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- 저자 : Rohman Motiar (검색결과 3 건)
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Masayuki Fujita,Md Motiar Rohman,Md Daud Hossain 한국작물학회 2007 Journal of crop science and biotechnology Vol.10 No.1
Glutathione S-transferases (GSTs, EC 2.5.1.18), glyoxalase-I (EC 4.4.1.5) and alliin lyase (alliinase, EC 4.4.1.4) are important enzyme systems in plant bodies. The first two are mainly detoxifying enzymes that utilize glutathione (GSH) in the defense mechanism, and the last one is mainly involved in secondary metabolism and relevant to sulfur compounds derived from GSH. The activities of the three enzymes have been investigated in soluble extracts of vegetable crops, including pumpkin, cabbage, broccoli, radish, carrot, potato, sweet potato, mungbean, and onion. GST activities were detected in all of the vegetables, and the extract of onion bulb exhibited the highest specific activity (648 nmol/min/mgP). The putative GSTs of most of the vegetables were found to be induced by ethanol. The activities of GSTs in onion bulb were found to be markedly inhibited by S-hexyl glutathione and were also inhibited by S-butyl glutathione and S-propyl glutathione. The anti- CmGSTF1 antiserum recognized a thick band for putative onion GST. The estimated glyoxalase-I activity level was also high in onion bulb (4540 nmol/min/mgP), indicating that the thick band detected by Western blot analysis might result from partial recognition of glyoxalase-I by the antiserum. The specific activities for glyoxalase-I were moderate in radish and carrot, and the extracts of other vegetables had rather low levels of activities. The extract of onion also showed the highest specific activity level for alliinase (2069nmol pyruvate/mgP). The extracts of other vegetables also had alliinase activities, although the estimated values were much lower than that of onion. Glutathione S-transferases (GSTs, EC 2.5.1.18), glyoxalase-I (EC 4.4.1.5) and alliin lyase (alliinase, EC 4.4.1.4) are important enzyme systems in plant bodies. The first two are mainly detoxifying enzymes that utilize glutathione (GSH) in the defense mechanism, and the last one is mainly involved in secondary metabolism and relevant to sulfur compounds derived from GSH. The activities of the three enzymes have been investigated in soluble extracts of vegetable crops, including pumpkin, cabbage, broccoli, radish, carrot, potato, sweet potato, mungbean, and onion. GST activities were detected in all of the vegetables, and the extract of onion bulb exhibited the highest specific activity (648 nmol/min/mgP). The putative GSTs of most of the vegetables were found to be induced by ethanol. The activities of GSTs in onion bulb were found to be markedly inhibited by S-hexyl glutathione and were also inhibited by S-butyl glutathione and S-propyl glutathione. The anti- CmGSTF1 antiserum recognized a thick band for putative onion GST. The estimated glyoxalase-I activity level was also high in onion bulb (4540 nmol/min/mgP), indicating that the thick band detected by Western blot analysis might result from partial recognition of glyoxalase-I by the antiserum. The specific activities for glyoxalase-I were moderate in radish and carrot, and the extracts of other vegetables had rather low levels of activities. The extract of onion also showed the highest specific activity level for alliinase (2069nmol pyruvate/mgP). The extracts of other vegetables also had alliinase activities, although the estimated values were much lower than that of onion.
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Hossain, Md Daud,Rohman, Md Motiar,Fujita, Masayuki The Korean Society of Crop Science 2007 Journal of crop science and biotechnology Vol.10 No.1
Glutathione S-transferases(GSTs, EC 2.5.1.18), glyoxalase-I(EC 4.4.1.5) and alliin lyase(alliinase, EC 4.4.1.4) are important enzyme systems in plant bodies. The first two are mainly detoxifying enzymes that utilize glutathione(GSH) in the defense mechanism, and the last one is mainly involved in secondary metabolism and relevant to sulfur compounds derived from GSH. The activities of the three enzymes have been investigated in soluble extracts of vegetable crops, including pumpkin, cabbage, broccoli, radish, carrot, potato, sweet potato, mungbean, and onion. GST activities were detected in all of the vegetables, and the extract of onion bulb exhibited the highest specific activity(648 nmol/min/mgP). The putative GSTs of most of the vegetables were found to be induced by ethanol. The activities of GSTs in onion bulb were found to be markedly inhibited by S-hexyl glutathione and were also inhibited by S-butyl glutathione and S-propyl glutathione. The anti-CmGSTF1 antiserum recognized a thick band for putative onion GST. The estimated glyoxalase-I activity level was also high in onion bulb(4540 nmol/min/mgP), indicating that the thick band detected by Western blot analysis might result from partial recognition of glyoxalase-I by the antiserum. The specific activities for glyoxalase-I were moderate in radish and carrot, and the extracts of other vegetables had rather low levels of activities. The extract of onion also showed the highest specific activity level for alliinase(2069nmol pyruvate/mgP). The extracts of other vegetables also had alliinase activities, although the estimated values were much lower than that of onion.
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Mutant divergence in sunflower induced through gamma radiation
Habib Sheikh Hasna,Akanda Abdul Latif,Roy Pryanka,Kulsum Ummy,Rohman Motiar,Rahman M. M. 한국작물학회 2023 Journal of crop science and biotechnology Vol.26 No.3
Gamma radiation-induced mutation divergence was studied in sunfower variety BARI Surjamukhi-2. A number of treatment combinations from T1 to T5 (100 to 500 gamma doses) were applied at the laboratory of Institute of Food and Radiation Biology, Ganakbari, Savar, and M1 and M2 generations were grown at the research feld of Oilseed Research Centre (ORC), Bangladesh Agricultural Research Institute (BARI), Gazipur. Compared to the control plant, most of the mutagenic treatments in M1 generation showed a gradual reduction of mean performance. The seeds treated with 100 and 200 gamma doses produced taller plant than control, while seeds treated with 400 and 500 gamma doses did not survive or produce any seed to evaluate in further generation. The T3-treated (300 gamma dose) seeds produced plants of average height. Dwarfsm and semi dwarfsm being the most desirable traits in sunfower, the seeds obtained in T3 were grown to generate M2 population for further evaluation. In M2 generation, compared to control, plant improvement was recorded for plant stature (short and robust), increased head size, seed number, and seed yield. Besides quantitative divergence, qualitative divergence was also very obvious—such as diferent size, shape and orientation in head character, robust stem, broad leaf, short internode, tall mutants, branching mutants, diferent size, shape and seed coat color compared to non-treated seed. All these diverse mutants may have breeding value and be put into further research.
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