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RISS 인기검색어
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- 저자 : Sariah Meon (검색결과 3 건)
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Shamala Sundram,Sariah Meon,Idris Abu Seman,Radziah Othman 한국미생물학회 2011 The journal of microbiology Vol.49 No.4
Endophytic bacteria (Pseudomonas aeruginosa UPMP3 and Burkholderia cepacia UPMB3), isolated from within roots of oil palm (Elaeis guineensis Jacq.), were tested for their presymbiotic effects on two arbuscular mycorrhizal fungi, (Glomus intraradices UT126 and Glomus clarum BR152B). These endophytic bacteria were also tested for antagonistic effects on Ganoderma boninense PER 71, a white wood rot fungal pathogen that causes a serious disease in oil palm. Spore germination and hyphal length of each arbuscular mycorrhizal fungal (AMF) pairing with endophytic bacteria was found to be significantly higher than spores plated in the absence of bacteria. Scanning electron microscopy (SEM) showed that the endophytic bacteria were scattered, resting or embedded on the surface hyaline layer or on the degraded walls of AMF spores, possibly feeding on the outer hyaline spore wall. The antagonistic effect of the endophytic bacteria was expressed as severe morphological abnormalities in the hyphal structures of G. boninense PER 71. The effects of the endophytic bacteria on G. boninense PER 71 hyphal structures were observed clearly under SEM. Severe inter-twisting, distortion, lysis and shrivelling of the hyphal structures were observed. This study found that the effect of endophytic bacteria on G. intraradices UT126 and G. clarum BR152B resembled that of a mycorrhiza helper bacteria (MHB) association because the association significantly promoted AMF spore germination and hyphal length. However, the endophytic bacteria were extremely damaging to G. boninense PER 71.
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Bidabadi, Siamak Shirani,Mahmood, Maziah,Meon, Sariah,Wahab, Zakaria,Ghobadi, Cyrus 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.4
Water stress is a serious environmental restriction to banana productivity. Hence, the objective of this study was to employ in vitro mutagenesis in selection and characterization of drought tolerant lines in banana. In vitro culture responses of ethyl methanesulphonate induced variants of banana cultivars, 'Berangan Intan' and 'Berangan' were assessed concerning morphological, physiological and molecular characteristics involving mutated shoot tips on MS medium supplemented with 30 g $L^{-1}$ PEG. The results showed that water stress tolerant lines could be obtained from induced variations. Variants $L_{2-5}$ and $L_{1-5}$ showed the highest number of leaves per shoot (2.37 and 2.06, respectively) and the lowest were recorded in the parental lines $L_{1-1}$ and $L_{2-1}$ (0.81 and 0.93, respectively). Fresh weight and shoot vigor rate indicated the maximum increase in the water stress tolerant lines compared with susceptible and non-mutated parental lines. $L_{2-5}$ exhibited the most increase in the chlorophyll and the most reduction in $H_2O_2$ and MDA contents when exposed to water stress. Under PEG treatment, proline and relative water content was enhanced in $L_{1-5}$, $L_{2-5}$, $L_{2-6}$, $L_{1-6}$, $L_{2-3}$, $L_{2-4}$, and $L_{1-4}$. RAPD analysis revealed polymorphism (18.35 and 21.48%) among variants derived from 'Berangan Intan' and 'Berangan', respectively. The amplified fragments generated by primers opc01, opc04, opa11, and opa20 observed to be specific for $L_{2-5}$ and $L_{1-5}$ as more tolerant followed by $L_{2-3}$, $L_{1-4}$, $L_{2-6}$, and $L_{1-6}$ as moderately tolerant lines against water stress. This study demonstrates the application of in vitro mutagenesis in selection of water stress tolerant lines of banana as a convenient, cheap, and rapid technique.
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Siamak Shirani Bidabadi,Maziah Mahmood,Sariah Meon,Zakaria Wahab,Cyrus Ghobadi 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.4
Water stress is a serious environmental restriction to banana productivity. Hence, the objective of this study was to employ in vitro mutagenesis in selection and characterization of drought tolerant lines in banana. In vitro culture responses of ethyl methanesulphonate induced variants of banana cultivars, ‘Berangan Intan’ and ‘Berangan’ were assessed concerning morphological, physiological and molecular characteristics involving mutated shoot tips on MS medium supplemented with 30 g L_(-1) PEG. The results showed that water stress tolerant lines could be obtained from induced variations. Variants L_(2-5) and L_(1-5) showed the highest number of leaves per shoot (2.37 and 2.06, respectively) and the lowest were recorded in the parental lines L_(1-1) and L_(2-1) (0.81 and 0.93, respectively). Fresh weight and shoot vigor rate indicated the maximum increase in the water stress tolerant lines compared with susceptible and non-mutated parental lines. L_(2-5) exhibited the most increase in the chlorophyll and the most reduction in H₂O₂ and MDA contents when exposed to water stress. Under PEG treatment, proline and relative water content was enhanced in L_(1-5), L_(2-5), L_(2-6), L_(1-6) L_(2-3), L_(2-4), and L_(1-4). RAPD analysis revealed polymorphism (18.35 and 21.48%) among variants derived from ‘Berangan Intan’ and ‘Berangan’,respectively. The amplified fragments generated by primers opc01, opc04, opa11, and opa20 observed to be specific for L_(2-5) and L_(1-5) as more tolerant followed by L_(2-3), L_(1-4), L_(2-6), and L_(1-6) as moderately tolerant lines against water stress. This study demonstrates the application of in vitro mutagenesis in selection of water stress tolerant lines of banana as a convenient, cheap, and rapid technique.
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