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        Analysis of the Bacterial Community in a Full-scale Printing and Dyeing Wastewater Treatment System Based on T-RFLP and 454 Pyrosequencing

        Qingxiang Yang,Jia Wang,Xinkuan Han,Yuanyuan Xu,Dong Liu,Hongxin Hao,Xuemei L,Yuhui Guo,Tianqi Niu,Shiyue Qi 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.1

        In this study, the bacterial dynamics andstructure compositions in the two-stage biological processof a full-scale printing and dyeing wastewater (PDW)treatment system were traced and analyzed by terminalrestriction fragment length polymorphism (T-RFLP) and454 pyrosequencing techniques. T-RFLP analysis showedthat the microbial communities experienced significantvariation in the process of seed sludge adaptation to thePDW environments and were in constant evolution duringthe whole running period of the system, despite theconstant COD and color removal effects. Pyrosequencingresults indicated that the two-stage biological systemharbored rather diverse bacteria, with Proteobacteria beingthe predominant phylum during the steady running period,although its microbial compositions differed. The firststageaerobic tank was dominated by α-Proteobacteria(89.05% of Proteobacteria), whereas in the second-stageaerobic tank, β- and γ-Proteobacteria, besides α-Proteobacteria,were the dominant bacterial populations.

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        Crystallographic Characterizations of Eutectic and Secondary Carbides in a Fe-12Cr-2.5Mo-1.5W-3V-1.25C Alloy

        Jing Guo,Ligang Liu,Yunli Feng,Sha Liu,Xuejun Ren,Qingxiang Yang 대한금속·재료학회 2017 METALS AND MATERIALS International Vol.23 No.2

        In this work, the morphology and structures of the eutectic and secondary carbides in a new high chromiumFe-12Cr-2.5Mo-1.5W-3V-1.25C designed for cold-rolling work roll were systematically studied. The precipitatedcarbides inside the grains and along the grain boundaries were investigated with optical microscope,scanning electron microscopy with energy dispersive spectroscopy, transmission electron microscopy andX-Ray diffraction. Selected area diffraction patterns have been successfully used to identify the crystal formationand lattice constants of the carbides with different alloying elements. The results show that the eutectic carbidesprecipitated contain MC and M2C distributed along the grain boundaries with dendrite feature. The compositionand crystal structure analysis shows that the eutectic MC carbides contain VC and WC with a cubic and hexagonalcrystal lattice structures respectively, while the eutectic M2C carbides predominantly contain V2C and Mo2Cwith orthorhombic and hexagonal crystal lattices respectively. The secondary carbides contain MC, M2C,M7C3 formed along the grain boundaries and their sizes are much larger than the eutectic carbides ones. The secondary M23C6 is much small (0.3-0.5μm) and is distributed dispersively inside the grain. Similar tothe eutectic carbides, the secondary carbides also contain VC, WC, V2C, and Mo2C. M7C3 is hexagonal(Fe,Cr)7C3, while M23C6 is indexed to be in a cubic crystal form.

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