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- 저자 : Shengnan Zhou (검색결과 3 건)
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The bearing capacity of monolithic composite beams with laminated slab throughout fire process
Junli Lyu,Shengnan Zhou,Qichao Chen,Yong Wang 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.38 No.1
To investigate the failure form, bending stiffness, and residual bearing capacity of monolithic composite beams with laminated slab throughout the fire process, fire tests of four monolithic composite beams with laminated slab were performed under constant load and temperature increase. Different factors such as post-pouring layer thickness, lap length of the prefabricated bottom slab, and stud spacing were considered in the fire test. The test results demonstrate that, under the same fire time and external load, the post-pouring layer thickness and stud spacing are important parameters that affect the fire resistance of monolithic composite beams with laminated slab. Similarly, the post-pouring layer thickness and stud spacing are the predominant factors affecting the bending stiffness of monolithic composite beams with laminated slab after fire exposure. The failure forms of monolithic composite beams with laminated slab after the fire are approximately the same as those at room temperature. In both cases, the beams underwent bending failure. However, after exposure to the high-temperature fire, cracks appeared earlier in the monolithic composite beams with laminated slab, and both the residual bearing capacity and bending stiffness were reduced by varying degrees. In this test, the bending bearing capacity and ductility of monolithic composite beams with laminated slab after fire exposure were reduced by 23.3% and 55.4%, respectively, compared with those tested at room temperature. Calculation methods for the residual bearing capacity and bending stiffness of monolithic composite beams with laminated slab in and after the fire are proposed, which demonstrated good accuracy.
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Jingqi Zhang,Jianxing Zhou,Shengnan Wang,Haiwei Wang,Feng-xia Lu 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.9
In order to solve the problem of radiation noise of helicopter reducer with symmetrical structure, a simulation method is proposed to predict the noise of gearbox of a helicopter reducer from steady dynamic response to the acoustic radiation calculation. Primarily, taking the influence of time-varying stiffness into consideration, the lumped mass model of gear-rotorbearing system is established. The dynamic load of transmission system is solved by NewMark-β method, which is regarded as excitation of the gearbox. Then finite element model of housing is established, the radiated noise of the housing is predict by finite element and boundary element method, the influence of multi-source excitation on the radiated noise of the gearbox is analyzed. Finally, the radiated noise of the reducer under different working conditions is explored. All conclusions of this research could provide theoretical reference for vibration and noise reduction of helicopter reducer.
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Mudalal Mahmoud,Wang Zhanqi,Mustafa Shockry,Liu Yiping,Wang Yao,Yu Jize,Wang Shengnan,Sun Xiaolin,Zhou Yanmin 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.5
Background: An in vitro study on rapid culturing method of human gingival fibroblast cells (HGFCs) was established to investigate the potential use of the leukocyte-platelet rich fibrin (L-PRF) in tissue engineering technology, different medical fields, including periodontology and implantology. Methods: Eight biopsies were obtained from eight different donors and a modified culturing technique was developed to obtain HGFCs. The modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay was used to compare the cell viability when the modified culturing method was used in comparison to the standard method. Blood samples were collected from the same patients and L-PRF was isolated using a standard protocol. The releases of platelet-derived growth factor-AA and transforming growth factor-beta1 at various time intervals were observed using enzyme-linked immunosorbent assay (ELISA) kit. The proliferative effect of L-PRF on HGFCs was assessed by the cell counting kit—8 assay. Results: A simple and rapid modified method for in vitro HGFC culture yielded a cellular monolayer within three to nine days after cell culture. L-PRF with three-dimensional polymer fibers released growth factors that peaked during the first three hours and continued to produce up to 10 days. The L-PRF presented a dose-dependent effect on HGFCs proliferation where HGFCs proliferation increased with an increase in L-PRF concentration. Conclusion: The modified technique for the culture of HGFCs might be useful for the development of future experimental and clinical studies, besides L-PRF has great therapeutic potential in oral surgery fields. Background: An in vitro study on rapid culturing method of human gingival fibroblast cells (HGFCs) was established to investigate the potential use of the leukocyte-platelet rich fibrin (L-PRF) in tissue engineering technology, different medical fields, including periodontology and implantology. Methods: Eight biopsies were obtained from eight different donors and a modified culturing technique was developed to obtain HGFCs. The modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay was used to compare the cell viability when the modified culturing method was used in comparison to the standard method. Blood samples were collected from the same patients and L-PRF was isolated using a standard protocol. The releases of platelet-derived growth factor-AA and transforming growth factor-beta1 at various time intervals were observed using enzyme-linked immunosorbent assay (ELISA) kit. The proliferative effect of L-PRF on HGFCs was assessed by the cell counting kit—8 assay. Results: A simple and rapid modified method for in vitro HGFC culture yielded a cellular monolayer within three to nine days after cell culture. L-PRF with three-dimensional polymer fibers released growth factors that peaked during the first three hours and continued to produce up to 10 days. The L-PRF presented a dose-dependent effect on HGFCs proliferation where HGFCs proliferation increased with an increase in L-PRF concentration. Conclusion: The modified technique for the culture of HGFCs might be useful for the development of future experimental and clinical studies, besides L-PRF has great therapeutic potential in oral surgery fields.
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