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RISS 인기검색어
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- 저자 : Reamtong, Onrapak (검색결과 3 건)
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Peerut Chienwichai,Supachai Topanurak,Onrapak Reamtong,Usa Boonyuen,Suwalee Worakhunpiset,Prapin Tharnpoophasiam 대한독성 유전단백체 학회 2018 Molecular & cellular toxicology Vol.14 No.1
Bisphenol A (BPA) is an environmental toxicant that causes adverse effects to liver even at low concentrations. Non-alcoholic fatty liver disease (NAFLD) presents as significant accumulation of lipids in the liver, impairing detoxification processes. Nevertheless, little information is available regarding the susceptibility of NAFLD to BPA toxicity. Here, we compared the effects of low concentration BPA exposure on the protein profiles of normal and NAFLD cells. We established NAFLD model by treating HepG2 cells with fatty acids and subsequently treated with low BPA concentration. Protein expression profiles showed that four proteins from normal cells and nine proteins from NAFLD cells were dysregulated after BPA treatment. A pathway analysis revealed decreased expression of translation elongation factor proteins in NAFLD cells following BPA treatment and this down-regulation was confirmed by immunoblotting. Our findings suggest that BPA toxicity is more deleterious, especially to translation elongation, in NAFLD cells than normal cells
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Nitat Sookrung,Thanyathon Khetsuphan,Urai Chaisri,Nitaya Indrawattana,Onrapak Reamtong,Wanpen Chaicumpa,Anchalee Tungtrongchitr 대한천식알레르기학회 2014 Allergy, Asthma & Immunology Research Vol.6 No.4
Purpose: Cockroach (CR) is a common source of indoor allergens, and Per a 1 is a major American CR (Periplaneta americana) allergen; however,several attributes of this protein remain unknown. This study identifies a novel specific B cell epitope and anatomical locations of Per a 1.0105. Methods:Recombinant Per a 1.0105 (rPer a 1.0105) was used as BALB/c mouse immunogen for the production of monoclonal antibodies (MAb). TheMAb specific B cell epitope was identified by determining phage mimotopic peptides and pair-wise alignment of the peptides with the rPer a 1.0105amino acid sequence. Locations of the Per a 1.0105 in P. americana were investigated by immunohistochemical staining. Results: The rPer a1.0105 (~13 kDa) had 100%, 98% and ≥90% identity to Per a 1.0105, Per a 1.0101, and Cr-PII, respectively. The B-cell epitope of the Per a 1.0105specific-MAb was located at residues QDLLLQLRDKGV110 contained in all 5 Per a 1.01 isoforms and Per a 1.02. The epitope was analogous to theBla g 1.02 epitope; however, this B-cell epitope was not an IgE inducer. Per a 1.0105 was found in the midgut and intestinal content of American CRbut not in the other organs. The amount of the Per a 1 was ~544 μg per gram of feces. Conclusions: The novel Per a 1 B-cell epitope described inthis study is a useful target for allergen quantification in samples; however, the specific MAb can be used as an allergen detection reagent. TheMAb based-affinity resin can be made for allergen purification, and the so-purified protein can serve as a standard and diagnostic allergen as wellas a therapeutic vaccine component. The finding that the Per a 1 is contained in the midgut and feces is useful to increase yield and purity whenpreparing this allergen.
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ALCAM is a Novel Cytoplasmic Membrane Protein in TNF-α Stimulated Invasive Cholangiocarcinoma Cells
Adisakwattana, Poom,Suwandittakul, Nantana,Petmitr, Songsak,Wongkham, Sopit,Sangvanich, Polkit,Reamtong, Onrapak Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9
Background: Cholangiocarcinoma (CCA), or bile duct cancer, is incurable with a high mortality rate due to a lack of effective early diagnosis and treatment. Identifying cytoplasmic membrane proteins of invasive CCA that facilitate cancer progression would contribute toward the development of novel tumor markers and effective chemotherapy. Materials and Methods: An invasive CCA cell line (KKU-100) was stimulated using TNF-${\alpha}$ and then biotinylated and purified for mass spectrometry analysis. Novel proteins expressed were selected and their mRNAs expression levels were determined by real-time RT-PCR. In addition, the expression of ALCAM was selected for further observation by Western blot analysis, immunofluorescent imaging, and antibody neutralization assay. Results: After comparing the proteomics profile of TNF-${\alpha}$ induced invasive with non-treated control cells, over-expression of seven novel proteins was observed in the cytoplasmic membrane of TNF-${\alpha}$ stimulated CCA cells. Among these, ALCAM is a novel candidate which showed significant higher mRNA- and protein levels. Immunofluorescent assay also supported that ALCAM was expressed on the cell membrane of the cancer, with increasing intensity associated with TNF-${\alpha}$. Conclusions: This study indicated that ALCAM may be a novel protein candidate expressed on cytoplasmic membranes of invasive CCA cells that could be used as a biomarker for development of diagnosis, prognosis, and drug or antibody-based targeted therapies in the future.
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