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Expression of deafness protein Tmie in postnatal developmental stages of C57BL/6J mice
Sankarapandian Karuppasamy,YoonYi Nam,Harry Jung,Jun-Gyo Suh 한국실험동물학회 2012 Laboratory Animal Research Vol.28 No.2
Loss-of function mutations in the transmembrane inner ear expressed (Tmie/TMIE) gene have been shown to cause deafness in mice and humans (DFNB6). Previous studies report that the circling mouse can be an animal model for DFNB6. However, the expression pattern of Tmie protein in postnatal developmental stages has not been clearly revealed. In this study we tried to investigate the expression of Tmie protein in the liver, spleen, kidney, and lung, as well as in the cochlea. We examined various tissue samples from five different age groups of C57BL/6J animals. Using western blotting analysis, the expression of Tmie protein in these organs has been identified. The results show that Tmie protein expression in the cochlea has been increased in postnatal developmental stages, indicating that Tmie plays an important role in not only the development and also in the function of the cochlea. The expression pattern of Tmie in adult mouse organs such as the liver, spleen, kidney, and spleen significantly vary in adult rats. The order of Tmie expression level in mice (63 days after birth) was spleen, liver, lung, cochlea, and kidney, whereas in the adult rat it was liver, cochlea, lung, spleen, and kidney.
Sankarapandian Karuppasamy,Yoon Yi Nam,Harry Jun,Byoungkwon Park,Hyung-Joo Kwon,Jun-Gyo Suh 한국실험동물학회 2011 Laboratory Animal Research Vol.27 No.4
Mutations in the transmembrane inner ear (Tmie) gene, which encodes the Tmie protein, have been attributed to deafness autosomal recessive 6 (DFNB6), an autosomal nonsyndromic recessive hearing loss disorder. Although the Tmie gene was identified a few years ago, little is known about subcellular localization of the Tmie protein. In order to address this, we developed a stable cell line expressing Tmie protein. The expression of Myc-tagged Tmie protein was confirmed by Western blot analysis using an anti-Myc antibody and localization of the Tmie protein was confirmed by immunostaining, using the anti-Myc antibody as well as the anti-tmie antibody. Our study demonstrates that the Tmie protein is localized mostly in the cellular membrane and to a lesser extent in cytoplasm. These results suggest that our Tmie expressing stable cell line provides a suitable in vitro model to explore Tmie synthesis and functions.
Xue Wang,Karuppasamy Sankarapandian,Yizhe Cheng,Soon Ok Woo,Hyung Wook Kwon,Haribalan Perumalsamy,Young-Joon Ahn 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10
Propolis collected from botanical sources by honey bee, has been used as a popular natural remedies in folk medicine throughout the world. This study was conducted to assess growth inhibitory effects of ethanol extracts of propolis (EEPs) from 20 different regions in South Korea on human intestinal bacteria as well as BACE-1, AChE inhibitory, antioxidant, antiproliferative, and anti-human rhinovirus activities. Correlation coefficient (r) analysis of the biological activities of EEP samples was determined using their 50% inhibition concentration or minimal inhibitory concentration values and their polyphenol or flavonoid contents in 20 Korean EEP samples. Correlation coefficient analysis showed that total polyphenol contents may be negatively correlated with DPPH free radical scavenging activity (r = –0.872) and total flavonoid content has no correlation with the activity (r = 0.071). No direct correlation between BACE-1 inhibition, AChE inhibition, or antiproliferative activity and total polyphenol or total flavonoid content in Korean EEP samples was found.
Perumalsamy, Haribalan,Sankarapandian, Karuppasamy,Veerappan, Karpagam,Natarajan, Sathishkumar,Kandaswamy, Narendran,Thangavelu, Lakshmi,Balusamy, Sri Renukadevi Elsevier 2018 Phytomedicine Vol.46 No.-
<P>Conclusion: Our study denotes that SSBC could be very effective against AGS by inducing apoptosis through intrinsic pathway and recommended for in vivo and human trials.</P>
Production and Characterization of Polyclonal Antibody to Transmembrane Inner Ear Protein
YoonYi Nam,Sankarapandian Karuppasamy,Byoungkwon Park,Hyung-Joo Kwon,Jun-Gyo Suh 한국실험동물학회 2009 Laboratory Animal Research Vol.25 No.2
Deafness is one of the most common sensory deficits in human. Since the inner ears of mice and humans are anatomically and physiologically similar, mouse mutants allow us to reveal detailed analysis of defects in the cochlea and vestibular apparatus. Loss of function mutations in transmembrane inner ear (Tmie) protein has been shown to cause deafness in mice (circling, spinner) and humans (DFNB6). However, the detailed mechanism of Tmie protein in the cochlea still remains unclear. First step to achieve that purpose, we produced polyclonal antibody that recognize Tmie protein. The specifically selected amino acid sequence localized at the positions 118-133 (IPGEDKKKKKKDSVD) of Tmie protein has been used as immunogen to produce rabbit polyclonal antibody. Two rabbits were immunized with polypeptide and the serum was harvested from the rabbits served as the source of the polyclonal antibody specific to Tmie. Specificity of the antibody was determined by Western blot analysis. After transfection of tmiePC/pcDNA vector in HEK cells, we identified a specific band of tmie protein. Taken together, our anti-Tmie antibody can be useful for studying localization and characterization of Tmie protein.
Haribalan Perumalsamy,Karuppasamy Sankarapandian,Xue Wang,Hye Min Shin,Young Joon Ahn 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.10
The American house dust mite, Dermatophagoides farinae Hughes, 1961 (Acari: Pyroglyphidae), is recognized as an important source of allergens in the domestic environment. An assessment was made of the toxicity of constituents from essential oil of cade, Juniperus oxycedrus L. (Cupressaceae), and related compounds as well as four experimental spray formulations containing the oil (10–40 g/liter sprays) to adult D. farinae. In a contact + fumigant mortality bioassay, methyleugenol (24-h LC50,5.82 μg/cm2) and guaiacol (8.24 μg/cm2) were the most toxic constituents to the mites and the toxicity of these constituents and benzyl benzoate did not differ significantly.