Synthesis of Gd2O2S : Eu3+ hollow sphere by a hydrothermal method assisting with reduction route

Jingbao Lian,Fan Liu,Ping Liang,Jianmin Ren,Feng Liu 한양대학교 세라믹연구소 2016 Journal of Ceramic Processing Research Vol.17 No.7

Gd2O2S : Eu3+ hollow spheres were successfully synthesized through a hydrothermal method assisting with reduction routefrom commercially available Gd2O3, Eu2O3, HNO3, (NH4)2SO4 and CO(NH2)2 (urea) as the starting materials. The assynthesizedproducts were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR),scanning electron microscopy (SEM), field emission scanning electron microscopy (FE-SEM) and photoluminescence (PL)spectra. The XRD and FT-IR results demonstrate that the precursor has highly crystalline in nature and could be convertedinto pure Gd2O2S phase at 800 oC for 2 hrs in the hybrid atmosphere of flowing argon and hydrogen (90%Ar + 10%H2). SEMand FE-SEM observations show that the obtained Gd2O2S particles possess hollow sphere structure, which has a typical sizeof about 10 μm in diameter and about 1 μm in shell thickness. PL spectroscopy reveals that the strongest red emission peakis located at 628 nm under 277 nm and 330 nm UV light excitation for the Gd2O2S : Eu3+ (5%mol) hollow spheres, whichcorresponds to the 5D0 → 7F2 transition of Eu3+ ions. Decay study demonstrates that the 5D0 → 7F2 transition of Eu3+ ions hasa single exponential decay behavior. The fitting result shows the lifetime for the 5D0 → 7F2 transition under 277 nm and 330 nmUV light excitation is 0.5692 ms and 0.5435 nm, respectively.

Molecular genetic characterization of rice seed lipoxygenase 3 and assessment of its effects on seed longevity

Qizhang Long,Wenwei Zhang,Peng Wang,Wenbiao Shen,Tong Zhou,Nannan Liu,Ren Wang,Ling Jiang,Jiexue Huang,Yihua Wang,Yuqiang Liu,Jianmin Wan 한국식물학회 2013 Journal of Plant Biology Vol.56 No.4

Lipoxygenases (LOXs) are enzymes involved in lipid peroxidation. Here we reported the identification, molecular and functional characterization of the gene encoding rice (Oryza sativa L.) seed LOX3 (sLOX3). Via a map-based cloning strategy we identified Os03g0700400 as the candidate gene encoding sLOX3. Further functional complementary test and biochemical characterization of the recombinant Os03g0700400 protein verified the identification. The sLOX3 gene was highly expressed in roots, moderately in embryos and very weakly in leaves, leaf sheaths and stems. Transient expression experiment (in rice protoplasts) and subsequent laser confocal microscopic analysis demonstrated that the sLOX3 protein was localized into the cytosol. We next showed that overexpression of sLOX3 in a japonica sLOX3-normal rice cultivar, Wuyunjing 7 accelerated the decrease of seed germination ability when the seeds were routinely stored, which demonstrated that sLOX3 had a negative effect on seed longevity (storability). Meanwhile, an increased occurrence of embryo decay was observed in the same transgenic seeds, suggesting that sLOX3 might negatively affect seed longevity by facilitating colonization of particular seed pathogens. Our result forwarded the understanding of the effects of 9-LOX on rice seed longevity.

A putative plastidial adenine nucleotide transporter, BRITTLE1-3, plays an essential role in regulating chloroplast development in rice (Oryza sativa L.)

Jia Lyu,Yihua Wang,Linglong Liu,Chunming Wang,Yulong Ren,Cheng Peng,Feng Liu,Yunlong Wang,Mei Niu,Di Wang,Ming Zheng,Kunneng Zhou,Shaolu Zhao,Fuqing Wu,Haiyang Wang,Jianmin Wan 한국식물학회 2017 Journal of Plant Biology Vol.60 No.5

Differentiation from proplastids into chloroplasts isa light- and energy-dependent process. How this process isregulated is still poorly understood at the molecular level. We herein report a new putative plastidial adenine nucleotidetransporter, BRITTLE1-3 (referred to as OsBT1-3), encoded bythe rice (Oryza sativa) White Stripe Leaf 2 (WSL2) gene. Loss of OsBT1-3 function results in defective chloroplastbiogenesis, severely reduced photosynthetic efficiency, andfinally a white stripe leaf phenotype in the first four leaves. The expression levels of genes related to chlorophyllbiosynthesis and photosynthesis are drastically reduced,accompanied with over accumulation of reactive oxygenspecies (ROS) in the wsl2 mutant. OsBT1-3 is targeted tothe chloroplasts and it expresses in almost all tissues inplants, especially in young leaves. OsBT1-3 consists of 419amino acids and exhibits features of all mitochondrialcarrier proteins, including a typical transmembrane-spanningdomain and a highly conserved sequence motif designatedas the ‘mitochondrial energy transfer signatures’. Phylogeneticanalysis shows that OsBT1-3 is a putative plastidialadenine nucleotide transporter and is most closely relatedto ZmBT1-2. Together, these observations suggest that thenew putative adenine nucleotide transporter, OsBT1-3,plays an essential role in regulating chloroplast biogenesisand maintenance of ROS homeostasis during rice seedlingde-etiolation.

Removal of malachite green in aqueous solution by adsorption on sawdust

Yinghua Song,Sheguang Ding,Shengming Chen,Hui Xu,Ye Mei,Jianmin Ren 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.12

A new adsorbent was synthesized from sawdust, a forest residue, in which methanol was used as a solvent and triethylamine as a modification agent under the following optimum conditions: 25 oC of reaction temperature, 1 : 8.75 of the ratio of sawdust to triethylamine (g :mL) and 1 hour of reaction time. The adsorption capacity of this adsorbent for malachite green was improved by 632.98% in contrast to that of the unmodified sawdust under the same adsorption conditions. Factors affecting the adsorption behavior of this adsorbent for malachite green, such as pH value, adsorption time, temperature and initial dye concentration, were evaluated through experiments in a batch system. The results indicated that the maximum adsorption capacity can be achieved at 5.08 of pH value and adsorption equilibrium can be reached in 6 hours. It was also found that the higher the temperature, the higher the adsorptive capacity would be. The Freundlich isotherm model provides a better description for the adsorption equilibrium when compared with the Langmuir equation in the conditions of the present study. Additionally, to examine the controlling mechanisms of the process, kinetic equations of the mass transfer and chemical reaction, the pseudo-first order model, the pseudo-second order model and the intraparticle diffusion model were used to correlate the experimental data respectively. The adsorption process of malachite green on sawdust tended to be controlled simultaneously by film mass transfer and intra-particle diffusion and it accompanied chemical reactions. It showed that the sawdust modified with triethylamine had good performance for cationic dye and can be used as a biomass adsorbent to treat dyes-containing wastewater with high quality.

Catalytic combustion of volatile aromatic compounds over CuO-CeO2 catalyst

Hongmei Xie,Qinxiang Du,Hui Li,Guilin Zhou,Shengming Chen,Zhaojie Jiao,Jianmin Ren 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.7

Ce1−xCuxO2 oxide solid solution catalysts with different Ce/Cu mole ratios were synthesized by the one-pot complex method. The prepared Ce1−xCuxO2 catalysts were characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and H2 temperature-programmed reduction (H2-TPR). Their catalytic properties were also investigated by catalytic combustion of phenyl volatile organic compounds (PVOCs: benzene, toluene, xylene, and ethylbenzene) in air. XRD analysis confirmed that the CuO species can fully dissolve into the CeO2 lattice to form CeCu oxide solid solutions. XPS and H2-TPR results indicated that the prepared Ce1−xCuxO2 catalysts contain abundant reactive oxygen species and superior reducibility. Furthermore, the physicochemical properties of the prepared Ce1−xCuxO2 catalysts are affected by the Ce/Cu mole ratio. The CeCu3 catalyst with Ce/Cu mole ratio of 3.0 contains abundant reactive oxygen species and exhibits superior catalytic combustion activity of PVOCs. Moreover, the ignitability of PVOCs is also affected by the respective physicochemical properties. The catalytic combustion conversions of ethylbenzene, xylene, toluene, and benzene are 99%, 98.9%, 94.3%, and 62.8% at 205, 220, 225, and 225 oC, respectively.

Identification a novel de novo RUNX2 frameshift mutation associated with cleidocranial dysplasia

Gong Lei,Odilov Bekzod,Han Feng,Liu Fuqiang,Sun Yujing,Zhang Ningxin,Zuo Xiaolin,Yang Jiaojiao,Wang Shouyu,Hou Xinguo,Ren Jianmin 한국유전학회 2022 Genes & Genomics Vol.44 No.6

Background: Cleidocranial dysplasia (CCD) is a rare genetic disorder affecting bone and cartilage development. Clinical features of CCD comprise short stature, delayed ossification of craniofacial structures with numerous Wormian bones, underdeveloped or aplastic clavicles and multiple dental anomalies. Several studies have revealed that CCD development is strongly linked with different mutations in runt-related transcription factor 2 (RUNX2) gene. Objective: Identification and functional characterization of RUNX2 mutation associated with CCD. Methods: We performed genetic testing of a patient with CCD using whole exome sequencing and found a novel RUNX2 frameshift mutation: c.1550delT in a sporadic case. We also compared the functional activity of the mutant and wild-type RUNX2 through immunofluorescence microscopy and osteocalcin promoter luciferase assay. Results: We found a novel RUNX2 frameshift mutation, c.1550delT (p.Trp518Glyfs*60). Both mutant RUNX2 and wild-type RUNX2 protein were similarly confined in the nuclei. The novel mutation caused abrogative transactivation activity of RUNX2 on osteocalcin promoter. Conclusions: We explored a novel RUNX2 deletion/frameshift mutation in a sporadic CCD patient. This finding suggests that the VWRPY domain may play a key role in RUNX2 transactivation ability.