A C1 inhibitor ortholog from rock bream (Oplegnathus fasciatus): Molecular perspectives of a central regulator in terms of its genomic arrangement, transcriptional profiles and anti-protease activities of recombinant peptide

Umasuthan, N.,Bathige, S.D.N.K.,Revathy, K.S.,Wickramaarachchi, W.D.N.,Wan, Q.,Whang, I.,Kim, E.,Park, M.A.,Park, H.C.,Lee, J. Pergamon Press ; Elsevier Science 2014 DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Vol.42 No.2

C1 inhibitor (C1Inh), a member of serpin superfamily, is a crucial regulator of the activation of various plasmatic cascades associated with immunity and inflammation. This study describes the identification and characterization of a C1Inh gene from rock bream Oplegnathus fasciatus (OfC1Inh) at structural, expressional and functional levels. The cDNA-(2245bp) and corresponding gDNA-sequences (5.2kbp) of OfC1Inh were isolated from rock bream transcriptome- and BAC-libraries, respectively. Predicted amino acid sequence of OfC1Inh revealed a two-domain architecture composed of an N-terminal region with two Ig-like domains and a C-terminal region with a serpin domain. Tertiary model of OfC1Inh disclosed its active site topology. In the multi-exonic genomic arrangement of OfC1Inh, it consisted of eleven exons disjoined by ten introns as observed in few other fish homologs. Our comparative analysis indicated that the teleostean C1Inhs were distinct from their non-teleostean vertebrate counterparts in terms of their (1) extended N-terminal domains, (2) evolutionary divergence and (3) exon-intron distribution. The OfC1Inh had a TATA-deficient promoter with a putative initiator element, and two tandemly arranged downstream promoter elements. Several components associated with the immune and inflammatory transcriptional activation were also predicted to exist in 5' flanking region of OfC1Inh. The exclusive mRNA levels in liver and moderate levels in extra-hepatic tissues intimated the diversified importance of OfC1Inh in rock bream physiology. We also provide an evidence for the involvement of OfC1Inh in immune balance, based on its modulated transcription upon different PAMP (lipopolysaccharide and poly I:C)- or pathogen (Streptococcus iniae and rock bream irido virus)-challenges. A recombinantly expressed fusion protein [(r)OfC1Inh] was employed in demonstrating the anti-protease function of OfC1Inh. The (r)OfC1Inh exhibited detectable inhibitory activity against C1 esterase and thrombin, where the anti-C1 esterase role was shown to be potentiated by heparin. Taken together, the results of this study provide the first line of evidence for the possible involvement of a teleostean C1Inh in fish immunity, based on its expressional response(s) and inhibitory properties against two enzymes involved in biological cascades.

Molecular aspects, genomic arrangement and immune responsive mRNA expression profiles of two CXC chemokine receptor homologs (CXCR1 and CXCR2) from rock bream, Oplegnathus fasciatus

Umasuthan, N.,Wan, Q.,Revathy, K.S.,Whang, I.,Noh, J.K.,Kim, S.,Park, M.A.,Lee, J. Academic Press 2014 FISH AND SHELLFISH IMMUNOLOGY Vol.40 No.1

The CXCR1 and CXCR2 are the prototypical receptors and are the only known receptors for mammalian ELR+ (Glu-Leu-Arg) CXC chemokines, including CXCL8 (interleukin 8). These receptors transduce the ELR+ chemokine signals and operate the downstream signaling pathways in inflammation and innate immunity. In this study, we report the identification and characterization of CXCR1 and CXCR2 genes from rock bream fish (OfCXCR1 and OfCXCR2) at the molecular level. The cDNA and genomic DNA sequences of the OfCXCR1 and OfCXCR2 were identified from a transcriptome library and a custom-constructed BAC library, respectively. Both OfCXCR genes consisted of two exons, separated by an intron. The 5'-flanking regions of OfCXCR genes possessed multiple putative transcription factor binding sites related to immune response. The coding sequences of OfCXCR1 and OfCXCR2 encoded putative peptides of 355 and 360 amino acids (aa), respectively. The deduced aa sequences of OfCXCR1 and OfCXCR2 comprised of a G-protein coupled receptors (GPCR) family 1 profile with a GPCR signature and a DRY motif. In addition, seven conserved transmembrane regions were predicted in both OfCXCRs. While our multiple alignment study revealed the functionally significant conserved elements of the OfCXCR1 and OfCXCR2, phylogeny analyses further confirmed their position in teleost sub clade, in which they manifested an evolutionary relatedness with other fish counterparts. Based on comparative analyses, teleost CXC chemokine receptors appear to be distinct from their non-fish orthologs in terms of evolution (both CXCR1 and CXCR2) and genomic organization (CXCR2). Quantitative real-time PCR (qPCR) detected the transcripts of OfCXCR1 and OfCXCR2 in eleven examined tissues, with higher levels in head kidney, kidney and spleen highlighting their crucial importance in immunity. In vitro stimulation of peripheral blood leukocytes (PBLs) with concanavalin A (Con A) resulted in modulation of OfCXCR2 transcription, but not that of OfCXCR1. In addition, the magnitude of the OfCXCR1 and OfCXCR2 transcripts in head kidney and spleen was differentially increased after the in vivo administration of immune stimulants, LPS and poly I:C and in the infection models injected with rock bream irido virus, Edwardsiella tarda and Streptococcus iniae. These lines of evidence suggest that these receptors may play an important role(s) in immune responsive signaling during pathogenesis of rock bream.

Magneto-exciton in a GaN/Ga_1−<i>x</i>Al_<i>x</i>N quantum dot

Revathi, M.,Yoo, ChangKyoo,John Peter, A. Elsevier 2010 Physica E, Low-dimensional systems & nanostructure Vol.43 No.1

<P><B>Abstract</B></P><P>Magneto-exciton bound donor is investigated theoretically in a GaN/Ga<SUB>1−<I>x</I></SUB>Al<SUB><I>x</I></SUB>N quantum dot within the framework of single band effective mass approximation and compared with the experimental results. Binding energies on excitons bound hydrogenic donors and the diamagnetic susceptibility are obtained as a function of dot radius and magnetic field. The valence-band anisotropy is included in our theoretical model using different hole masses in different spatial directions. Zeeman effect is calculated through the energy dependent effective mass. The dependence of donor bound exciton diamagnetic shift is found. The interband optical transition of GaN/Ga<SUB>1−<I>x</I></SUB>Al<SUB><I>x</I></SUB>N dot is computed with various structural parameters in the influence of magnetic field. Our results show that (i) quantum size, the magnetic field and the interband optical transitions have a considerable influence on neutral donor exciton states, (ii) the diamagnetic susceptibility increases with the magnetic field and is not pronounced for smaller dot radii and (iii) the found diamagnetic shift is agreed with the other experimental investigator [Yoichi Yamada et al., 2000 [26]].</P>

Quality of Service using Min-Max Data Size Scheduling in Wireless Sensor Networks

Revathi, A.,Santhi, S.G. International Journal of Computer ScienceNetwork S 2022 International journal of computer science and netw Vol.22 No.9

Wireless Sensor Networks (WSNs) plays an important role in our everyday life. WSN is distributed in all the places. Nowadays WSN devices are developing our world as smart and easy to access and user-friendly. The sensor is connected to all the resources based on the uses of devices and the environment [1]. In WSN, Quality of Service is based on time synchronization and scheduling. Scheduling is important in WSN. The schedule is based on time synchronization. Min-Max data size scheduling is used in this proposed work. It is used to reduce the Delay & Energy. In this proposed work, Two-hop neighboring node is used to reduce energy consumption. Data Scheduling is used to identify the shortest path and transmit the data based on weightage. The data size is identified by three size of measurement Min, Max and Medium. The data transmission is based on time, energy, delivery, etc., the data are sent through the first level shortest path, then the data size medium, the second level shortest path is used to send the data, then the data size is small, it should be sent through the third level shortest path.

A manganese superoxide dismutase with potent antioxidant activity identified from Oplegnathus fasciatus: Genomic structure and transcriptional characterization

Umasuthan, N.,Saranya Revathy, K.,Bathige, S.D.N.K.,Lim, B.S.,Park, M.A.,Whang, I.,Lee, J. Academic Press 2013 Fish & shellfish immunology Vol.34 No.1

In this study, we describe the identification and characterization of manganese superoxide dismutase, an important antioxidant enzyme acting as the chief reactive oxygen species (ROS) scavenger, from rock bream Oplegnathus fasciatus (Of-mMnSOD) at genomic- and transcriptional-levels as well as the biological activity of recombinant protein. The Of-mMnSOD protein portrayed distinct MnSOD family features including signature motifs, metal association sites and the typical active site topology. It was also predicted to be localized in mitochondrial matrix. The Of-mMnSOD had a quinquepartite genome organization encompassing five exons interrupted by four introns. Comparison of its sequence and gene structure with that of other lineages emphasized its strong conservation among different vertebrates. The Of-mMnSOD was ubiquitously transcribed in different rock bream tissues with higher levels in blood cells and metabolically active tissues. Transcription of Of-mMnSOD was kinetically modulated in response to investigational challenges using mitogens (lipopolysaccharide and poly I:C) and live-pathogens (Edwardsiella tarda and rock bream irido virus) in blood cells and liver tissue. The purified recombinant Of-mMnSOD possessed potential antioxidant capacity and actively survived over a range of pH (7.5-11) and temperature (15-40 <SUP>o</SUP>C) conditions. Collectively, findings of this study suggest that Of-mMnSOD combats against oxidative stress and cellular damages induced by mitogen/pathogen-mediated inflammation, by detoxifying harmful ROS (O<SUB>2</SUB><SUP>@?-</SUP>) in rock bream.

A Survey on UAV Network for Secure Communication and Attack Detection: A focus on Q-learning, Blockchain, IRS and mmWave Technologies

Madhuvanthi T,Revathi A 한국인터넷정보학회 2024 KSII Transactions on Internet and Information Syst Vol.18 No.3

Unmanned Aerial Vehicle (UAV) networks, also known as drone networks, have gained significant attention for their potential in various applications, including communication. UAV networks for communication involve using a fleet of drones to establish wireless connectivity and provide communication services in areas where traditional infrastructure is lacking or disrupted. UAV communication networks need to be highly secured to ensure the technology's security and the users' safety. The proposed survey provides a comprehensive overview of the current state-of-the-art UAV network security solutions. In this paper, we analyze the existing literature on UAV security and identify the various types of attacks and the underlying vulnerabilities they exploit. Detailed mitigation techniques and countermeasures for the protection of UAVs are described in this paper. The survey focuses on the implementation of novel technologies like Q-learning, blockchain, IRS, and mmWave. This paper discusses network simulation tools that range in complexity, features, and programming capabilities. Finally, future research directions and challenges are highlighted.

An Optimal Agent Based (Oab) Architecture for Web Service Discovery

Suganya. D,Revathy.A,R.G. Suresh Kumar,N. Moganarangan,D.Madhavan 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.3

Service Discovery is done based on Keyword Match, Simple Semantic Description Services, and Rich Semantic Description Service. Web Services raise the web to a new level by integrating computational aspects. Web Services are accessed by computer programs but these still need help by humans. Web-services should be described in a formal, semantic way, so programs can find, compose and invoke them automatically. Existing system discover the service based on QoS (Quality of Service). It uses the Agent to discover the service, but normalization & classification are not done by Agent here. Existing system are using the selection algorithm, which works in association with QoS value, based on consumer requirements. So In our proposed system we proposed the Normalized, Classification, & Ranking of the QoS parameter based on minimization maximization criteria using agent based architecture. We rank the services according to their QoS levels and their services matching.