Dietary Calcium and 1,25-Dihydroxyvitamin D₃ Regulate Transcription of Calcium Transporter Genes in Calbindin-D9k Knockout Mice

KO, Sang-Hwan,LEE, Geun-Shik,VO, Thuy T. B.,JUNG, Eui-Man,CHOI, Kyung-Chul,CHEUNG, Ki-Wha,KIM, Jae Wha,PARK, Jong-Gil,OH, Goo Taeg,JEUNG, Eui-Bae Society for Reproduction and Development 2009 Journal of Reproduction and Development Vol.55 No.2

<P>The effect(s) of oral calcium and vitamin D<SUB>3</SUB> were examined on the expression of duodenal and renal active calcium transport genes, i.e., calbindin-D9k (<I>CaBP-9k</I>) and calbindin-D28k (<I>CaBP-28k</I>), transient receptor potential cation channels (<I>TRPV5</I> and <I>TRPV6</I>), Na<SUP>+</SUP>/Ca<SUP>2+</SUP> exchanger 1 (<I>NCX1</I>) and plasma membrane calcium ATPase 1b (<I>PMCA1b</I>), in <I>CaBP-9k</I> KO mice. Wild-type (WT) and KO mice were provided with calcium and vitamin D<SUB>3</SUB>-deficient diets for 10 weeks. The deficient diet significantly decreased body weights compared with the normal diet groups. The serum calcium concentration of the WT mice was decreased by the deficient diet but was unchanged in the KO mice. The deficient diet significantly increased duodenal transcription of <I>CaBP-9k</I> and <I>TRPV6</I> in the WT mice, but no alteration was observed in the KO mice. In the kidney, the deficient diet significantly increased renal transcripts of <I>CaBP-9k</I>, <I>TRPV6</I>, <I>PMCA1b</I>, <I>CaBP-28k</I> and <I>TRPV5</I> in the WT mice but did not alter calcium-relating genes in the KO mice. Two potential mediators of calcium-processing genes, vitamin D receptor (VDR) and parathyroid hormone receptor (PTHR), have been suggested to be useful for elucidating these differential regulations in the calcium-related genes of the KO mice. Expression of VDR was not significantly affected by diet or the KO mutation. Renal <I>PTHR</I> mRNA levels were reduced by the diet, and reduced expression was also seen in the KO mice given the normal diet. Taken together, these results suggest that the active calcium transporting genes in KO mice may have resistance to the deficiency diet of calcium and vitamin D<SUB>3</SUB>.</P>

The Korean Academy of Tuberculosis and Respiratory Diseases : Slide Session ; OS-104 : Critical Care ; The Amount of Visceral Adipose Tissue Quantitatively Measured by Abdominal Computed Tomography Is Dose-Dependently Associated with Mortality in Patients

( Sung Jun Ko ),( Sun Mi Choi ),( Jin Woo Lee ),( Young Sik Park ),( Chang Hoon Lee ),( Jae Joon Yim ),( Chul Gyu Yoo ),( Young Whan Kim ),( Sung Koo Han ),( Sang Min Lee ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Adipose tissue is recently recognized as not only energy reservoir but also endocrine organ producing proin ammatory cytokines. Especially the impact o visceral adipose tissue(VAT) in critical illnesses has been proposed, but researches on the association between VAT and sepsis were scarce and quantitative measurement of VAT had not been used. Methods: In this retrospective cohort study, we enrolled patients with sepsis who examined abdominal computed tomography(A-CT) within 1 month of occurrence of sepsis, among the patients admitted to intensive care unit(ICU). Age, sex, anthropometric values, comorbidities and APACHE II score were reviewed. The areas of VAT and total adipose tissue(TAT) on the section of A-CT image of the umbilicus level were measured by calculating pixels presenting fat density. Results: Among 287 patients admitted to ICU due to sepsis, 178 patients were included for this study. Median age was 65 and 59.0% were men. In-hospital mortality rate was 57.9%. Women had higher TAT and subcutaneous adipose tissue, lower VAT/TAT ratio compared to men. The amount of VAT and VAT/TAT ratio were higher in in-hospital mortality group than in survivor group(90.41cm2 vs. 63.83cm2 and 45.88% vs. 32.79%, p=0.001 and <0.001, respectively). After adjusting age, sex, APACHE II score and comorbidities, multivariate logistic regression analysis revealed that the amount of VAT and VAT/TAT ratio were independent prognostic factors of sepsis with obvious dose-dependent relationship(VAT/TAT ratio quartile 3: OR 8.832, p<0.001 and quartile 4: OR 29.477, p<0.001, compared to quartile 1 respectively). Conclusion: The amount of VAT and VAT/TAT ratio quantitatively measured by A-CT were positively correlated with mortality in sepsis, and this association was dose-dependent. Visceral obesity should be considered as the poor prognostic factor of sepsis.

EXPLORING THE EFFECTIVENESS OF A COMPLEX COATING TECHNIQUE FOR USE THE SMALLEST PARTS OF ADVANCED POWERTRAIN FUEL SYSTEM

Sung Chul Cha,Heon Joon Park,Jin Han Lee,Kwang Yool Ko,Chang Hyun Shin 한국자동차공학회 2020 International journal of automotive technology Vol.21 No.3

The objective is to develop the complex coating technique with high quality and homogeneity of coating for spherical parts with diameter of 2 ~ 4 mm and stable mass production shall be secured with consideration of coating material, process and jig design. Silicon oxide-diamond like carbon (SiO-DLC) complex coating chemistry is expected to enhance the high temperature stability as opposed to DLC. Currently, the spherical part is first welded and then assembled with other parts, finally they are coated with DLC. However in this manner, the maximum charging in one coating batch is limited due to the very big size of the whole component which increases cost. Therefore the spherical part is to be coated before welding. Overall, reactive sputtering with high precision fixturing and masking technique resulted in the best overall properties for modern powertrain fuel system. The jig design was optimized with consideration of coating properties (height, width, thickness, inserting distance) and spherical parts were fixed at the jig with magnet plate. Coating material and process were determined with several trials. In conclusion the PVD+PACVD coated parts is developed and coatings with high quality, homogeneity and 30,000 inserting amount are secured. Coated parts are minimum 100 % more durable than non-coated parts.

Conditions for tumor-free and dopamine neuron-enriched grafts after transplanting human ES cell-derived neural precursor cells.

Ko, Ji-Yun,Lee, Hyun-Seob,Park, Chang-Hwan,Koh, Hyun-Chul,Lee, Yong-Sung,Lee, Sang-Hun Academic Press 2009 MOLECULAR THERAPY Vol.17 No.10

<P>We have previously demonstrated derivation of neural precursor (NP) cells of a midbrain-type from human embryonic stem (hES) cells to yield an enriched population of dopamine (DA) neurons. These hES-derived NPs can be expanded in vitro through multiple passages without altering their DA neurogenic potential. Here, we studied two aspects of these hES-NP cells that are critical issues in cell therapeutic approaches for Parkinson's disease (PD): cell survival and tumorigenic potential. Neuroepithelial rosettes, a potentially tumorigenic structure, disappeared during hES-NP cell expansion in vitro. Although a minor population of cells positive for Oct3/4, a marker specific for undifferentiated hES cells, persisted in culture during hES-NP cell expansion, they could be completely eliminated by subculturing hES-NPs under differentiation-inducing conditions. Consistently, no tumors/teratomas are formed in rats grafted with multipassaged hES-NPs. However, extensively expanded hES-NP cells easily underwent cell death during differentiation in vitro and after transplantation in vivo. Transgenic expression of Bcl-XL and sonic hedgehog (SHH) completely overcame the cell survival problems without increasing tumor formation. These findings indicate that hES-NP cell expansion in conjunction with Bcl-XL+SHH transgene expression may provide a renewable and safe source of DA neurons for transplantation in PD.</P>

Structural, electrical and optical properties of Al-doped ZnO thin films by pulsed DC magnetron sputtering

Ko, Hyung-Duk,Lee, Choong-Sun,Kim, Ki-Chul,Lee, Jae-Seok,Tai, Weon-Pil,Suh, Su-Jeong,Kim, Young-Sung The Korea Association of Crystal Growth 2004 한국결정성장학회지 Vol.14 No.4

We have investigated the structural, electrical and optical properties of Al-doped ZnO (AZO) thin films grown on glass substrate by pulsed DC magnetron sputtering as functions of pulse frequency and substrate temperature. A highly c-axis oriented AZO thin film is grown in perpendicular to the substrate when pulse frequency of 30 kHz and substrate temperature of $400^{\circ}C$ was applied. Under this optimized growth condition, the resistivity of AZO thin films exhibited $7.40\times 10^{-4}\Omega \textrm{cm}$. This indicated that the decrease of film resistivity resulted from the improvement of film crystallinity. The optical transmittance spectra of the films showed a very high transmittance of 85∼90 % in the visible wavelength region and exhibited the absorption edge of about 350 nm. The results show the potential application for transparent conductivity oxide (TCO) thin films.

A Measurement System for 3D Hand-Drawn Gesture with a PHANToM^TM Device

Ko, Seong-Young,Bang, Won-Chul,Kim, Sang-Youn Korea Information Processing Society 2010 Journal of information processing systems Vol.6 No.3

This paper presents a measurement system for 3D hand-drawn gesture motion. Many pen-type input devices with Inertial Measurement Units (IMU) have been developed to estimate 3D hand-drawn gesture using the measured acceleration and/or the angular velocity of the device. The crucial procedure in developing these devices is to measure and to analyze their motion or trajectory. In order to verify the trajectory estimated by an IMU-based input device, it is necessary to compare the estimated trajectory to the real trajectory. For measuring the real trajectory of the pen-type device, a PHANToMTM haptic device is utilized because it allows us to measure the 3D motion of the object in real-time. Even though the PHANToMTM measures the position of the hand gesture well, poor initialization may produce a large amount of error. Therefore, this paper proposes a calibration method which can minimize measurement errors.

Construction and Characterization of Novel Expression Vectors for Genetic Adipose Tissue Ablation

Ko, Duck Sung,Choi, Woong Hwan,Kim, Chul Geun The Korean Society for Integrative Biology 1998 Korean journal of biological sciences Vol.2 No.2

Obesity, one of the most common metabolic diseases in industrial countries is characterized by an increase in the number or size of adipocytes. In an effort to create transgenic mouse models for the study of obesity we developed a novel technique in which adipose tissue can be ablated genetically at will, at any specific developmental stage and/or physiological condition, by the treatment of ganciclovir. We made a series of adipocytespecific expression vectors using minimal regulatory regions of brown adipocyte-specific uncoupling protein (UCP-1) gene and adipocyte-specific aP2 gene, and then analyzed their expression characteristics in cultured cell lines. When both constructs pUCP-LacZ and paP2-LacZ were transfected transiently into differentiating 3T3-L1 (pre-while adipocytes) and HIB-1B (pre-brown adipocytes) cell lines in vitro and then monitored by X-gal staining of cells, these regulatory regions were sufficient to show proper differentiation stage-specific expression in adipocvtes. To confirm that adipocytes expressing HSV-TK controlled by these minimal requlatory elements are sufficient to kill themselves with ganciclovir treatment pUCP-TK and paP2-TK expression constructs were transfected stably into HIB-1B and 3T3-L1 cells, respectively, and their ganciclovir sensitivities were tested during in vitro differentiation of cells. As expected more than 80% of cells were dead by the 7th day of treatment with ganciclovir while negative control cells were not affected at all. The data suqqest that the constructed vectors are suitable for obtaining novel obese transqenic models based on a conditional genetic tissue ablation method.

Subcloning and Sequencing of β-Xylosidase Gene of Alkalophilic Bacillus sp. K17

SUNG, NACK KIE,SHIM, KI HWAN,CHUN, HYO GON,CHUNG, CHUNG HEE,CHUNG, YOUNG CHUL,KO, HAK RYONG 경상대학교 유전공학연구소 1988 遺傳工學硏究所報 Vol.7 No.-

The 5.0kb insert of pAX278, which was previously cloned, was reduced by various deletion and thus I. 4kb EcoRI-XbaI fragment was recloned into pUC19(pAK208). To increase β-xylosidase activity, the I. 4kb insert in pAK208 was recloned into expression vector, pKK223-3 (tac-promoter), and β-xylosidase activity in cell carring this plasmid (pKHR212) was increased was 3.5 times than that in pAX278. The complete nucleotide sequence of thermophilic alkalopilic Bacillus sp. K17 and its flanking regions were established. A 1263-bp open reading frame for β-xylosidase was observed. The molecular weight(50, 521 dalton) deduced from for β-xylosidase gene, was agreed with the result obtained by SDS-polyacry-lamide gel electrophoresis of the purified enzyme. The Shine-Dalgarno sequence was found-8 bp upstream of the initiation codon ATG. The 1-10 regions for promotor sequence of β-xylosidase gene were similar to the consensus sequence for B. subtilis RNA polymerase, and the-35 regions were different from all the known promotor for B. subtilis RNA polymerase.

On-farm Research Demonstration on Forage Production and Utilization in Yeongam, Yeonggwang, and Jangheung District, Korea

Sung Seo,Seong-Il Kim,Won-Ho Kim,Ki-Yong Kim,Sang-Hoon Lee,Jong-Geun Kim,Joung-Kyong Lee,Ji-Eon Park,Byung-Chul Lee,Hea-Shin Je,Chang-Ho Ko,Hong-Woog Kim 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

Nowadays, importance of regional specific on-farm research is largely increased in order to reduce the feeding cost of cattle. For this project, three regional districts (Yeongam, Yeonggwang and Jangheung) were selected wherein the cultivation area of forage production were largely increased from 1,200 ('08) to 2,600 ㏊ ('09) in Yeongam, 1,001('08) to 1,925 ㏊ ('09) in Yeonggwang, and 1,300 ('08) to 2,820 ㏊ ('09) in Jangheung district. Among the forage species, Italian ryegrass (IRG) and forage barley were predominantly cultivated in these regions. On the other hand, rye, grassland and summer annual forages were cultivated very little in a manner of land area. The productivity of forage barley was slightly increased 21.5('06), 26.2('07), and 27.3 MT/㏊ ('08) as silage. The number of farm and cultivation area was also increased remarkably. The present research project will be conducted from 2009 to 2010. Forage production and utilization for reduction of feeding cost, and high quality of Hanwoo beef production will investigated, in a manner of cultivation of forage crops mainly IRG, forage barley, IRG+barley mixture and corn.

The Effect of HPMC Concentration on the Morphology and Post Drawing of Wet Spun Regenerated SF/HPMC Blend Filaments

Ko, Jae-Sang,Um, In-Chul Korean Society of Sericultural Science 2009 International Journal of Industrial Entomology Vol.19 No.1

In this paper, the regenerated silk fibroin (SF)/hydroxypropyl methylcellulose (HPMC) blend filaments were prepared by wet spinning and the effect of HPMC concentration on the post drawing and morphology of blend filaments was elucidated. The result of maximum draw ratio indicated that the wet spinnability of wet spun SF / HPMC was improved with increasing HPMC concentration until 8% and remained constant after that concentration. The SEM observation revealed that the enhanced wet spinnability of blend filaments was strongly related to the morphological change by increasing HPMC concentration. Regardless of HPMC concentration, as SF content was reduced, the wet spinnability of blend film decreased resulting in reduced maximum draw ratio. It was also found by SEM observation that the cross section of blend filament deviated from circularity with an increase of HPMC content.