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- 저자 : San Gen Wang (검색결과 2 건)
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Inhibitory effects of piceatannol on human cytomegalovirus (hCMV) in vitro
Wang San-Ying,Zhang Jing,Xu Xiao-Gang,Su Hui-Li,Xing Wen-Min,Zhang Zhong-Shan,Jin Wei-Hua,Dai Ji-Huan,Wang Ya-Zhen,He Xin-Yue,Sun Chuan,Yan Jing,Mao Gen-Xiang 한국미생물학회 2020 The journal of microbiology Vol.58 No.8
Human cytomegalovirus (hCMV) is a ubiquitous herpesvirus, which results in the establishment of a latent infection that persists throughout the life of the host and can be reactivated when the immunity is low. Currently, there is no vaccine for hCMV infection, and the licensed antiviral drugs mainly target the viral enzymes and have obvious adverse reactions. Thus, it is important to search for compounds with antihCMV properties. The present study aimed to investigate the suppressive effects of piceatannol on hCMV Towne strain infection and the putative underlying mechanisms using human diploid fibroblast WI-38 cells. Piceatannol supplementation prevented the lytic changes induced by hCMV infection in WI-38 cells. Furthermore, piceatannol suppressed the expression of hCMV immediate-early (IE) and early (E) proteins as well as the replication of hCMV DNA in a dose-dependent manner. Moreover, hCMV-induced cellular senescence was suppressed by piceatannol, as shown by a decline in the senescence-associated β-galactosidase (SA-β-Gal) activity and decreased production of intracellular reactive oxygen species (ROS). p16INK4a, a major senescence-associated molecule, was dramatically elevated by current hCMV infection that was attenuated by pre-incubation with piceatannol in a dose-dependent manner. These results demonstrated that piceatannol suppressed the hCMV infection via inhibition of the activation of p16INK4a and cellular senescence induced by hCMV. Together, these findings indicate piceatannol as a novel and potent anti-hCMV agent with the potential to be developed as an effective treatment for chronic hCMV infection.
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Jun Lu,Jia Na Li,Bo Lei,San Gen Wang,You Rong Chai 한국유전학회 2009 Genes & Genomics Vol.31 No.2
Encoding a WD40 protein, Arabidopsis thaliana TRANSPARENT TESTA GLABRA1 (AtTTG1) regulates trichome and root hair differentiation as well as flavonoids and seed mucilage deposition in plants. Here, two Brassica napus TTG1 (BnTTG1) genes were isolated, and Southern hybridization also generated only two bands. The 1511-bp BnTTG1-1 and the 1555-bp BnTTG1-2 both have one intron, and show alternative sites for transcription start, polyadenylation and intron right border splicing. EST and GSS tags suggested that BnTTG1-1 was derived from B. rapa, while BnTTG1-2 from B. oleracea. Evidence implies that TTG1 was possibly triplicated in Brassiceae, but some triplicated members were lost soon, which might involve fragmental rearrangements. BnTTG1-1 shares 88.7% genomic and 95.7% mRNA identities with BnTTG1-2. Deduced BnTTG1-1 and BnTTG1-2 proteins both are 337 aa, differed only by substitution of a similar residue. They resemble AtTTG1 in WD40 domain and all conserved motifs. TTG1/AN11-type WD40 proteins are extremely conserved even across kingdoms. Homological and structural characterizations identified BnTTG1-1 and BnTTG1-2 to be orthologs of AtTTG1. Several non-coding motifs are conserved between AtTTG1 and BnTTG1. BnTTG1 coding regions tend to evolve high GC contents through T/A→C/G substitutions especially T→C transition, but AtTTG1 shows opposite base preference. BnTTG1 genes also evolve a GA-stretch in the leader sequence. RT-PCR detected complementation in organ-specificity between BnTTG1-1 and BnTTG1-2. BnTTG1-2 is more like AtTTG1 and is expressed in all major organs. BnTTG1-1 is more organ-specific with lower expression in seed and root, possibly withdrawing from regulating seed coat pigment/mucilage deposition and root hair formation.
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