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Using EMG Sensor WooridulChair Usability Evaluation
Da Seu Ran Kim,Sung Koon Lee,Peom Park 대한인간공학회 2011 대한인간공학회 학술대회논문집 Vol.2011 No.5
Background: Recently, modern people most of the time learning or office work is carried out sitting in a chair and neck, shoulder, back and waist pain on people are increasing by the upper body fatigue that comes from a long time incomplete position. Objective: Therefore in this study, when office work and learning activities time for the prevention of musculoskeletal disorders, on the chest support of the chair and stool for the existing process through usability testing is to verify the effectiveness. Method: Especially, progressed measurement that use EMG sensor and surveyed to evaluate fatigue degree of reagent's upper body main region simultaneously. The 20~27 year-old college students subject, 6 or more hours daily use chairs, 1 hour in a given situation, neck, shoulders, back and waist were measured at the EMG. Results: In the case of experiment result chest support chair, keep attitude right relatively, can know that fatigue degree of muscle appears to be less than general chair. Conclusion: Through this study, the chest support chair's effects for the maintenance of good posture and the could be verified, of the future, development of office chairs is expected to have a positive impact.
Stimulation of Cell Growth by Erythropoietin in RAW264.7 Cells: Association with AP-1 Activation
Seong Seu-Run,Lee Jae-Woong,Lee Yong-Kyoung,Kim Tae-Il,Son Dong-Ju,Moon Dong-Cheol,Yun Young-Won,Yoon Do-Young,Hong Jin-Tae The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.3
Erythropoietin (EPO), a hematopoietic factor, is required for normal erythrocyte developments, but it has been demonstrated to have many other functions, and its receptor is localized in other tissues. In the present study, we investigated whether EPO can promote other cell proliferation and possible molecular mechanisms. EPO restored the inhibition of the RAW264.7 and PC12 cell growth by fetal bovine serum (FBS) withdrawal in a dose dependent manner, but not that of other cell types tested. The restoring effect of EPO was completed when the RAW264.7 cells were cultured in the medium containing as low as 3% of FBS, and 10 U/mL EPO could replace FBS. The restoring effect of EPO in the RAW264.7 cells was associated with the increased of c-Fos and c-Jun expression as well as AP-1 activation. These data demonstrate that EPO can stimulate RAW264. 7 cell as well as PC12 cell growth even when the cells were cultured without FBS or in the presence of small amounts of FBS in the medium, and this stimulating effect is associated with the activation of AP-1 transcription factor.
Kim, Young Ho,Lee, Jae Ran,Seu, Jung Hwn 한국미생물 · 생명공학회 1993 Journal of microbiology and biotechnology Vol.3 No.4
A strain of yeast which can convert starch directly to ethanol was developed by the intergeneric protoplast fusion between Schwanniomyces alluvius possessing a amylase as well as glucoamylase with debranching activity and FSC-14-75 which previously had been formed from a heterologous transformation and subsequent intergeneric protoplast fusion. Fusants were selected on minimal medium after protoplasts of auxotrophic mutant of S. alluvius fused with heat-treated protoplasts of FSC-14-75 in the presence of 30%(w/v) PEG and 20mM CaCl_2. The fusion frequency was in the range of 10^-6 order. All fusants tested were intermediate types of parental strains for carbon compound assimilation, and their cell volumes were approximately 1.1 times larger than FSC-14-75 and 1.8 times larger than S. alluvius. The fusants were unable to sporulate like FSC-14-75, while S. alluvius could sporulate. In flask scale the most promising fusant, FSCSa-R10-6, produced 7.83%(v/v) and 10.17%(v/v) ethanol from 15% and 20% of liquefied potato starch, respectively, indicating that the fermetation efficiency of each case increased 1.2 times and 1.6 times than that of FSC-14-75. The elution pattern on DEAE-cellulose chromatography showed that FSCSa-R10-6 has four distinct amylase peaks of which two peaks originated from S. alluvius and the other two from FSC-14-75. These results suggest that the enhanced fermentation efficiency of the fusant might be due to almost-complemented parental amylases.