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- 저자 : Shijia Liu (검색결과 4 건)
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( Shijia Liu ),( Quji Luorong ),( Kaizhi Hu ),( Weiguo Cao ),( Wei Tao ),( Handeng Liu ),( Dan Zhang ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.9
With changes in human dietary patterns, the proportion of high-fat and high-cholesterol foods in the daily diet has increased. As a result, the incidence rate of cholelithiasis is increasing rapidly. Many studies have reported on the crucial role that the intestinal microflora plays in the progression of gallstones. Although the whole herb of Lysimachia christinae, a traditional Chinese medicine, has long been extensively used as a remedy for cholelithiasis in China, its effects on the intestinal microflora remain unknown. Hence, in this study, we investigated the ability of the aqueous extract of L. christinae (LAE) to prevent cholesterol gallstones (CGSs) in model animals by affecting the intestinal microflora. The effects of LAE on body weight, serum lipid profile, visceral organ indexes, and histomorphology were studied in male C57BL/6J mice, which were induced by a lithogenic diet. After the 8-week study, CGSs formation was greatly reduced after LAE treatment. LAE also reduced body weight gain and hyperlipidemia and restored the histomorphological changes. Moreover, the intestinal microflora exhibited significant variation. In the model group fed the lithogenic diet, the abundances of the genera unclassified Porphyromonadaceae, Lactobacillus and Alloprevotella decreased, but in contrast, Akkermansia dramatically increased compared with the control check group, which was fed a normal diet; the administration of LAE reversed these changes. These results imply that L. christinae can be considered an efficient therapy for eliminating CGSs induced by a high-fat and high-cholesterol diet, which may be achieved by influencing the intestinal microflora.
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Genetic dissection of leaf-related traits using 156 chromosomal segment substitution lines
Xi Liu,Linglong Liu,Yinhui Xiao,Shijia Liu,Yunlu Tian,Liangming Chen,Zhiquan Wang,Ling Jiang,Zhigang Zhao,Jianmin Wan 한국식물학회 2015 Journal of Plant Biology Vol.58 No.6
A two-line super-hybrid rice (Oryza sativa L.) variety [Liangyoupei9 (LYP9)] demonstrated superiority over its both parents, viz. elite inbred lines 93-11 and Pei-ai64S (PA64S), as well as other conventional hybrids, and had long been exploited in China. However, the genetic basis of its leaf-related traits, supposed to be an important component for yield potential, remains elusive. Here, initially a set of chromosome segment substitution lines (CSSLs) was constructed, in which the genome of Pei-ai64S has been introgressed into the background of 93-11. This set was developed by marker aided selection, based on 123 polymorphic SSR markers. The introgressed chromosomal segments presented in the 156 CSSLs covered 96.46% of Pei-ai64S genome. Afterwards, the CSSLs were deployed to assess the genetic basis of leaf size (length and width) and chlorophyll content of top three leaves across five different environments. The CSSLs showed transgressive segregation for all of the traits, and significant correlations were detected among most of the traits. A total of 27 quantitative trait loci (QTL) were identified on ten chromosomes, and three QTL cluster affecting related traits were found on chromosome 3, 6, and 8, respectively. Remarkably, two key QTLs, qALW3-1 and qALW3-2, both controlling the antepenultimate leaf width, were identified in all five environments, and their effect were further validated by CSSLs harboring the two QTL alleles. Our results indicate that developing CSSLs is a powerful tool for genetic dissection of quantitative traits. Meanwhile, the QTLs controlling leaf-related traits uncovered here provide useful information for marker-assisted selection in improving the performance of leaf morphology and photosynthetic ability.
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Wuhua Long,Bangning Dong,Yihua Wang,Pengyi Pan,Yunlong Wang,Linglong Liu,Xiaoli Chen,Xi Liu,Shijia Liu,Yunlu Tian,Liangming Chen,Jianmin Wan 한국식물학회 2017 Journal of Plant Biology Vol.60 No.5
Cereal opaque-kernel mutants are ideal geneticmaterials for studying the mechanism of starch biosynthesisand amyloplast development. Here we isolated and identifiedtwo allelic floury endosperm 8 (flo8) mutants of rice, namedflo8-1 and flo8-2. In the flo8 mutant, the starch content wasdecreased and the normal physicochemical features ofstarch were altered. Map-based cloning and subsequentDNA sequencing analysis revealed a single nucleotidesubstitution and an 8-bp insertion occurred in UDP-glucosepyrophosphorylase 1 (Ugp1) gene in flo8-1 and flo8-2,respectively. Complementation of the flo8-1 mutant restorednormal seed appearance by expressing full length codingsequence of Ugp1. RT-qPCR analysis revealed that Ugp1was ubiquitously expressed. Mutation caused the decreasedUGPase activity and affected the expression of most of genesassociated with starch biosynthesis. Meanwhile, western blotand enzyme activity analyses showed the comparability ofprotein levels and enzyme activity of most tested starchbiosynthesis related genes. Our results demonstrate thatUgp1 plays an important role for starch biosynthesis in riceendosperm.
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