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        Characterization and Comparative Evaluation of Milk Protein Variants from Pakistani Dairy Breeds

        Iqra Yasmin,Rabia Iqbal,Atif Liaqat,Wahab Ali Khan,Muhamad Nadeem,Aamir Iqbal,Muhammad Farhan Jahangir Chughtai,Syed Junaid Ur Rehman,Saima Tehseen,Tariq Mehmood,Samreen Ahsan,Saira Tanweer,Saima Naz 한국축산식품학회 2020 한국축산식품학회지 Vol.40 No.5

        The aim of study was to scrutinize the physicochemical and protein profile of milk obtained from local Pakistani breeds of milch animals such as Nilli-Ravi buffalo, Sahiwal cow, Kajli sheep, Beetal goat and Brela camel. Physicochemical analysis unveiled maximum number of total solids and protein found in sheep and minimum in camel. Buffalo milk contains the highest level of fat (7.45%) while camel milk contains minimum (1.94%). Ash was found maximum in buffalo (0.81%) and sheep (0.80%) while minimum in cow’s milk (0.71%). Casein and whey proteins were separated by subjecting milk to isoelectric pH and then analyzed through sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The results showed heterogeneity among these species. Different fractions including αS1, αS2, κ-casein, β-casein and β-lactoglobulen (β-Lg) were identified and quantitatively compared in all milk samples. Additionally, this electrophoretic method after examining the number and strength of different protein bands (αS1, αS2, β- CN, α-LAC, BSA, and β-Lg, etc.), was helpful to understand the properties of milk for different processing purposes and could be successfully applied in dairy industry. Results revealed that camel milk was best suitable for producing allergen free milk protein products. Furthermore, based on the variability of milk proteins, it is suggested to clarify the phylogenetic relationships between different cattle breeds and to gather the necessary data to preserve the genetic fund and biodiversity of the local breeds. Thus, the study of milk protein from different breed and species has a wide range of scope in producing diverse protein based dairy products like cheese.

      • KCI우수등재

        Prevalence and Sociodemographic Factors of Overweight and Obesity among Pakistani Adults

        Muhammad Asif,Muhammad Aslam,Saima Altaf,Saima Atif,Abdul Majid 대한비만학회 2020 The Korean journal of obesity Vol.29 No.1

        Background: Obesity is a serious public health problem that is growing alarmingly worldwide. The main objective of this study is to examine the current prevalence of overweight and obesity among Pakistani adults and to identify the sociodemographic factors that are associated with overweight and obesity. Methods: Secondary data from a population-based household survey (the Pakistan Panel Household Survey) were used for this study. A total of 10,063 participants (3,916 men and 6,147 women) were included. Overweight and obese individuals were identified using the World Health Organization-recommended cutoffs for body mass index (kg/m2). Along with descriptive statistics, the Pearson chi-square test was used to investigate the association among categorical variables. Odds ratios (ORs) along with 95% confidence intervals (CIs) were estimated using univariate logistic regression analysis to evaluate the most significantly associated risk factors for overweight/obesity. Results: The mean age and body mass index of the participants were 38.2 years and 22.9 kg/m2, respectively. Overall, 22.8% of the participants (23.9% of the women and 21.1% of the men) were overweight, and 5.1% (6.3% of the women and 3.2% of the men) were obese. Sociodemographic factors, such as sex, marital status, and residential area, were significantly associated with body mass index categories. Women (OR, 1.34; 95% CI, 1.23–1.47; P<0.01), ever-married individuals (OR, 1.92; 95% CI, 1.70–2.16; P<0.01), and individuals living in urban areas (OR, 1.23; 95% CI, 1.12–1.35; P<0.01) were more likely to become overweight/obese than their counterparts. Conclusion: We have found the excess weight problem to be quite high in the Pakistani adult population. The government and other health agencies should take initiatives in launching programs about nutritional awareness for adults to prevent obesity.

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