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RISS 인기검색어
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- 저자 : Shikha Kumari (검색결과 3 건)
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Shikha Kumari,Abha Mishra,Divakar Singh,Chenzhong Li,Pradeep Srivastava 한국생물공학회 2023 Biotechnology and Bioprocess Engineering Vol.28 No.1
This research study deals with the development of copper nanoparticles (CN) and nano-hydroxyapatite (nHAP) infused chitosan (C) and gelatin (G) based nanocomposite scaffolds for bone tissue engineering applications. Human-origin osteoblast cells (MG-63) were seeded over the scaffolds to investigate the novel biomimetic extracellular matrix system. The scanning electron microscopy (SEM) showed an average pore size between 100-146 μm for all the C-G-nHAP-CN based scaffolds. The in-vitro degradation study showed 74-83% degradation for CN-based scaffolds. For 0.03% CN based scaffold degradation rate (84%) was very close to the control scaffold. Swelling ratio was highest for the chitosan scaffold and it was in the range between 5.25-5.93 mg/mL for other scaffolds. Compressive moduli were highest for 0.03% CN scaffold (3.32 MPa) which was relatively very high in comparison to C-G-nHAP scaffold with 2.4 MPa strength in a wet state. Stress-strain graphs also show the maximum displacement by 0.03% CN scaffold. The functional and structural analysis for the scaffolds showed the presence of nHAP in the scaffold and CN peaks within the composite structure. Differential scanning colorimetry testing showed reduced crystallinity in CN-based scaffolds with a melting temperature of 320ºC. Their 2D cell behaviour in the Electrical Cell Impedance System (ECIS) study showed maximum cell spreading and growth in 0.02% CN-based scaffold. The cell-seeded composite was tested for 3-(4,5-dimethylthiazolyl-2)-2,5- diphenyltetrazolium bromide (MTT), 4,6-diamidino-2- phenylindole (DAPI), and acridine orange and propidium iodide (AOPI) assay for testing its cytocompatibility for MG-63 cell line. Cell proliferation and cell spreading was observed by SEM in all the CN-based scaffolds. Alkaline phosphatase (ALP) activity was highest in 0.03% CN scaffold with 2.0 optical density (OD) value. Alizarin Red Stain (ARS) staining was performed to support this study. It can be statistically depicted that nHAP and 0.03% CNbased scaffold could be potential biomaterial for minor to severe bone-related tissue regeneration applications.
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Chandra Bhushan Mishra,Shikha Kumari,Manisha Tiwari 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.5
A series of 1-phenyl-3/4-[4-(aryl/heteroaryl/alkyl-piperazine1-yl)-phenyl-urea derivatives (29–42) weredesigned, synthesized and evaluated for their anticonvulsantactivity by using maximal electroshock (MES), subcutaneouspentylenetetrazole (scPTZ) seizure tests. Theacute neurotoxicity was checked by rotarod assay. Most ofthe test compounds were found effective in both seizuretests. Compound 30 (1-{4-[4-(4-chloro-phenyl)-piperazin-1-yl]-phenyl}-3-phenyl-urea) exhibited marked anticonvulsantactivity in MES as well as scPTZ tests. The phaseII anticonvulsant quantification study of compound 30indicates the ED50 value of 28.5 mg/kg against MESinduced seizures. In addition, this compound also showedconsiderable protection against pilocarpine induced statusepilepticus in rats. Seizures induced by 3-mercaptopropionicacid model and thiosemicarbazide were significantlyattenuated by compound 30, which suggested its broadspectrum of anticonvulsant activity. Interestingly, compound30 displayed better antidepressant activity thanstandard drug fluoxetine. Moreover, compound 30appeared as a non-toxic chemical entity in sub-acute toxicitystudies.
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Aditya Anand,Sarada Prasanna Mallick,Bhisham Narayan Singh,Shikha Kumari,Dheerendra Kumar Suman,Satyavrat Tripathi,Divakar Singh,Pradeep Srivastava 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.4
Usage of bioreactors in the field of tissue engineering has played a significant role in enabling a controlled and reproducible change in the formation of damaged tissue on being provided with specific factors. Owing to the scarcity seen in providing sufficient donor organs for transplantation there is a huge requirement for large-scale production of artificial organs. This cannot be achieved by static culturing since it does not provide an invivo three-dimensional (3D) microenvironment therefore tissue engineering plays a vital role in the development of artificial tissues and organs as per the clinical demands whereas bioreactors have served a major role in providing the artificial microenvironment required by the cells to grow further into a tissue and then into an organ. By providing the specific biochemical cues and mechanoresponsive stimuli the bioreactors turn to be very effective in generating transplantable organs. Apart from performing studies in a controlled manner aimed at understanding biological and physicochemical effects, bioreactors also ensure the safe and reproducible production of tissueengineered constructs to achieve cost-effective large-scale production. The design criteria for bioreactors to be used in tissue engineering include optimal aspect ratio, proper aeration for the cells to proliferate, and agitation with reduced shear stress. The current review summarizes important aspects like Height/Diameter ratio or aspect ratio, shear stress, mechanical stress, aeration, agitation, oxygenation, etc. related to the design of tissue bioreactors, different types of bioreactors that are in use to date, and the reported pieces of literature to yield an overview on the existing concepts. It mainly focuses on the generation of 3D tissue constructs in various reactor systems specially designed for their culture and development along with their applications.
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