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Shaaban, Mona I.,Abdelmegeed, Eman,Ali, Youssif M. The Korean Society for Microbiology and Biotechnol 2015 Journal of microbiology and biotechnology Vol.25 No.6
Uricase is an important microbial enzyme that can be used in the clinical treatment of gout, hyperuricemia, and tumor lysis syndrome. A total of 127 clinical isolates of Pseudomonas aeruginosa were tested for uricase production. A Pseudomonas strain named Ps43 showed the highest level of native uricase enzyme expression. The open reading frame of the uricase enzyme was amplified from Ps43 and cloned into the expression vector pRSET-B. Uricase was expressed using E. coli BL21 (DE3). The ORF was sequenced and assigned GenBank Accession No. KJ718888. The nucleotide sequence analysis was identical to the coding sequence of uricase gene puuDof P. aeruginosa PAO1. We report the successful expression of P. aeruginosa uricase in Escherichia coli. E. coli showed an induced protein with a molecular mass of about 58 kDa that was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting. We also established efficient protein purification using the Ni-Sepharose column with activity of the purified enzyme of 2.16 IU and a 2-fold increase in the specific activity of the pure enzyme compared with the crude enzyme.
Attitude Dynamics Identification of Unmanned Aircraft Vehicle
Shaaban Ali Salman,Anavatti G. Sreenatha,Jin Young Choi 대한전기학회 2006 International Journal of Control, Automation, and Vol.4 No.6
The role of Unmanned Aircraft Vehicles (UAVs) has been increasing significantly in both military and civilian operations. Many complex systems, such as UAVs, are difficult to model accurately because they exhibit nonlinearity and show variations with time. Therefore, the control system must address the issues of uncertainty, nonlinearity, and complexity. Hence, identification of the mathematical model is an important process in controller design. In this paper, attitude dynamics identification of UAV is investigated. Using the flight data, nonlinear state space model for attitude dynamics of UAV is derived and verified. Real time simulation results show that the model dynamics match experimental data.
The Role of Augmented Reality in Solving Abstract Concepts Teaching Problems: A review article
Sayed Shaaban Abd-ul Aliem Younes International Journal of Computer ScienceNetwork S 2023 International journal of computer science and netw Vol.23 No.2
To find research that concentrated on the use of augmented reality to address issues with teaching abstract concepts, several international databases were examined. Thirty research, published between the years (2013-2022), were analyzed to determine the many functions of augmented reality in resolving issues with abstract notions. These studies discovered numerous roles, which are listed below. The use of augmented reality in the following areas: the simplification of abstract ideas, the embodiment of abstract ideas, the enrichment of examples of abstract ideas, the resolution of conceptual misunderstandings, the stimulation of interest in learning abstract ideas, and more, and the research ended with the presentation of the conclusions.
Khaled Shaaban,Deepti Muley,Dina Elnashar 서울시립대학교 도시과학연구원 2018 도시과학국제저널 Vol.22 No.3
The city of Doha, Qatar experiences a hot and arid weather throughout the year. This study investigates the effect of season, type of day, and time of day on the walking behaviour of pedestrians in these conditions using logistic regression. The study uses data obtained from observational surveys conducted at a densely populated mixed-use neighbourhood located in the heart of the city. The results showed the walking activity is much less during hot weather. The results of the logistic regression analysis suggested that the pedestrians from other nationalities living in Qatar had much higher odds of walking compared to the Qatari nationals. Furthermore, men had almost double the odds of walking compared to females. The choice of the time of day model indicated that a pedestrian has more than twice the odds of walking in the evening time compared to the morning and afternoon times. The results can help policy makers and public agencies to develop programmes to promote the walking culture in this region.
CRASHWORTHINESS OPTIMIZATION OF IMPACT ATTENUATORS CONSTRUCTED OF POLYURETHANE FOAM
Amr Shaaban,Adel Moneb Elsabbagh 한국자동차공학회 2022 International journal of automotive technology Vol.23 No.2
This paper examines the crashworthiness optimization of an impact attenuator constructed of polyurethane (PUR) foam used in racing vehicles. Different design variables are investigated such as the mechanical properties associated with each PUR density and the attenuator topology. Analytical method is employed to model the behavior of the PUR, while finite element (FE)-simulation using LS-Dyna4.3© is conducted to evaluate the performance of the attenuator. The evaluation criteria in this study are the average and maximum acceleration throughout the impact period. The FE-results reveal that the PUR of 80 kg/m3 is the most suitable, and experimental test is conducted for verification. The design of the attenuator is then modified by adding an internal cavity to provide a homogeneous cross-sectional area along the attenuator length. Size optimization analysis is carried out to attain stable acceleration values with the least average. Two approaches are considered for the design of the internal cavity, namely constant thickness and varied thickness. Various designs, each of specific cavity size and PUR density, are tested and the results are presented against each other. The varied thickness design with 30 mm base thickness and 145 kg/m3 has proved to be the optimum design.
Integration of Energy Storage for Voltage Support in Distribution Systems with PV Generation
Mohamed Shaaban,J .O. Petinrin 대한전기학회 2016 Journal of Electrical Engineering & Technology Vol.11 No.5
The exploitation of PV generation offers substantial environmental benefits. Yet, the integration of these might be problematic due to its variable and intermittent nature. Energy storage can compensate for the deficiencies featured by variable PV systems. This paper provides a mathematical programming based approach for the strategic deployment of energy storage in the feeder circuits to achieve ubiquitous grid benefits. A genetic algorithm (GA) is used to solve the problem of appropriate size and location of the energy storage. Quasi-static time sequence analysis is carried out on diurnal basis, to correlate the coincidence of the PV resource output and demand. This will ensure accurate appraisal of storage impacts on the system hour by hour. The proposed approach is implemented on the IEEE 123 node test feeder under various penetration levels and operating scenarios. Results have consistently affirmed that the proposed approach is efficient in maintaining system’s voltage profile at various conditions as well as reducing the overall thermal losses.