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M. K. Ghosh,B. K. Das,C. Das,A. K. Mishra,P. K. Mukherjee,S. Raje Urs 한국잠사학회 2004 International Journal of Industrial Entomology Vol.8 No.1
Ten improved mulberry varieties (V1, C1730, C2016, C2017, Anantha, RFS-175, Thallaghatapura, Vishala, S1 and S1635) were evaluated through enzyme assay and estimation of soluble protein content followed by regression analysis, grown under irrigated conditions in the alluvial soils of Gangetic plains of West Bengal in India for five successive crops in a year. The nitrate reductase (EC No. 1.6.6.1) activity (NRA, mmol NO2 - h-1g-1 fr. wt.), total soluble protein (mg g-1 fr. wt.) was estimated which showed to vary significantly in the tested varieties. In addition to these, the other parameters like unit leaf fresh and dry weight (g), moisture%, unit leaf area (cm2), specific leaf weight (g cm-2), total soluble sugar (mg g-1 fr. wt.), leaf yield/plant (kg), shoot yield/plant (kg) and net photosynthetic rate (NPR, mmol m-2 s-1) were also studied which showed to vary significantly in tested varieties. Among them, S1635, having higher NRA (13.25 mmol NO2 - h-1g-1 fr. wt.), total soluble protein (39.63 mg g-1 fr. wt.), NPR (16.66 mmol m-2 s-1), total soluble sugar (48.44 mg g-1 fr. wt.), leaf yield/plant (0.689 kg) and shoot yield/plant (1.135 kg) showed its superiority over other tested varieties. Regression and correlation coefficients were analysed, and a strong positive correlation was found between NRA & total soluble protein, NRA & NPR, NRA & total soluble sugar, NRA & unit leaf weight, NRA & specific leaf weight, NRA & leaf yield/plant, NRA & shoot yield/plant, NPR & leaf yield and NPR & specific leaf weight.
( M. K. Ghosh ),( B. K. Das ),( C. Das ),( A. K. Mishra ),( P. K. Mukherjee ),( S. Raje Urs ) 한국잠사학회 2007 International Journal of Industrial Entomology Vol.8 No.1
Ten improved mulberry varieties (Vl, C1730, C2016, C2017, Anantha, RFS-175, Thallaghatapura, Vishala, S1 and S1635) were evaluated through enzyme assay and estimation of soluble protein content followed by regression analysis, grown under irrigated conditions in the alluvial soils of Gangetic plains of West Bengal in India for five successive crops in a year. The nitrate reductase (EC No. 1.6.6.1) activity (NRA, μmol NO2-h-1 g-1 fr, wt.), total soluble protein (mg g-1 fr, wt.) was estimated which showed to vary significantly in the tested varieties. In addition to these, the other parameters like unit leaf fresh and dry weight (g), moisture %, unit leaf area (㎠), specific leaf weight (g ㎝-2), total soluble sugar (mg g-1 fr, wt.), leaf yield/plant (kg), shoot yield/plant (kg) and net photosynthetic rate (NPR, μ㏖ m-2 g-1) were also studied which showed to vary significantly in tested varieties. Among them, S1635, haying higher NRA (13.25 μ㏖ NO2-h-1g-1 fr, wt.), total soluble protein (39.63 mg g-1 fr, wt.), NPR (16.66 μ㏖ m-2 S-1), total soluble sugar (48.44 mg g-1 fr. wt.), leaf yield/plant (0.689 kg) and shoot yield/plant (1.135 kg) showed its superiority over other tested varieties. Regression and correlation coefficients were analysed, and a strong positive correlation was found between NRA & total soluble protein, NRA & NPR, NRA & total soluble sugar, NRA & unit leaf weight, NRA & specific leaf weight, NRA & leaf yield/plant, NRA & shoot yield/plant, NPR & leaf yield and NPR & specific leaf weight.
Ghosh, M.K.,Das, B.K.,Das, C.,Mishra, A.K.,Mukherjee, P.K.,Urs, S.Raje Korean Society of Sericultural Science 2004 International Journal of Industrial Entomology Vol.8 No.1
Ten improved mulberry varieties (Vl, C1730, C2016, C2017, Anantha, RFS-175, Thallaghatapura, Vishala, S1 and S1635) were evaluated through enzyme assay and estimation of soluble protein content followed by regression analysis, grown under irrigated conditions in the alluvial soils of Gangetic plains of West Bengal in India for five successive crops in a year, The nitrate reductase (EC No. 1.6.6.1) activity (NRA, $\mu$mol N $O_2$- $h^{-1}$ $g^{-1}$ fr, wt.), total soluble protein (mg $g^{-1}$ fr, wt.) was estimated which showed to vary significantly in the tested varieties. In addition to these, the other parameters like unit leaf fresh and dry weight (g), moisture %, unit leaf area ($\textrm{cm}^2$), specific leaf weight (g c $m^{-2}$ ), total soluble sugar (mg $g^{-1}$ fr, wt.), leaf yield/plant (kg), shoot yield/plant (kg) and net photosynthetic rate (NPR, $\mu$㏖ $m^{2}$ $s^{-1}$ ) were also studied which showed to vary significantly in tested varieties. Among them, S1635, haying higher NRA (13.25 $\mu$㏖ N $O_2$- $h^{-l}$ $g^{-1}$ fr, wt.), total soluble protein (39.63mg $g^{-1}$ fr, wt.), NPR(16.66 $\mu$㏖ $m^{-2}$ $s^{-1}$ ), total soluble sugar (48.44 mg $g^{-1}$ fr. wt.), leaf yield/plant (0.689 kg) and shoot yield/plant (1.135 kg) showed its superiority over other tested varieties. Regression and correlation coefficients were analysed, and a strong positive correlation was found between NRA & total soluble protein, NRA & NPR, NRA & total soluble sugar, NRA af unit leaf weight, NRA & specific leaf weight, NRA & leaf yield/plant, NRA & shoot yield/plant, NPR & leaf yield and NPR & specific leaf weight.t.
Yoon, S.H.,Lee, S.H.,Das, A.,Ryu, H.K.,Jang, H.J.,Kim, J.Y.,Oh, D.K.,Keasling, J.D.,Kim, S.W. Elsevier Science Publishers 2009 Journal of biotechnology Vol.140 No.3
The increased synthesis of building blocks of IPP (isopentenyl diphosphate) and DMAPP (dimethylallyl diphosphate) through metabolic engineering is a way to enhance the production of carotenoids. Using E. coli as a host, IPP and DMAPP supply can be increased significantly through the introduction of foreign MVA (mevalonate) pathway into it. The MVA pathway is split into two parts with the top and bottom portions supplying mevalonate from acetyl-CoA, and IPP and DMAPP from mevalonate, respectively. The bottom portions of MVA pathway from Streptococcus pneumonia, Enterococcus faecalis, Staphylococcus aureus, Streptococcus pyogenes and Saccharomyces cerevisiae were compared with exogenous mevalonate supplementation for β-carotene production in recombinant Escherichia coli harboring β-carotene synthesis genes. The E. coli harboring the bottom MVA pathway of S. pneumoniae produced the highest amount of β-carotene. The top portions of MVA pathway were also compared and the top MVA pathway of E. faecalis was found out to be the most efficient for mevalonate production in E. coli. The whole MVA pathway was constructed by combining the bottom and top portions of MVA pathway of S. pneumoniae and E. faecalis, respectively. The recombinant E. coli harboring the whole MVA pathway and β-carotene synthesis genes produced high amount of β-carotene even without exogenous mevalonate supplementation. When comparing various E. coli strains - MG1655, DH5α, S17-1, XL1-Blue and BL21 - the DH5α was found to be the best β-carotene producer. Using glycerol as the carbon source for β-carotene production was found to be superior to glucose, galactose, xylose and maltose. The recombinant E. coli DH5α harboring the whole MVA pathway and β-carotene synthesis genes produced β-carotene of 465mg/L at glycerol concentration of 2% (w/v).
S. ROY,A. K. DAS,R. BANERJEE 한국자동차공학회 2016 International journal of automotive technology Vol.17 No.1
The present study attempts to address the challenges of the multiobjective optimization problem of the BSFCNOx- PM trade-off paradox of an existing diesel engine by harnessing the synergetic benefit of PM and BSFC reduction through CRDI operation and simultaneous NOx reduction by EGR application. Load, FIP and EGR were chosen as the input parameters while NOx, PM and BSFC were the response variables. In order to reduce the experimental effort, the Taguchi L16 orthogonal array technique was employed to obtain the corresponding values of the response variables. The grey relational analysis coupled with fuzzy logic has been employed as the optimization routine. The optimal combination of the input parameters corresponding to the calibrated values of the response variables were obtained by employing the Grey-Fuzzy Grade and S-N ratio strategy as a performance index. The computed optimal combination so obtained were further validated through actual experimentation. EGR was found to be the most influencing factor in the present optimization endeavour. The study also established that the Grey-Fuzzy-Taguchi method was not only comparable but superior to the Grey-Taguchi method usually employed for such optimization studies.
( S. M. Moorthy ),( S. K. Das ),( P. R. T. Rao ),( S. Raje Urs ),( A. Sarkar ) 한국잠사학회 2007 International Journal of Industrial Entomology Vol.14 No.1
In order to find out the appropriate parents for the breeding programme, twelve bivoltine and three multivoltine silkworm breeds were evaluated on the basis of multivariate selection index and isozyme analysis. Of which, four [CSR2, D6 (P), SK3, SK4] bivoltine and two multivoltine (Nistari, Cambodge) breeds were selected and breeding initiated to develop higher survival bivoltine silkworm breed suitable for tropical conditions. Among two isozyme (Esterase and acid phosphatase) analyzed, only esterase exhibited polymorphism among the bivoltine breeds. No polymorphism was observed among multivoltine in respect of esterase as well as acid phosphatase.
K. Sandeep,R. S. Negi,A. S. Panicker,Alok Sagar Gautam,D. S. Bhist,G. Beig,B. S. Murthy,R. Latha,Santosh Singh,S. Das 한국기상학회 2020 Asia-Pacific Journal of Atmospheric Sciences Vol.56 No.3
Extraction of organic carbon (OC) and elemental carbon (EC) were carried out over Srinagar, India, an ecologically sensitive semi-urban site in Garhwal Himalays. The PM2.5 sampling was carried out during January to December, 2017 over the site. The OC and EC were extracted from PM2.5 samples using a thermo optical OC/EC analyzer. Highest OC and EC concncentrations were found during postmonsoon (17.67 ± 1.1 μg/m3 OC and 6.34 ± 0.75 EC) and Winter (17.31 ± 3.045 μg/m3 OC and 6.32 ± 0.585 μg/m3 EC) seasons are attributed to boundary layer dynamics and anthropogenic activities. The lower concentration of OC/ EC was observed during monsoon season (11.64 ± 1.75 μgm−3 OC and 3.46 ± 0.19μgm−3 EC) owing to wet scavenging of aerosols and minimum count of forest fire/biomass buring incidences. Both pre-monsoon and post-monsoon season concentrations are also influenced by biomass burning in the IGP (Indo-Gangetic Plain) region and forest fires in the adjecent areas. The OC/EC ratio sounds that vehicular exhaust and biomass burning are the major source of OC/EC over the site. Generation of secondary organic carbon (SOC) at the region causes variability in OC/EC ratio in different seasons. It is found that 24–32% of PM2.5 is contributed by carbonaceous aerosols (OC and EC) over Srinagar. The pivotal role of meteorology in modulating OC/ EC concentrations has been illustared in detail.