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Aboelenain, Mansour,Balboula, Ahmed Zaky,Kawahara, Manabu,El-Monem Montaser, Abd,Zaabel, Samy Moawad,Kim, Sung-Woo,Nagano, Masashi,Takahashi, Masashi Elsevier 2017 Theriogenology Vol.91 No.-
<P><B>Abstract</B></P> <P>Recently, inhibition of cathepsin B (CTSB) activity during <I>in vitro</I> maturation (IVM) and culture (IVC) improved the developmental competence and quality of bovine oocytes and embryos. E-64 is a widely used inhibitor to inhibit CTSB activity, however, E-64 inhibits not only CTSB activity but also the activities of other proteases including cathepsin L (CTSL), papain, calpain, and trypsin. Pyridoxine, the catalytically active form of vitamin B6, plays a crucial role in several cellular processes and has the ability to inhibit CTSB activity. However, whether pyridoxine has an improving effect during IVM of bovine oocytes is still unknown. In this study, we investigated the effect of pyridoxine supplementation during IVM on the developmental competence of bovine oocytes and the quality of the produced blastocysts. Supplementation of pyridoxine to the maturation medium significantly decreased the activity of CTSB in both bovine cumulus cells and oocytes. Moreover, pyridoxine improved both the blastocyst and hatched blastocyst rates. In addition, the presence of pyridoxine during IVM also significantly improved the quality of the produced embryos by increasing the total cell number as well as decreasing the CTSB mRNA expression and apoptotic rate. These results indicate that pyridoxine is a promising tool to improve the developmental competence of bovine oocytes and subsequent embryo quality.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We investigated the effect of pyridoxine during IVM on the developmental competence of bovine oocytes. </LI> <LI> Supplementation of pyridoxine during IVM significantly decreased the activity of CTSB in bovine COCs. </LI> <LI> Pyridoxine improved the developmental competence of bovine oocytes by increasing the blastocyst rate. </LI> <LI> Pyridoxine improved the quality of the produced embryos by increasing the TCN and decreasing the apoptotic rate. </LI> <LI> Therefore, pyridoxine is a promising tool to improve the developmental competence of bovine oocytes and embryo quality. </LI> </UL> </P>
Asmaa Bakr,Kazuo Hara,Moaz Elshair,Shin Haba,Takamichi Kuwahara,Nozomi Okuno,Daiki Fumihara,Takafumi Yanaidani,Samy Zaky,Hanaa Omar 대한소화기내시경학회 2023 Clinical Endoscopy Vol.56 No.3
Background/Aims: Endoscopic ultrasound (EUS)-guided fine-needle aspiration (FNA) using oblique-view EUS in patients with a surgically altered anatomy (SAA) of the upper gastrointestinal tract is limited because of difficult scope insertion due to the disturbed anatomy. This study aimed to investigate the efficiency of forward-view (FV)-EUS in performing FNA in patients with a SAA. Methods: We retrospectively investigated 32 patients with a SAA of the upper gastrointestinal tract who visited Aichi Cancer Center Hospital in Nagoya, Japan, between January 2014 and December 2020. We performed upper gastrointestinal EUS-FNA using FV-EUS combined with fluoroscopic imaging to confirm tumor recurrence or to make a decision before chemotherapy or after a failure of diagnosis by radiology. Results: We successfully performed EUS-FNA in all studied patients (100% technical success), with the specificity, sensitivity, and accuracy of 100%, 87.5%, and 87.8%, respectively, with no complications. Conclusions: EUS-FNA using FV-EUS combined with fluoroscopic imaging is an effective and safe technique for tissue acquisition in patients with a SAA.