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RISS 인기검색어
- 검색키워드
- 저자 : Ryulyi Kang (검색결과 2 건)
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Radio Sensitivity of Cowpea Plants after Gamma-Ray and Proton-Beam Irradiation
Ryulyi Kang,EunJuSeo,김규태,박아론,김운지,강시용,하보근 한국육종학회 2020 Plant Breeding and Biotechnology Vol.8 No.3
Cowpea (Vigna unguiculata (L.) Walp.) is one of the important legume crops in which all stages of plant growth are used in human food and animal feed. In this study, morphological and biochemical changes were investigated in cowpea plants exposed to proton-beams and gamma-rays. ‘Okdang’ cultivar seeds were irradiated with 100, 200, 300, 400, and 500 Gy of gamma-rays and proton-beams. The gamma-ray and proton-beam irradiations significantly delayed emergence period, but had small effects on emergence rates. Compared to a control, the proton-beam treatments showed a significant decrease in survival rate above 200 Gy. The gamma-ray treatments significantly reduced survival rate above 300 Gy. The Lethal Dose 50 (LD50) for both irradiations were 200-300 Gy. Plant height and fresh shoot weight tended to decrease with increasing radiation levels. The Reduction Dose 50 (RD50) of plant height was between 100 and 200 Gy for the proton-beam and 200 to 300 Gy for the gamma-ray treatments. The RD50 of fresh shoot weight was expected to be 500 Gy or higher for the proton-beam and gamma-ray treatments. The malonaldehyde (MDA) content in both treatments were slightly decreased at 200 Gy compared to a control, but increased at 300 Gy. Overall, antioxidant enzymes reacted more to proton-beams than to gamma-rays. However, gamma-rays appear to have a greater effect on chlorophyll content than protonbeams. Our research will be valuable as a basic research comparing the mutagenic effects on cowpea induced by two ionizing radiations.
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Genome-Wide Association Study for Flowering Time in Korean Cowpea Germplasm
서은주,김기풍,Ryulyi Kang,김규태,박아론,김운지,선호근,하보근 한국육종학회 2020 Plant Breeding and Biotechnology Vol.8 No.4
Cowpea is an annual legume crop; although it is an essential food in developing countries, cowpea is now grownworldwide. For the genetic improvement of plants, flowering time is one of the major selection criteria. In general, flowering isregulated by photoperiod and temperature, along with the interaction between environmental factors. In this study, we aimed toinvestigate the candidate genes associated with flowering time using genome-wide association study (GWAS). To investigate theflowering time-related genes, 384 cowpea germplasms were genotyped with 51,128 single nucleotide polymorphisms (SNPs). Themain genetic component of days to flowering (DTF) was analyzed using genome association and prediction integrated tool (GAPIT)and elastic-net analyses. From the GAPIT and elastic-net analyses, a total of 23 SNPs were significantly associated with DTF amongfive (chr. 2, 3, 7, 9, and 11) and seven different chromosomes (chr. 1, 2, 3, 4, 5, 8, and 9), respectively. Based on our analysis,Vigun01g084000, Vigun01g227200, Vigun02g062600, and Vigun03g296800 were considered the major candidate genes that weresignificantly associated with DTF in cowpea. These results confirmed that DTF might be controlled by multiple genes affecting earlyflowering, delaying flowering time, repressing the transition to flowering, etc. This study will potentially contribute to effective DTFgenomic selection in plant breeding to better understand the genetic basis and explore the mechanism of flowering time.
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