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        A Seedbrone Fungus Bipolaris spicifera Deteced from Imported Grass Seeds

        구한모,정일민,Sang-HunLee,Se-ChulChun 한국식물병리학회 2003 Plant Pathology Journal Vol.19 No.3

        Seedborne fungus Bipolaris spicifera, which has not been previously reported in Korea, was detected from imported grass seeds in the country. The most frequently detected fungi from the seeds were Fusarium species, Ulocladium atrum, B. spicifera, Alternaria, and Cuvularia lunata among 17 different seed samples of the family Gramineae. Detection frequencies of B. spicifera were 11, 8, 5% in Bermuda grass, tall fescue, and mixed lawn grass imported from USA, respectively, and 9% in mixed lawn grass imported from Italy. This suggests that important seedborne pathogen could be spread between countries through seed sources. The pathogen was seed-transmitted causing damping-off of Bermuda grass seedlings and showed strong pathogenicity to rice, corn, Bermuda grass, sorghum, and tall fescue. However, it did not infect wheat and blue grass.

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        Characterization of Cell Wall Proteins from the soo1-1/ret1-1 Mutant of Saccharomyces cerevisiae

        Dong-WonLee,Ki-HyunKim,Se-ChulChun,Hee-MoonPark 한국미생물학회 2002 The journal of microbiology Vol.40 No.3

        In order to investigate the function of Soo1p/α-COP during post-translational modification and intracellular transport of cell wall proteins in Saccharomyces cerevisiae, cell wall proteins from the soo1-1/ ret1-1 mutant cells were analyzed. SDS-PAGE analysis of biotin labeled cell wall proteins suggested that the soo1-1 mutation impairs post-translational modification of cell wall proteins, such as N- and/ or O-glycosylation. Analysis of cell wall proteins with antibodies against β-1,3-glucan and β-1,6-glucan revealed alteration of the linkage between cell wall proteins and β-glucans in the soo1-1 mutant cells. Compositional sugar analysis of the cell wall proteins also suggested that the soo1-1 mutation impairs glycosylation of cell wall protein in the ER, which is crucial for the maintenance of cell wall integrity.

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