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Santosh Kumar Shukla,Suman Bala Sharma,Usha Rani Singh,Sayeed Ahmad,Ankur Maheshwari,Manmohan Misro,Shridhar Dwivedi 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.2
Preventive effects of hydroalcoholic extract of fruit pulp of Eugenia jambolana (HEEJ) on isoproterenol (ISP)-induced myocardial damage in rats were evaluated. Rats were pre-treated with HEEJ (100, 200, and 400 mg/kg) daily for 30 days. ISP (85 mg/kg bw) was administered on the 28th and 29th days at an interval of 24 h. Ischemic control group exhibited significant increases in oxidative stress parameters, markers of inflammation, cardiac damage markers, and apoptotic markers. Oral pre-treatment with HEEJ (100, 200, and 400 mg/kg bw) provided cardioprotective activity by decreasing levels of malondialdehyde, cardiac markers (serum glutamate oxaloacetate transaminase, creatine kinase-myocardial band, cardiac troponin I), and markers of inflammation (interleukin-6, C-reactive protein, and tumor necrosis factor alpha); and increased levels of superoxide dismutase and reduced glutathione. HEEJ (400 mg/kg bw) was found to exert significantly greater effects in comparison to HEEJ (100 and 200 mg/kg bw). Apoptotic marker Bcl-2 was increased, while Bax was decreased in pretreated rats, which was further confirmed by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay. The present study provides evidence that pre-treatment with HEEJ attenuates oxidative stress, apoptosis and improves cardiac architecture in ISP-induced rats and, hence, is cardioprotective.
In vitro and In vivo Biocompatibility Evaluation of Freeze Dried Gelatin Haemostat
Sneha Letha S,Santosh Kumar Shukla,Neena Haridas,Smitha R P,Sidharth Mohan M,Archana V,Rosemary M J 한국섬유공학회 2021 Fibers and polymers Vol.22 No.3
Gelatin sponges developed by crosslinking gelatin with formaldehyde followed by controlled freeze-drying resultsin soft, spongy porous structures, which are useful in arresting bleeding during surgical procedures. The aim of this article isto conduct the in vivo evaluation of the gelatin sponge as per ISO 10993: Biological evaluation of medical devices; and proveits biocompatibility. The sponges were also evaluated by morphology analysis, tensile strength and blood-absorption studies. Sponges were found to be microporous with considerable mechanical strength to withstand any rupture during its application. Blood absorption studies showed good absorption behaviour. As per ISO 10993; cytotoxicity, skin irritation, sensitisation andblood compatibly studies has to be conducted to check the biocompatibility of a biomaterial. Gelatin sponge was found to benon-cytotoxic in Balb/c 3T3 cells. The experiments conducted on albino rabbits and guinea pig concluded that the materialdoes not cause any irritation and sensitisation in vivo and is non-haemolytic when in contact with blood. Hence the materialmeets the requirements of a biocompatible haemostatic agent for the management of blood loss during surgical procedures.