http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Jia-Ying Zhu,Sang-Zi Ze,Bin Yang 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.1
Chemosensory proteins (CSPs) are believed to be important in the detection of semiochemicals, and have been reported to be involved in development, molting, leg regeneration and phase transition. In the current study, six CSP genes were identified in the beet armyworm Spodoptera exigua, namely SexiCSP1-6, from the full length cDNA library.Multiple alignment and phylogenetic analysis indicated that theywere very diverse as seen in CSPs of other insect species. Expression patterns determined by quantitative real-time PCR (qPCR) revealed that they were highly expressed in antenna. SexiCSP1-3, SexiCSP5 and SexiCSP6 were male antenna-biased, while SexiCSP4 was female-biased in the antenna. In addition to antenna, they were expressed at high levels in legs and wings, suggesting potential roles in contact chemoreception and non-olfactory roles. Compared with the expression in antenna, legs and wings, only SexiCSP1 showed relatively high expression in heads, thoraxes and abdomen. These results extended olfactory gene resource in the beet armyworm and provided basis for further gaining a better understanding of CSPs roles in this pest.
Nai-Yong Liu,Guo-XingWu,Sang-Zi Ze,Bin Yang,Jia-Ying Zhu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
The genital systems of insects are undoubtedly of great key for the success of mating and population reproduction. In the meantime,morphological and ultrastructural characteristics of the reproductive systems also provide valuable evidences for the studies of taxonomy, reproductive biology and evolutionary biology.More previously, the reproductive systemof female Nasonia vitripennis was already described. To complement the information of reproductive systems from this species, the male reproductive system was here dissected and characterized by light microscopy and transmission electron microscopy. Results showed that the system follows the pattern of most Hymenoptera species: paired testes with one follicle, deferent ducts, seminal vesicles, accessory glands and a fused ejaculatory duct ultimately connected to the external aedeagus. Histologically, the testes, seminal vesicles and accessory glands are all composed of three portions, all of which include epithelial and luminal regions. In addition, both lipidic inclusions and nuclei are presented in the three organs with variable sizes and shapes. Spermatozoa are observed in the testes and seminal vesicles, and also vary considerably in size and shape, suggesting different phases of spermatogenesis.Mitochondria are abundant in seminal vesicles and accessory glands. Notably, membranous inclusions and Golgi complexes are found only in seminal vesicles, whereas secretory granules are presented only in accessory glands, being indicative of organ or age specificity. Together, this study complements the information of the reproductive systems from N. vitripennis, and provides an extensive resource for taxonomy, reproductive biology and evolutionary biology.
Chemosensory genes from Pachypeltis micranthus, a natural enemy of the climbing hemp vine
Nai-Yong Liu,Jia-Ying Zhu,Mei Ji,Bin Yang,Sang-Zi Ze 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
The plant bug, Pachypeltis micranthus (Hemiptera: Miridae), is a natural enemy of the invasive alien weed Mikania micrantha with a widespread distribution in South China as well as other countries. The interactions of P. micranthus and its host M. micrantha, associated with a linkage of host recognition, are of great importance for its survival and reproduction. The identification of olfactory-related genes is undoubtedly the initially key step, to uncover how P. micranthus perceives and recognizes the specific host plant M. micrantha. Here, we constructed an antennal full length cDNA library from P. micranthus antennae. By sequencing, a total of 13 transcripts encoding chemosensory-related genes were identified, comprising nine odorant-binding proteins (OBPs), three chemosensory proteins (CSPs) and one odorant receptor (OR). All identified OBPs and CSPs shared classic characteristics of conserved cysteines and a signal peptide. Expression profiles by quantitative real time PCR (qPCR) revealed that as many as 12/13 chemosensory genes were expressed predominantly in the antennae at a sex-biased pattern, strongly linking to their specific olfactory functions. Notably, expression of all these genes was also differentially detected in legs of both sexes with an exception of PmicOBP7, indicative of their functional differentiation between female and male adults. Further, molecular docking of PmicOBP6 and CSP1 to behaviorally active compounds provided the potentially key residues for ligand-binding, which deserves further studies.