Effects of Topical Tamoxifen on Wound Healing of Burned Skin in Rats

Mehrvarz, Shaban,Ebrahimi, Ali,Sahraei, Hedayat,Bagheri, Mohammad Hasan,Fazili, Sima,Manoochehry, Shahram,Rasouli, Hamid Reza Korean Society of Plastic and Reconstructive Surge 2017 Archives of Plastic Surgery Vol.44 No.5

Background This study aimed to assess the effects of the topical application of tamoxifen on wound healing of burned skin in Wistar rats by evaluating 3 healing characteristics: fibrotic tissue thickness (FTT), scar surface area (SSA), and angiogenesis in the healed scar tissue. Methods Eighteen male Wistar rats were used in this study. A third-degree burn wound was made on the shaved animals' back, measuring $2{\times}2{\times}2cm$. In the first group, a 2% tamoxifen ointment was applied to the wound twice daily for 8 weeks. The second group received a placebo ointment during the same period. The third group did not receive any treatment and served as the control group. Results The median (interquartile range=[Q1, Q3]) FTT was 1.35 (1.15, 1.62) mm, 1.00 (0.95, 1.02) mm, and 1.25 (0.8, 1.5) mm in the control, tamoxifen, and placebo groups, respectively (P=0.069). However, the FTT in the tamoxifen group was less than in the placebo and control groups. The median angiogenesis was 3.5 (3.00, 6.25), 8.00 (6.75, 9.25), and 7.00 (5.50, 8.25) vessels per high-power field for the control, tamoxifen, and placebo groups, respectively (P=0.067). However, the median angiogenesis was higher in the tamoxifen group than in the control group. No significant difference was observed in the mean SSA between the tamoxifen group and the control group (P=0.990). Conclusions Local application of tamoxifen increased angiogenesis and decreased the FTT, with no change in the SSA in burned skin areas. These effects are expected to expedite the wound healing process, reducing contracture and preventing hypertrophic scar and keloid formation.

Application of experimental design for removal of sunset yellow by copper sulfide nanoparticles loaded on activated carbon

Saeid Khodadoust,Mehrorang Ghaedi,Reza Sahraei,Ali Daneshfar 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.5

In this study, copper sulfide nanoparticles loaded on activated carbon (CuS-NP-AC) was synthesized by novel, low cost and green approach and characterized using different techniques such as SEM, and BET. This material was used for the removal of sunset yellow (SY) from aqueous solutions was investigated. The dependency of removal percentages to variables such as pH, initial SY concentration, adsorbent dosage and sonication time were studied by central composite design (CCD) coupled with response surface methodology (RSM) by considering the desirability function (DF). The accuracy and ability of method at optimum values predicted by this model was studied by conduction of similar experiments at the same previously optimized conditions. A good agreement between experimental and predicted data was achieved that efficiency of this model for prediction of real optimum point. Among the well known previously isotherm models, the experimental equilibrium data efficiently can be represented by the Langmuir model, while the rate of adsorption. Kinetic data efficiently can be interpreted by combination of pseudo-second order as well as intraparticle diffusion models. The small amount of this adsorbent (0.016 g) is applicable for removal of high amount of SY (>90%) in reasonable time (17 min).

Comparison of the influence of nanomaterials on response properties of copper selective electrodes

Mehrorang Ghaedi,Morteza Montazerozohori,Reza Sahraei 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.4

Novel Cu2+ ion selective electrode in carbon paste matrices based on incorporation of bis(2-hydroxynaphthaldehydene)-1,6-hexanediamine (BHNHDA) has been developed. The influence of variables including sodium tetraphenylborate (NaTPB), ionophore, and amount of multiwalled carbon nanotubes (MWCNT) and Nujol and effect of two new nanoparticles including gold nanoparticles loaded on activated carbon (Au-NP-AC) and zinc sulfide nanoparticles loaded on activated carbon (ZnS-NP-AC)on the electrodes response was studied and optimized. At optimum specified conditions, the proposed electrodes have appropriate advantages such as short response times and suitable reproducibility and applicability for a period of at least 1 month without any significant divergence in slope and response properties. The sensor based on impregnations of MWCNT, Au-NP-AC and ZnS-NP-AC have wide linear range of concentration (6 x 10-8–1.0 x 10-1 mol L-1) and detection limit of lower than 4 x 10-8 mol L-1of Cu2+ ion. The electrodes based on incorporation of Au-NP-AC and ZnS-NP-AC have Nernstian response with slope of 29.34 ± 1.41 and 29.78 ± 1.23 mV decade-1 and response is independent of pH in the range of 2.0–5.0. Finally, these electrodes have been successfully applied for the determination of Cu2+ ions content in various real samples. Due to their acceptable selectivity coefficient, they are usable for accurate and successful evaluation of Cu2+ ions content in various real sample with complicated matrices.

Effects of Topical Tamoxifen on Wound Healing of Burned Skin in Rats

Shaban Mehrvarz,Ali Ebrahimi,Hedayat Sahraei,Mohammad Hasan Bagheri,Sima Fazili,Shahram Manoochehry,Hamid Reza Rasouli 대한성형외과학회 2017 Archives of Plastic Surgery Vol.44 No.5

Background This study aimed to assess the effects of the topical application of tamoxifen on wound healing of burned skin in Wistar rats by evaluating 3 healing characteristics: fibrotic tissue thickness (FTT), scar surface area (SSA), and angiogenesis in the healed scar tissue. Methods Eighteen male Wistar rats were used in this study. A third-degree burn wound was made on the shaved animals’ back, measuring 2×2×2 cm. In the first group, a 2% tamoxifen ointment was applied to the wound twice daily for 8 weeks. The second group received a placebo ointment during the same period. The third group did not receive any treatment and served as the control group. Results The median (interquartile range=[Q1, Q3]) FTT was 1.35 (1.15, 1.62) mm, 1.00 (0.95, 1.02) mm, and 1.25 (0.8, 1.5) mm in the control, tamoxifen, and placebo groups, respectively (P=0.069). However, the FTT in the tamoxifen group was less than in the placebo and control groups. The median angiogenesis was 3.5 (3.00, 6.25), 8.00 (6.75, 9.25), and 7.00 (5.50, 8.25) vessels per high-power field for the control, tamoxifen, and placebo groups, respectively (P=0.067). However, the median angiogenesis was higher in the tamoxifen group than in the control group. No significant difference was observed in the mean SSA between the tamoxifen group and the control group (P=0.990). Conclusions Local application of tamoxifen increased angiogenesis and decreased the FTT, with no change in the SSA in burned skin areas. These effects are expected to expedite the wound healing process, reducing contracture and preventing hypertrophic scar and keloid formation

Palladium, silver, and zinc oxide nanoparticles loaded on activated carbon as adsorbent for removal of bromophenol red from aqueous solution

Mehrorang Ghaedi,Maryam Ghayedi,Syamak Nasiri Kokhdan,Reza Sahraei,Ali Daneshfar 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.4

The adsorption of bromophenol red (BPR) onto three adsorbents including palladium, silver and zinc oxide nanoparticles loaded on activated carbon (Pd-NP-AC, Ag-NP-AC and ZnO-NP-AC) in a batch system has been studied and the influence of various parameters has been optimized. The influence of time on removal of BPR on all adsorbent was investigated and experimental data were analyzed by four kinetic models including pseudo first and second-order, Elovich and the intraparticle diffusion equations. Following fitting the experimental data to these models, the respective parameters of each model such as rate constants, equilibrium adsorption capacities and correlation coefficients for each model were investigated and based on well known criterion their applicability was judged. It was seen that the adsorption of BPR onto all adsorbents sufficiently described by the pseudo second-order equation in addition to interparticle diffusion model. The adsorption of BPR on all adsorbent was investigated at various concentration of dye and the experimental equilibrium data were analyzed and fitted to the Langmuir, Freundlich, Tempkin, Dubinin, and Radushkevich equations. A single stage in batch process was efficient and suitable for all adsorbents using the Langmuir isotherm with maximum adsorption of 143 mg gˉ1 for Pd-NP-AC, 250 mg gˉ1 for Ag-NP-AC and 200 mg gˉ1 for ZnO-NR-AC. Thermodynamic parameters such as DG8, DH8, and DS8 for Pd-NP-AC adsorbent were calculated.

Competitive adsorption of Direct Yellow 12 and Reactive Orange 12 on ZnS:Mn nanoparticles loaded on activated carbon as novel adsorbent

Shaaker Hajati,Mehrorang Ghaedi,Farahnaz Karimi,Behnaz Barazesh,Reza Sahraei,Ali Daneshfar 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.2

A novel adsorbent (ZnS:Mn nanoparticles loaded on activated carbon) was made. The competitiveadsorption of Direct Yellow 12 (DY12) and Reactive Orange 12 (RO12) dyes in binary mixture onto thisadsorbent was studied. DY12 and RO12 with severe spectra overlapping were chosen and analyzedsimultaneously with high accuracy by first order derivative spectrophotometric method in binarysolutions. The effect of multi-solute systems on the adsorption capacity was investigated. Because of thespecific characteristics of ZnS:Mn-NP-AC was found to be efficient for the removal of the dyes studied. The adsorption capacities were investigated and described by the mono- and multi-componentLangmuir and Freundlich isotherm models for both single and binary dye solutions. The isothermconstants for DY12 and RO12 were calculated. For single solution of DY12 and RO12 dyes, the adsorptioncapacities of the applied adsorbent were found to be 90.05 mg/g and 94.52 mg/g, respectively. Equilibrium uptake amounts of DY12 and RO12 in binary solution onto the applied adsorbent werefound to be considerably decreasing with increasing the concentrations of the other dye. A betteragreement between the adsorption equilibrium data and mono-component Langmuir isotherm modelwas found. However, at concentrations within moderate ranges, the extended Freundlich isothermmodel satisfactorily predicted multi-component adsorption equilibrium data. An endothermic and aspontaneous nature for the adsorption of the dyes studied were shown from thermodynamicparameters.