Cell-Selective Metabolic Glycan Labeling Based on Ligand-Targeted Liposomes

Ran Xie,Senlian Hong,Lianshun Feng,Jie Rong,Xing Chen 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1

Cell-surface glycans play key roles in mediating various molecular recognition events; aberrant glycosylation is implicated in disease progression. Therefore, probing the dynamic changes of glycan biosynthesis and structures is of great importance for augmenting our understanding of glycobiology and improving disease diagnosis and therapeutics. The metabolic glycan labeling was an appealing approach to incorporate specially designed carbohydrate analogs into the glycans, which enables the detection and imaging of the glycans in cells and living animals. However, one major bottleneck of this method is cell- type selectivity. Herein, we present the cell-specific metabolic glycan labeling using ligand-targeted liposomes to deliver unnatural sugars to target cells in a cell-surface receptor dependent manner. In this strategy, sugar analogs are encapsulated in ligand-targeted liposomes. The ligands bind to specific cell-surface receptors that are only expressed or up-regulated in target cells, which mediate the intracellular delivery of azidosugars via endocytosis. The delivered azidosugars are metabolically incorporated into cell-surface glycans and detected or imaged using a bioorthogonal reaction. The application of this strategy includes the facile introduction of myriad alternative ligands, as well as the cell-specific or tissue-specific imaging and detection of glycosylation in vivo.

Visual Fatigue Reduction Based on Depth Adjustment for DIBR System

( Ran Liu ),( Yingchun Tan ),( Fengchun Tian ),( Hui Xie ),( Guoqin Tai ),( Weimin Tan ),( Junling Liu ),( Xiaoyan Xu ),( Chaibou Kadri ),( Naana Abakah ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.4

A depth adjustment method for visual fatigue reduction for depth-image-based rendering (DIBR) system is proposed. One important aspect of the method is that no calibration parameters are needed for adjustment. By analyzing 3D image warping, the perceived depth is expressed as a function of three adjustable parameters: virtual view number, scale factor and depth value of zero parallax setting (ZPS) plane. Adjusting these three parameters according to the proposed parameter modification algorithm when performing 3D image warping can effectively change the perceived depth of stereo pairs generated in DIBR system. As the depth adjustment is performed in simple 3D image warping equations, the proposed method is facilitative for hardware implementation. Experimental results show that the proposed depth adjustment method provides an improvement in visual comfort of stereo pairs as well as generating comfortable stereoscopic images with different perceived depths that people desire.

An Efficient Dynamic Group Signature with Non-frameability

( Run Xie ),( Chunxiang Xu ),( Chanlian He ),( Xiaojun Zhang ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.5

A group signature scheme allows any member to sign on behalf of a group. It is applied to practical distributed security communication environments, such as privacy-preserving, data mining. In particular, the excellent features of group signatures, including membership joining and revocation, anonymity, traceability, non-frameability and controllable linkability, make group signature scheme more attractive. Among these features, non-frameability can guarantee that a member`s signature cannot be forged by any other (including issuer), and controllable linkability supports to confirm whether or not two group signatures are created by the same signer while preserving anonymity. Until now, only Hwang et al.`s group schemes (proposed in 2013 and 2015) can support all of these features. In this paper, we present a new dynamic group signature scheme which can achieve all of the above excellent features. Compared with their schemes, our scheme has the following advantages. Firstly, our scheme achieves more efficient membership revocation, signing and verifying. The cost of update key in our scheme is two-thirds of them. Secondly, the tracing algorithm is simpler, since the signer can be determined without the judging step. Furthermore, in our scheme, the size of group public key and member`s private key are shorter. Lastly, we also prove security features of our scheme, such as anonymity, traceability, non-frameability, under a random oracle model.

Hole-filling Based on Disparity Map for DIBR

( Ran Liu ),( Hui Xie ),( Fengchun Tian ),( Yingjian Wu ),( Guoqin Tai ),( Yingchun Tan ),( Weimin Tan ),( Bole Li ),( Hengxin Chen ),( Liang Ge ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.10

Due to sharp depth transition, big holes may be found in the novel view that is synthesized by depth-image-based rendering (DIBR). A hole-filling method based on disparity map is proposed. One important aspect of the method is that the disparity map of destination image is used for hole-filling, instead of the depth image of reference image. Firstly, the big hole detection based on disparity map is conducted, and the start point and the end point of the hole are recorded. Then foreground pixels and background pixels are distinguished for hole-dilating according to disparity map, so that areas with matching errors can be determined and eliminated. In addition, parallaxes of pixels in the area with holes and matching errors are changed to new values. Finally, holes are filled with background pixels from reference image according to these new parallaxes. Experimental results show that the quality of the new view after hole-filling is quite well; and geometric distortions are avoided in destination image, in contrast to the virtual view generated by depth-smoothing methods and image inpainting methods. Moreover, this method is easy for hardware implementation.

Selection of Reference Genes for Real-time Quantitative PCR Normalization in the Process of Gaeumannomyces graminis var. tritici Infecting Wheat

Xie, Li-hua,Quan, Xin,Zhang, Jie,Yang, Yan-yan,Sun, Run-hong,Xia, Ming-cong,Xue, Bao-guo,Wu, Chao,Han, Xiao-yun,Xue, Ya-nan,Yang, Li-rong The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.1

Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression stability of these nine candidates was determined with three programs-geNorm, Norm Finder, and Best Keeper. $TUB{\beta}$ was identified as the most stable reference gene. Furthermore, the exopolygalacturonase gene (ExoPG) was selected to verify the reliability of $TUB{\beta}$ expression. The expression profile of ExoPG assessed using $TUB{\beta}$ agreed with the results of digital gene expression analysis by RNA-Seq. This study is the first systematic exploration of the optimal reference genes in the infection process of Gaeumannomyces graminis var. tritici.

Micro-grinding of Micro-groove Array on Tool Rake Surface for Dry Cutting of Titanium Alloy

Jin Xie,Min-Jian Luo,Jian-Lin He,Xu-Ran Liu,Ting-Wu Tan 한국정밀공학회 2012 International Journal of Precision Engineering and Vol. No.

A micro-grinding approach is proposed to fabricate micro-groove array on tool rake surface. These micro-grooves were used to rapidly dissipate cutting chips and heat from cutting zone for eco-cutting of titanium alloy without any coolant. This tool is called micro-grooved tool. The objective is to replace chemical and high-energy-beam etching approaches because they have not yet been able to control micron-scale groove shape accuracy. In this study, a superhard diamond wheel V-tip was employed to perform a micro-grinding through a traverse grinding. After micro-grinding, these micro-grooves were characterized by the mean depth of 155.1 μm, the mean form error PV of 7.8 μm and the mean tip radius of 45.6 μm. Firstly, traditional plate tool, orthogonal micro-grooved tool and diagonal micro-grooved tool were employed to perform a dry turning of titanium alloy, respectively; then, tool wear, surface roughness, chip topography and shear angle were investigated. It is shown that the sharpened diamond wheel V-tip is valid to grinding micro-groove array on tool rake surface without any burrs. Furthermore, the micro-grooved tools may reduce the cutting sparks, tool wear, 3D chip size and chip saw-tooth size compared to traditional plate tool. The diagonal micro-grooved tool may decrease tool wear by 6.7% and surface roughness by 37.3% and increase shear angle by 24.3% against orthogonal micro-grooved tool, respectively. This is because the diagonal micro-grooving direction is identical to the cutting chip flowing on tool rake surface. It is confirmed that the diagonal micro-grooved tool is applicable to a stable dry cutting of titanium alloy.

Biocatalysis and Fermentation Technology : Thermostable Sites and Catalytic Characterization of Xylanase XYNB of Aspergillus niger SCTCC 400264

( Xin Ran Li ),( Hui Xu ),( Jie Xie ),( Qiao Fu Yi ),( Wei Li ),( Dai Rong Qiao ),( Yi Cao ),( Yu Cao ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.4

In order to improve the expression of heat-resistant xylanase XYNB from Aspergillus niger SCTCC 400264, XynB has been cloned into Pichia pastoris secretary vector pPIC9K. The XynB production of recombinant P. pastoris was four times that of E. coli, and the Vmax and specific activity of XynB reached 2,547.7 umol/mg and 4,757 U/mg, respectively. XynB still had 74% residual enzyme activity after 30 min of heat treatment at 80°C. From the van der Waals force analysis of XYNB (ACN89393 and AAS67299), there is one more oxygen radical in AAS67299 in their catalytic site, indicating that the local cavity is much more free, and it is more optimal for substrate binding, affinity reaction, and proton transfer, etc, and e ventually i ncreasing enzyme activity. The H-bonds analysis of XYNB indicated that there are two more H-bonds in the 33rd Ser of XYNB (AAS67299) than in the 33rd Ala(ACN89393 ), and two H-bonds between Ser70 and Asp67.

The Study on Contents of Vitamin D in Frequently Consumed Food

Yong-Xie Jin,Su Hui Park,Mi Ran Yang,Hye Kyung Han,Ae-Jin Choi,Sang Hoon Lee,Youngmin Choi 한국식품영양과학회 2017 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

Positioning Reference Signal-Orientated Moving Train Localization in Ricean Channel

Cui-Ran Li,Jian-Li Xie 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.10

In this paper, the challenging problem of train localization is investigated. Precise localization of rail vehicles is a key element for development of more efficient and safe operation of high-speed railway. The localization task is formulated in a 3GPP LTE-based framework that enables us to derive the Doppler frequency offset of moving train, by exploitation of positioning reference signal (PRS) placed in the subframe, in Ricean channel. Then, a PRS-oriented train position approach is proposed. The PRS time difference of arrival from positioning references eNB1 and eNB2, is calculated by sequences cross correlation, and the train running curve is determined by inquiring about the line database of track section. Finally, the train position is evaluated by combining the information of PRS time difference of arrival and the train’s moving trajectory. In the simulation, the localization performance is well demonstrated, when two types of railway alignments, i.e., straight line and circle curve, are considered.

Study on 3D Characterized Profile and Point Accuracies of Ground Micro-Pyramid-Structured Si Surface

Jin Xie,Yong-Xian Lu,Xu-Ran Liu,Yan-Jun Lu 한국정밀공학회 2013 International Journal of Precision Engineering and Vol. No.

It is very difficult to evaluate 3D profile accuracy of micro-machined surface due to the 3D characterization of its measured point cloud. Hence, three ideal point cloud models, which are grid point model, slash profile model and horizontal profile model, were constructed to register 3D measured point cloud of micro-machined surface and evaluate the accuracies of characterized profile and points. First, #400 and #600 diamond wheel V-tips were employed to fabricate non-integrated and integrated micro-pyramidstructured surfaces with 173 μm in depth and 0.865 in aspect ratio in Computer Numerical Control (CNC) grinding system,respectively; then a white light interferometer was utilized to measure the micro-ground surfaces; finally, registration accuracy and registration efficiency were investigated with regard to characterized profile and point accuracies. The results show that registration accuracy increases and registration efficiency decreases with increasing model point number, but they have little relation with the posture of measured point cloud. Registration error may converge to micro-ground form error when model point number is larger than measured point number. Moreover, the slash profile and horizontal profile models may register characterized profile and point more precisely than grid point model. The slash profile model can produce the best registration accuracy and efficiency. It is confirmed that the micro-ground form errors are 23.8 μm and 7.9 μm, the characterized profile errors are 37.2 μm and 19.0 μm and the characterized peak errors are 51.2 μm and 34.1 μm for non-integrated surface and integrated surface, respectively. As a result,a #600 diamond grinding wheel may be used to perform a precision micro-grinding in CNC grinding system.