 환경문제연구소 1998 이화환경연구 Vol.2 No.-
The disinfection efficacy of UV light irradiation at wavelength of 254 nm over a titanium dioxide(TiO₂) suspension was compared to that of UV alone. Bacteriophage QB was used as a model virus for the study. QB in sterilized pure water and TiO₂ suspension was irradiated by a 0.4m/㎠ intensity of 254nm UV light. The UV light over TiO₂was more effective than 254nm UV alone in inactivating QB. 3.5-log()QB inactivation was achieved by UV irradiation over TiO₂ suspension (10³mg/L) after 2 minutes of irradiation, While UV alone inactivated 2-log(). Using a MPN-PCR method, a ca 1-log()-unit decrease in QB RNA concentration was detected after 3 minutes of photocatalytic irradiation. The decrease was explained by damage to nucleic acid of phage QB due to radical oxidation, which is generated by photocatalysis.
Myoung Goo Hwang,Jung Woo Oh,Hiroyuki Katayama,Shinichiro Ohgaki,Jin Kyu Cho 대한환경공학회 2012 Environmental Engineering Research Vol.17 No.1
In this study, multiparametric flow cytometry (FCM) was installed to enumerate the diagnosis of Pseudomonas aeruginosa ATCC 10145 and Escherichia coli K12 (IFO 3301). The nucleic acids (DNA/RNA) were double stained by a LIVE/DEAD bacLight viability kit, involving green SYTO 9 and red propidium iodide (PI), based on the permeability of two chemicals according to the integrity of plasma membrane. As the results showed, the gate for dead bacteria was defined as the range of 0.2 × 100 to 6.0 × 101 photo multiplier tube (PMT) 2 fluorescence (X-axis) and 2.0 × 100 to 2.0 × 102 PMT 4 fluorescence (Y-axis), and the gate for live bacteria was defined as the range of 6.0 × 100 to 6.0 × 102 PMT 2 fluorescence (X-axis) and 2.0 × 100 to 4.0 × 102 PMT 4 fluorescence (Y-axis). In the comparison of the number of the tested bacteria detected by FCM (viability assessment) and plate culture (cultivability assessment), the number of bacteria detected by FCM well represented the number of bacteria that was detected by the colony forming unit (CFU) counting method when bacteria were exposed to isopropyl alcohol and silver/copper cations. Consequently, it is concluded that the application of FCM to monitor the functional effect of disinfectants on the physiological status of target bacteria can offer more rapid and reliable data than the plate culture colony counting method.
Hwang, Myoung-Goo,Oh, Jung-Woo,Katayama, Hiroyuki,Ohgaki, Shinichiro,Cho, Jin-Kyu Korean Society of Environmental Engineers 2012 Environmental Engineering Research Vol.17 No.1
In this study, multiparametric flow cytometry (FCM) was installed to enumerate the diagnosis of Pseudomonas aeruginosa ATCC 10145 and Escherichia coli K12 (IFO 3301). The nucleic acids (DNA/RNA) were double stained by a LIVE/DEAD bacLight viability kit, involving green SYTO 9 and red propidium iodide (PI), based on the permeability of two chemicals according to the integrity of plasma membrane. As the results showed, the gate for dead bacteria was defined as the range of $0.2{\times}10^0$ to $6.0{\times}10^1$ photo multiplier tube (PMT) 2 fluorescence (X-axis) and $2.0{\times}10^0$ to $2.0{\times}10^2$ PMT 4 fluorescence (Y-axis), and the gate for live bacteria was defined as the range of $6.0{\times}10^0$ to $6.0{\times}10^2$ PMT 2 fluorescence (X-axis) and $2.0{\times}10^0$ to $4.0{\times}10^2$ PMT 4 fluorescence (Y-axis). In the comparison of the number of the tested bacteria detected by FCM (viability assessment) and plate culture (cultivability assessment), the number of bacteria detected by FCM well represented the number of bacteria that was detected by the colony forming unit (CFU) counting method when bacteria were exposed to isopropyl alcohol and silver/copper cations. Consequently, it is concluded that the application of FCM to monitor the functional effect of disinfectants on the physiological status of target bacteria can offer more rapid and reliable data than the plate culture colony counting method.