Neuro-Fuzzy Modelling and Stable PD Controller for Angular Position in Steering Systems

Ramos-Fernández Julio C.,López-Morales Virgilio,Márquez-Vera Marco A.,Pérez Juan Manual Xicotencatl,Suarez-Cansino Joel 한국자동차공학회 2021 International journal of automotive technology Vol.22 No.6

The precision agriculture and soil tillage are tasks which can be achieved by Automated Tractors (AT) through the integration of several servomechanisms. In order to reach a high autonomy under various work conditions of the AT, control laws’ design and tuning are paramount. The aim of this work is to develop an angle position controller for steering systems applied to an AT. We introduce an automatic electronic steering system by using a fuzzy model, obtained through an Adaptive Neuro-Fuzzy Inference System (ANFIS) algorithm training. A proportional derivative (PD) controller is also tuned through a Takagi-Sugeno fuzzy model (T-S). Furthermore, several closed-loop stability tests were carried out, in numerical simulation and real-time implementation. The feasibility of our methodology is illustrated through the tracking of several angles’ profiles in a real test scenario. For the tracking of a trajectory or several set-points, we have obtained mean errors about 0.6545 and 0.8651 degrees, respectively. Some mechatronic integrations to convert a conventional tractor into a basic Autonomous Agriculture Off-road Tractor (AAOT) are also shown.

Effects of Processing Conditions on the Protein Quality of Fried Anchovy Kamaboko Engraulis japonica

Ramos, Leny R. Ordonez,Choi, Nam-Do,Ryu, Hong-Soo The Korean Society of Fisheries and Aquatic Scienc 2012 Fisheries and Aquatic Sciences Vol.15 No.4

The effects of processing and frozen storage conditions on the quality of anchovy Engraulis japonica fried surimi gels were investigated. Protein content decreased after surimi gel processing from 19.6% (raw meat) to 12.1% (kamaboko) due to the added ingredients and change in water content. Lipid content decreased from 2.8% (raw meat) to 1.3% in minced and 0.5% in surimi, but fried kamaboko showed a 6.9 % lipid level. Thiobarbituric acid values and thiobarbituric acid reactive substances levels were highest in kamaboko samples, 89.5 and 1.9 mg/g solid, and increased gradually with storage time to 101.8 and 4.6 mg/g solid, respectively. In vitro protein digestibility increased from 79.2% in raw anchovy to 88.5% in kamaboko samples. Levels of trypsin inhibitor decreased gradually with processing and during storage time from 2.43 in raw anchovy to 0.31 mg/g solid in the kamaboko sample after 60 days of frozen storage. No noticeable changes in total essential amino acid was observed during processing conditions. Computed protein efficiency ratio for kamaboko was highest (2.59) compared with whole anchovy (1.96), minced (1.94) and surimi (2.50). Fresh fried anchovy kamaboko showed similar values of hardness, springiness, gumminess and chewiness to commercial surimi gel, but a higher values were seen for fracturability and adhesiveness, and lower values for cohesiveness and resilience. The frozen and thawed anchovy kamaboko showed higher values for all of these rheological parameters compared with fresh and commercial kamaboko. Anchovy kamaboko showed the lowest lightness (62.9) and redness (0.16) and similar yellowness (11.9) compared with commercial kamaboko. Frozen storage and vacuum packaging were effective maintaining the shelf life of anchovy kamaboko within 30 days, but were not effective after 45 days due to fat oxidation.

Integration of Genome-wide Approaches Identifies lncRNAs of Adult Neural Stem Cells and Their Progeny In Vivo

Ramos, Alexander D.,Diaz, A.,Nellore, A.,Delgado, Ryan N.,Park, K.Y.,Gonzales-Roybal, G.,Oldham, Michael C.,Song, Jun S.,Lim, Daniel A. Cell Press 2013 Cell stem cell Vol.12 No.5

Long noncoding RNAs (lncRNAs) have been described in cell lines and various whole tissues, but lncRNA analysis of development in vivo is limited. Here, we comprehensively analyze lncRNA expression for the adult mouse subventricular zone neural stem cell lineage. We utilize complementary genome-wide techniques including RNA-seq, RNA CaptureSeq, and ChIP-seq to associate specific lncRNAs with neural cell types, developmental processes, and human disease states. By integrating data from chromatin state maps, custom microarrays, and FACS purification of the subventricular zone lineage, we stringently identify lncRNAs with potential roles in adult neurogenesis. shRNA-mediated knockdown of two such lncRNAs, Six3os and Dlx1as, indicate roles for lncRNAs in the glial-neuronal lineage specification of multipotent adult stem cells. Our data and workflow thus provide a uniquely coherent in vivo lncRNA analysis and form the foundation of a user-friendly online resource for the study of lncRNAs in development and disease.

Modeling flow instability of an Algerian sand with the dilatancy rule in CASM

Ramos, Catarina,Fonseca, Antonio Viana da,Vaunat, Jean Techno-Press 2015 Geomechanics & engineering Vol.9 No.6

The aim of the present work was the study of instability in a loose sand from Les Dunes beach in Ain Beninan, Algeria, where the Boumerdes earthquake occurred in 2003. This earthquake caused significant structural damages and claimed the lives of many people. Damages caused to infrastructures were strongly related to phenomena of liquefaction. The study was based on the results of two drained and six undrained triaxial tests over a local sand collected in a region where liquefaction occurred. All the tests hereby analyzed followed compression stress-paths in monotonic conditions and the specimens were isotropically consolidated, since the objective was to study the instability due to static loading as part of a more general project, which also included cyclic studies. The instability was modeled with the second-order work increment criterion. The definition of the instability line for Les Dunes sand and its relation with yield surfaces allowed the identification of the region of potential instability and helped in the evaluation of the susceptibility of soils to liquefy under undrained conditions and its modeling. The dilatancy rate was studied in the points where instability began. Some mixed tests were also simulated, starting with drained conditions and then changing to undrained conditions at different time steps.

The effect of mechanical properties of bone in the mandible, a numerical case study

Ramos, Antonio,Marques, Hugo,Mesnard, Michel Techno-Press 2014 Advances in biomechanics & applications Vol.1 No.1

Bone properties are one of the key components when constructing models that can simulate the mechanical behavior of a mandible. Due to the complexity of the structure, the tooth, ligaments, different bones etc., some simplifications are often considered and bone properties are one of them. The objective of this study is to understand if a simplification of the problem is possible and assess its influence on mandible behavior. A cadaveric toothless mandible was used to build three computational models from CT scan information: a full cortical bone model; a cortical and cancellous bone model, and a model where the Young's modulus was obtained as function of the pixel value in a CT scan. Twelve muscle forces were applied on the mandible. Results showed that although all the models presented the same type of global behavior and proximity in some locations, the influence of cancellous bone can be seen in strain distribution. The different Young's modulus defined by the CT scan gray scale influenced the maximum and minimum strains. For modeling general behavior, a full cortical bone model can be effective. However, when cancellous bone is included, maximum values in thin regions increase the strain distribution. Results revealed that when properties are assigned to the gray scale some peaks could occur which did not represent the real situation.

Overexpression and secretion of AgaA7 from Pseudoalteromonas hodoensis sp. nov in Bacillus subtilis for the depolymerization of agarose

Ramos, K.R.M.,Valdehuesa, K.N.G.,Cabulong, R.B.,Moron, L.S.,Nisola, G.M.,Hong, S.K.,Lee, W.K.,Chung, W.J. IPC Science and Technology Press ; Elsevier Scienc 2016 Enzyme and microbial technology Vol.90 No.-

<P>Interest in agar or agarose-based pharmaceutical products has driven the search for potent agarolytic enzymes. An extracellular beta-agarase (AgaA7) recently isolated from Pseudoalteromonas hodoensis sp. nov was expressed in Bacillus subtilis, which was chosen due to its capability to overproduce and secrete functional enzymes. Phenotypic analysis showed that the engineered B. subtilis secreted a functional AgaA7 when fused with the aprE signal peptide (SP) at the amino-terminus. The maximum agarolytic activity was observed during the late logarithmic phase. To further improve the secretion of AgaA7, an expression library of AgaA7 fused to different naturally occurring B. subtilis SPs was created. The amount of AgaA7 secreted by the clones was compared through activity assay, immuno-blot, and purification via affinity chromatography. Although the aprE SP can readily facilitate the secretion of AgaA7, other SPs such as yggA, pel, and lipA were relatively more efficient. Among these SPs, lipA was the most efficient in improving the secretion of AgaA7.The use of B. subtilis as host for the expression and secretion of agarolytic and other hydrolytic enzymes can be a useful tool in the field of white biotechnology. (C) 2016 Elsevier Inc. All rights reserved.</P>

Combining De Ley–Doudoroff and methylerythritol phosphate pathways for enhanced isoprene biosynthesis from d-galactose

Ramos, Kristine Rose M.,Valdehuesa, Kris Niñ,o G.,Liu, Huaiwei,Nisola, Grace M.,Lee, Won-Keun,Chung, Wook-Jin Springer-Verlag 2014 BIOPROCESS AND BIOSYSTEMS ENGINEERING Vol.37 No.12

Effects on the Use of Two Textbooks for Four Types of Classes in a South Korean University

Ramos, Ian Done D. The International Promotion Agency of Culture Tech 2013 International Journal of Advanced Culture Technolo Vol.1 No.2

This paper determined students' ranks of difficulty on the use of materials in terms of 1) understanding the layout of the learning materials, 2) reading comprehension of the learning materials, and 3) realization on relevance to needs of the learning materials. It also determined students' 4) rank and frequency of attitude on the materials. With the data gathered through 128 survey questionnaires, 7 focused group discussions, and 10 interviews, the results were found out that there was an inappropriate assessment procedure set by this particular university. The researcher concludes that: 1) design of four types of classes by just using the two textbooks with their respective workbooks is grammar-based with limited conversation activities; 2) placement for these students in one big class size was implemented without considering their common interest and motivation and language levels; and, 3) qualification of teachers teaching these EFL students did not support students' real needs and the language program itself. Content professors who were made to teach may have the ability to input learning, but their teaching styles may differ from the ones who are real English teachers. This paper then recommends that teachers and school administration should have an appropriate placement exam before students attend the class, especially in a big class size. There could only be a few problems among students in one big class size when students' level of competence is proportioned. With this, topics and conversation activities can even be more flexible with the maneuver of art of questioning, various dimensions of thinking, strategic competence, learning attitude or behavior, etc. to ensure sustenance of communicative mode and level of interest and motivation in the classroom. Grammar-based instruction can only be taught when a need arises. Thus, the course description of each class will be able to transact the objectives ready for developing students' communication competence. Moreover, proper measurement can be utilized to validly assess the amount of students' learning and the progress of language curriculum design in terms of materials selection and teaching approach.

Nanostructured porous graphene and its composites for energy storage applications

Ramos Ferrer Pablo,Mace Annsley,Thomas Samantha N.,Jeon Ju-Won 나노기술연구협의회 2017 Nano Convergence Vol.4 No.29

Graphene, 2D atomic-layer of sp2 carbon, has attracted a great deal of interest for use in solar cells, LEDs, electronic skin, touchscreens, energy storage devices, and microelectronics. This is due to excellent properties of graphene, such as a high theoretical surface area, electrical conductivity, and mechanical strength. The fundamental structure of graphene is also manipulatable, allowing for the formation of an even more extraordinary material, porous graphene. Porous graphene structures can be categorized as microporous, mesoporous, or macroporous depending on the pore size, all with their own unique advantages. These characteristics of graphene, which are further explained in this paper, may be the key to greatly improving a wide range of applications in energy storage systems.

Use of inductors in the control of Colletotrichum gloeosporioides and Rhizopus stolonifer isolated from soursop fruits: in vitro tests

Ramos-Guerrero, Anelsy,Gonzalez-Estrada, Ramses Ramon,Hanako-Rosas, Greta,Bautista-Banos, Silvia,Acevedo-Hernandez, Gustavo,Tiznado-Hernandez, Martin Ernesto,Gutierrez-Martinez, Porfirio 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.3

Soursop (Annona muricata) is a tropical fruit that can be infected by Colletotrichum gloeosporioides and Rhizopus stolonifer. Traditional methods used for postharvest disease control include the application of fungicides, however due to their excessive use, as well as their persistence in the environment, the development of new strategies that control pathogens are required. The application of chitosan (Chi), salicylic acid (SA) and methyl jasmonate (MJ) is an environmentally-friendly alternative with antimicrobial properties and also induces defense mechanisms in plant tissues. In this study, Colletotrichum was reactivated and Rhizopus was identified using morphological features and molecular tools. In vitro, the application of 0.5 and 1.0% of Chi alone or in combination with SA and MJ decreased mycelial growth and sporulation, a complete inhibition of spore germination was obtained. Thus, the application of Chi in combination with SA and MJ could be a smart strategy to inhibit the development of pathogens that attack soursop fruit.